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کاربرد سیلیکون در القای مقاومت سیستمیک علیه بیماری بلایت فوزاریومی خوشة گندم | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 5، دوره 3، شماره 2، مهر 1393، صفحه 127-137 اصل مقاله (644.23 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2014.53673 | ||
| نویسندگان | ||
| وحید قاضی محسنی1؛ سید کاظم صباغ* 2؛ صدیقه اسمعیل زاده بهابادی3؛ مرتضی قربانی4 | ||
| 1دانشجوی کارشناسی ارشد، بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکدة کشاورزی، دانشگاه زابل | ||
| 2استادیار، بیوتکنولوژی گیاهی، گروه گیاه‌پزشکی و پژوهشکدة زیست فناوری گیاهی، دانشگاه زابل | ||
| 3استادیار، گروه زیست‌شناسی، دانشکدة علوم پایه، دانشگاه زابل | ||
| 4استادیار، بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه زابل | ||
| چکیده | ||
| در این تحقیق اثر غلظت‌های مختلف سیلیکون (صفر، 2، 4 و 6 میلی‌مولار) بر بیان چند ژن مرتبط با بیماریزایی و فعالیت تعدادی آنزیم آنتی‌اکسیدان مطالعه شد. گیاهان در مرحلة خوشه‌دهی از طریق محلول خاک تیمار شدند. سپس، گیاهان تیمار‌شده با 1 میلی‌لیتر سوسپانسیون اسپور قارچ حاوی 106 ماکروکنیدی به روش تزریق در زیر محل خوشه، مایه‌زنی شدند و در بازه‌های زمانی صفر، 24، 72 و 120 ساعت پس از تلقیح، برداشت نمونه برای اندازه‌گیری فعالیت آنزیمی و بیان ژن انجام شد. نتایج نشان داد که شدت بیماری در گیاهان تیمار‌شده در مقایسه با شاهد کاهش داشت. آنالیز بیوشیمیایی نشان داد که فعالیت دو آنزیم پراکسیداز و پلی‌فنل اکسیداز در تمام غلظت‌ها افزایش یافت. نتایج آنالیز مولکولی نشان از افزایش سطح بیان ژن‌های بتا ـ 1 و 3 گلوکاناز و اگزالات اکسیداز در گیاهان تحریک‌شده با سیلیکون در غلظت 6 میلی‌مولار در بازة زمانی 120 ساعت بعد از مایه‌زنی داشت. به‌طور کلی، نتایج این تحقیق نشان می‌دهد که سیلیکون می‌تواند مقاومت گیاهان در برابر عوامل بیماریزای قارچی را از طریق مقاومت اکتسابی سیستمیک افزایش دهد. | ||
| کلیدواژه‌ها | ||
| بیان ژن؛ سیلیکون؛ فوزاریوز؛ .qRT-PCR | ||
| عنوان مقاله [English] | ||
| The use of silicon on systemic resistance induction against fusarium head blight disease of wheat | ||
| نویسندگان [English] | ||
| Vahid Ghazimohseni11؛ Seyed Kazem Sabbagh2؛ sedigheh Esmaeilzadeh Bahabadi3؛ Morteza Ghorbani4 | ||
| 1MSc student of plant pathology, department of plant protection, University of Zabol, Iran | ||
| 2Assistant professor,department of plant pathology, and institute of plant biotechnology, University of Zabol, Iran | ||
| 3Assistant professor, department of biology, faculty of Basic Sciences, University of Zabol, Iran | ||
| 4Assistant professor, faculty of plant pathology, University of Zabol, Iran | ||
| چکیده [English] | ||
| In this study the effect of different concentrations of silicon( 0, 2 , 4 and 6 mM, on expression of some genes involved in resistance and activity of several antioxidan enzymes was investigated: The plants was treated in flowering stage via soil solution. The treated plants were inoculated with 106 spore suspended in 1 mL by injection into the lower part of the spikelets and then were harvested at 0, 24, 72 and 120 h after inoculation for meausering enzymatic activity of gene expression analysis. The results showed that disease severity was reduced in treated plants compared to the control. Biochemical analysis showed that activity of peroxidase and polyphenol oxidase enzymes at all concentrations of silicon was increased significantly copared to the control . Gene expression analysis showed that expression level of beta-1, 3-glucanase and oxalate oxidase genes induced plants with silicon concentration of 6mmol/l were increased at 120h time point after inoculation. Overal, The results of this research indicate that silicon could increase plant resistance against pathogenic fungi by induction of induced systemic resistance. | ||
| کلیدواژه‌ها [English] | ||
| Fusariuse, Silicone, qRT-PCR, gene expression | ||
| مراجع | ||
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