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مجله علوم کشاورزی ایران | ||
Article 5, Volume 32, Issue 1 - Serial Number 905, March 2001 PDF (549.95 K) | ||
Abstract | ||
Investigations on sugar beet mosaic, through different antisera methods used, showed that sugar beet mosaic virus (BMY) and cucumber mosaic virus (CMY) caused the disease in sugar beet. in Karaj region. The viruses were purified using differential centrifugation. The virus yields in the final preparations were 0.35 mg/mL for BMV and 0.98 mg/ml. for CMY , absorption spectrum of them were 260-240 and 260-220 nanometers respectively. Antisera against the above mentioned viruses were prepared in rabbit then titrated. Titration for BMV and CMY were 256 and 512 respectively in micropredpitin tests. Research in distribution of the isolated viruses showed that BMY ( 414 from 427 samples) was more widely spread thanCMY (13 from 427 samples) in all areas, Because of wider distribution and spread of BMY than the othc_r, its effect on seed production, ability to germinate and seed bearing pote:ntial were studied. The results indicated considerable reduction in seed production, but no effect on germination or seed bearing ability. | ||
Keywords | ||
Beet mosaic virus; Concentrate; Cucumber mosiac virus; purification; Sugarbeet | ||
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