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مجله علوم کشاورزی ایران
Article 14, Volume 4, Issue 0 - Serial Number 1031, June 1999 PDF (507.69 K)
Abstract
Yellow rust in wheat occurs annually in most wheat-growing areas in Iran. The identification of molecular markers linked to yellow rust resistance has the potential to improve the efficiency of selection in wheat breeding programs. DNA Amplification Fingerprinting (DAF) technology was used with the objective of identifying DNA markers linked to the yellow rust resistance. Two bulks were made by pooling extracted DNA, each bulk (one resistant and one susceptible) being resulted from an F2 population obtained from a cross between a susceptible and a resistant parent. The bulks were evaluated with many random primers. One primer produced a band which was associated with the traget gene Yr5.
Keywords
BSA; Linkage; Marker-assisted selection; Molecular marker; PCR; Polymorphism; RCR; Resistance gene; Wheat; yellow rust

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