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اثر متیل جاسمونات برمیزان تولید ترکیبات زیست‌فعال در ریشه موئین شیرین بیان (Glycyrrhiza glabra) | ||
| علوم باغبانی ایران | ||
| دوره 56، شماره 1، فروردین 1404، صفحه 1-17 اصل مقاله (1.79 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijhs.2024.380834.2200 | ||
| نویسندگان | ||
| مریم اله دو* 1؛ لیلا مهرآوران2؛ حمیده خواجه3 | ||
| 1گروه اصلاح نباتات و بیوتکنولوژی. دانشکده کشاورزی. دانشگاه زابل. زابل. ایران | ||
| 2گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| 3پژوهشکده زیست فناوری کشاورزی، دانشگاه زابل، زابل، ایران | ||
| چکیده | ||
| با توجه به میزان اندک ترکیبات زیست فعال مهم، مانند گلیسیریزین و گلیسریتنیک اسید در شیرین بیان و پیچیدگی سنتز شیمیایی آنها، تولید درون شیشه ای‌ریشه موئین و تحریک تولید آنها با استفاده از الیسیتورها روشی کارآمد برای تولید در شرایط درون شیشه‌ای می‌باشد. لذا در این پژوهش، با استفاده از اگروباکتریوم رایزوژنز اقدام به تولید ریشه موئین شد. جهت حذف باکتری، بعد از تلقیح ازدو نوع آنتی بیوتیک سفوتاکسیم معمولی و نمک سفوتاکسیم سدیم، و برای افزایش تولید ترکیبات زیست فعال از الیسیتور متیل جاسمونات با غلظت 100 میکرومولار استفاده شد. برداشت ریشه‌ها در یک، دو، سه و پنج روز بعد از تیمار انجام شد. نتایج نشان دادکه محتوای گلیسیریزین، گلیسریتنیک اسید و فلاونوئید کل در تمام تیمارها نسبت به تیمار شاهد کاهش داشت. میزان این کاهش در تیمار بدون الیسیتور ، حذف باکتری با یک واکشت با نمک سفوتاکسیم سدیم بیشترین (959/0 و 925/0 برابر به‌ترتیب برای گلیسیریزین و گلیسریتنیک اسید) بود. میزان فنل کل (22/31 گالیک اسید بر گرم وزن خشک) و فعالیت آنتی اکسیدانی (85/90 درصد)، در ریشه‌هایی که دو روز تحت تاثیر الیسیتور بودند و و ریشه‌های شاهد بیشترین مقدار بود. در این تحقیق، میزان گلیسیریزین و گلیسریتنیک اسید تحت تاثیر الیسیتور متیل جاسمونات قرار نگرفت و یا کاهش یافت که می‌تواند به اثر گونه و اکوتیپ، تعداد واکشت بعد از تلقیح، نوع آنتی بیوتیک، غلظت الیسیتور و مدت زمانی که ریشه‌های موئین تحت تاثیر الیسیتور قرار گرفته‌اند نسبت داده شود. بر اساس نتایج، استفاده از سفوتاکسیم برای حذف باکتری از محیط کشت توصیه می‌شود. | ||
| کلیدواژه‌ها | ||
| گلیسیریزین؛ گلیسریتنیک اسید. نمک سفوتاکسیم سدیم | ||
| عنوان مقاله [English] | ||
| Effect of Methyl Jasmonate on the Production of Bioactive Compounds in Hairy Root Licorice (Glycyrrhiza glabra) | ||
| نویسندگان [English] | ||
| Maryam Allahdou1؛ Leila Mehravaran2؛ Hamideh Khajeh3 | ||
| 1Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran | ||
| 2Department of Plant Breeding and Biotechnology, Agriculture Faculty, University of Zabol, Zabol. Iran | ||
| 3Institute of Agricultural Biotechnology, University of Zabol, Zabol. Iran | ||
| چکیده [English] | ||
| Considering the low amount of important bioactive compounds such as glycyrrhizin and glycyrrhetinic acid in licorice and their complex biosynthesis, in vitro production of hairy roots is an efficient method for licoric secondary metabolites production. Therefore, this experiment aimed to investigate the efficiency of licorice hairy roots in the presence of Agrobacterium rhizogenes and the effect of methyl jasmonate (MeJA) at 100 µm, as an elicitor, on inducing glycyrrhizin and glycyrrhetinic acid production.. After inoculation, two kinds of the cefotaxime were used to eliminate the bacterium. The hairy roots were then harvested after one, two, three, and five days of elicitation. Overall, The results showed that the amount of glycyrrhizin, glycyrrhetinic acid and total flavonoids decreased in all treatments compared to the control, and the magnitude of this reduction in hairy roots treated with one inoculation of cefotaxime sodium salt and no MeJA was the highest in terms of glycyrrhizin (0.959 times) and glycyrrhetinic acid (0.925 times) production. In addition, the amount of total phenol (31.22 GAE/g D.W) and antioxidant activity (90.85%) was highest in in the hairy roots subjected to MeJA for two days and the control. MeJA had negative or no effect on production of glycyrrhizin and glycyrrhetinic acid, which can be related to type of species, ecotype, and antibiotics as well as the number of subcultures after inoculation, the concentration of the elicitor, and a period of time at which hairy roots exposed to a given elicitor. Based on our findings, cefotaxime is recommended to omit the bacteria from culture media. Overall, our findings could be used as an economically method to increase bioactive compounds, especially glycyrrhizin and glycerethnic acid, at a short time and low cost. | ||
| کلیدواژه‌ها [English] | ||
| Cefotaxime sodium salt, Glycyrrhetinic acid, Glycyrrhizin, Methyl Jasmonate | ||
| مراجع | ||
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