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Mojtahedi, Narges, Koobaz, Parisa, Fathi, Mohammad, Dabirashrafi, Omid, Azadi, Pejman, Khosravi, Solmaz. (1393). Maturating, Enlarging and Breaking Dormancy of In Vitro Lilium Bulblets. , 1(2), 101-109. doi: 10.22059/ijhst.2014.52779
Narges Mojtahedi; Parisa Koobaz; Mohammad Fathi; Omid Dabirashrafi; Pejman Azadi; Solmaz Khosravi. "Maturating, Enlarging and Breaking Dormancy of In Vitro Lilium Bulblets". , 1, 2, 1393, 101-109. doi: 10.22059/ijhst.2014.52779
Mojtahedi, Narges, Koobaz, Parisa, Fathi, Mohammad, Dabirashrafi, Omid, Azadi, Pejman, Khosravi, Solmaz. (1393). 'Maturating, Enlarging and Breaking Dormancy of In Vitro Lilium Bulblets', , 1(2), pp. 101-109. doi: 10.22059/ijhst.2014.52779
Mojtahedi, Narges, Koobaz, Parisa, Fathi, Mohammad, Dabirashrafi, Omid, Azadi, Pejman, Khosravi, Solmaz. Maturating, Enlarging and Breaking Dormancy of In Vitro Lilium Bulblets. , 1393; 1(2): 101-109. doi: 10.22059/ijhst.2014.52779

Maturating, Enlarging and Breaking Dormancy of In Vitro Lilium Bulblets

International Journal of Horticultural Science and Technology
مقاله 2، دوره 1، شماره 2، اسفند 2014، صفحه 101-109    اصل مقاله (743.85 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2014.52779
نویسندگان
Narges Mojtahedi* 1؛ Parisa Koobaz2؛ Mohammad Fathi2؛ Omid Dabirashrafi2؛ Pejman Azadi2؛ Solmaz Khosravi2
1Agriculture Biotechnology Research Institute of Iran (ABRII) Seed and Plant Improvement Institutes Campus Mahdasht Road P. O. Box 31535-1897 Karaj, Iran
2Agriculture Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
چکیده
One of the most effective ways to propagate Lilium is by using tissue culture techniques, preferably through bulblet production. In addition to the surviving percentage of Lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing Lilium micropropagation. Different concentrations of sucrose or sorbitol as carbohydrate sources were investigated as maturation and enlargement factors on in vitro bulblets of Lilium longiflorum cv. „Ceb-dazzle‟. Five concentrations of gibberellic acid and 4°C cold treatment for 6, 7, 8, 9, 10 and 11 weeks in the greenhouse were studied in relation to the dormancy breaking of three in vitro Lilium bulblets cultivars: „Simplon‟, „Navona‟ and „Ceb-dazzle‟, which belong to the Oriental, Asiatic and LA hybrids. The results showed that 3% of sucrose and two weeks of culture significantly increased the bulblet maturation and enlargement of „Ceb-dazzle‟. One mg L-1 gibberellic acid for six weeks, or cold treatment for six to eight weeks, had significant positive effects on the dormancy breaking of in vitro bulblets of Lilium cultivars.
کلیدواژه‌ها
Carbohydrate sources؛ Cold treatment؛ Commercial bulb؛ Micropropagation
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