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بررسی آلودگی به ویروس IHN در مزارع پرورش ماهی قزل آلای رنگین کمان (On corhynchus mykiss) در دو استان تهران و چهار محال و بختیاری با استفاده از تکنیک آنتی بادی درخشان به روش غیر مستقیم | ||
| مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
| مقاله 4، دوره 61، شماره 1 - شماره پیاپی 822096، فروردین 1385، صفحه 19-22 اصل مقاله (3.22 M) | ||
| نویسندگان | ||
| روزبه فلاحی1؛ مهدی سلطانی2؛ سید جلیل ذریه زهرا3؛ فرهید همت زاده4 | ||
| 1موسسه تحقیقات واکسن و سرم سازی رازی حصارک | ||
| 2گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، دانشگاه تهران | ||
| 3موسسه تحقیقات شیلات ایران | ||
| 4گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران | ||
| چکیده | ||
| Objective: Serological diagnosis of infectious haematopoietic necrosis virus (IHNV) in tissues of kidney, spleen and liver of rainbow trofi(O.mykiss)fry. Design: Cross- sectional study. Animals:Ninty nine samples consisting of smears from kidney, spleen and liver were obtained from rainbow trout. Procedure: Kidney, spleen and liver from rainbow trout (O.mykiss) fry less than 3 g body weight obtained from some farmed rainbow trout of Tehran and Char mahal - va- Bakhtiari provinces. Indirect immunofluorescent antibody test (IFAT) was undertaken using both polyclonal and monoclonal antibodies to IHNV to identify the IHNV antigens in the tissue samples. Results: Only eleven samples from the region of Char mahal - va - Bakhtiari were positive for IHNV using both monoclonal and polyclonal antibodies to IHNV.Conclusion: Because of economic losses due to disease caused by IHNV in farmed rainbow trout , it is necessary to do a comprehensive screening test, virus isolation and evaluation of the pathogenicity of recovered virus. | ||
| عنوان مقاله [English] | ||
| Serological Diagnosis of Infectious Haematopoietic Necrosis Disease (IHN) in Rainbow Trout (Oncorhynchus mykiss) using Indirect Fluorescent Antibody Test | ||
| نویسندگان [English] | ||
| Roozbeh Fallahi1؛ Mahdi Soltani2؛ Seyed Jalil Zorriehzahra3؛ Farhid Hemmatzadeh4 | ||
| 1Unit of Aquatic Animal Health and Diseases, RaTi Vaccine and SerumRe search Institute, Karaj - I ran | ||
| چکیده [English] | ||
| Objective: Serological diagnosis of infectious haematopoietic necrosis virus (IHNV) in tissues of kidney, spleen and liver of rainbow trofi(O.mykiss)fry. Design: Cross- sectional study. Animals:Ninty nine samples consisting of smears from kidney, spleen and liver were obtained from rainbow trout. Procedure: Kidney, spleen and liver from rainbow trout (O.mykiss) fry less than 3 g body weight obtained from some farmed rainbow trout of Tehran and Char mahal - va- Bakhtiari provinces. Indirect immunofluorescent antibody test (IFAT) was undertaken using both polyclonal and monoclonal antibodies to IHNV to identify the IHNV antigens in the tissue samples. Results: Only eleven samples from the region of Char mahal - va - Bakhtiari were positive for IHNV using both monoclonal and polyclonal antibodies to IHNV.Conclusion: Because of economic losses due to disease caused by IHNV in farmed rainbow trout , it is necessary to do a comprehensive screening test, virus isolation and evaluation of the pathogenicity of recovered virus. | ||
| کلیدواژه‌ها [English] | ||
| IFAI, IHNV, Rainbow trout | ||
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