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مقایسة اثر محیط‌های کشت آلی روی میزان جمعیت تیپ وحشی و تیپ جهش‌یافتة باکتری بیوکنترل Pseudomonas fluorescens VUPf5 | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 6، دوره 3، شماره 2، مهر 1393، صفحه 139-150 اصل مقاله (510.24 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2014.53674 | ||
| نویسندگان | ||
| آرزو لگزیان* ؛ روح اله صابری‌ریسه؛ پژمان خدایگان | ||
| دانشجویان سابق کارشناسی ارشد، استادیاران بیماری‌شناسی گیاهی، دانشکدة کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان | ||
| چکیده | ||
| در این تحقیق رشد دو سویة باکتریPseudomonas fluorescens VUPf5 و fluorescens VUPf5-1 P. (باکتری جهش‌یافتة مشابه جهش‌یافتة سیستم تنظیمی gac) در محیط‌های کشت آلی ارزیابی شد. این جهش خودبه‌خودی تولید ترکیبات ضد قارچی سیانید هیدروژن، آنتی‌بیوتیک فنازین و آنزیم پروتئاز را در سویة بیوکنترل Pseudomonas fluorescens VUPf5 متوقف کرد. با استفاده از محیط‌های کشت آلی همراه یا بدون سولفات روی سعی شد که جمعیت جهش‌یافته‌ها تا حد امکان کاهش یابد. با هدف افزایش رشد جمعیت VUPf5 و کاهش رشد جمعیت VUPf5-1، شش منبع کربنی (ملاس چغندرقند، نشاسته، سیب‌زمینی، خرما، شکر، سیب) ،سه منبع ازتی (سویا، اوره، سولفات آمونیوم) و یک منبع کربنی ـ ازتی (جوانة گندم) بررسی شدند. محیط کشت 13B (ملاس چغندرقند، جوانة گندم، سویا، شکر، سیب و سولفات منگنز)، محیط کشت بهتری از لحاظ جمعیت باکتری VUPf5 (1010× 56/5 سلول باکتری در هر میلی‌لیتر) در مقایسه با محیط‌های کشت دیگر شناخته شد و اختلاف جمعیت باکتری در مقایسه با سایر محیط‌ها معنی‌دار بود. محیط کشت 1D (ملاس چغندرقند، جوانة گندم، سویا، شکر، سیب و سولفات روی) سبب کاهش چشمگیری در تکثیر پرگنه‌های جهش‌یافته شد. نتایج به‌دست‌آمده می‌تواند مبنای قابل اعتمادی برای تکثیر بهینة عامل بیوکنترل در فرایند‌های فرمانتاسیون را فراهم کند. | ||
| کلیدواژه‌ها | ||
| بهینه‌سازی؛ تکثیر؛ جهش‌یافته؛ سودوموناس | ||
| عنوان مقاله [English] | ||
| Comparison of effect of organic media on wild-type and mutation-type population of biocontrol bacterium Pseudomonas fluorescens VUPf5 | ||
| نویسندگان [English] | ||
| Arezoo Lagzian؛ Roohallah Saberi-Riseh؛ Pejman Khodaygan | ||
| Former Graduate Student and Assistant Professors of Plant Pathology, College of Agriculture, Vali-E-Asr University of Rafsanjan | ||
| چکیده [English] | ||
| In this study, the growth of two strains Pseudomonas fluorescens VUPf5 and P. fluorescens VUPf5-1 (mutant bacterium similar to mutants of regulatory GacS/GacA system) were evaluated in the organic media. This spontaneous mutation blocked biosynthesis of the antimicrobial compounds hydrogen cyanide, phenazine and exoprotease by biocontrol strain Pseudomonas fluorescens VUPf5. By using organic media amendments by zinc sulfate or without it considered to reduce the growth of mutants. With the aim of increasing VUPf5 population and decreasing VUPf5-1 population six carbon sources (sugar beet molasses, starch, potato, date, sugar and apple), three nitrogen sources (soybean, urea, ammonium sulfate) and one nitrogen-carbon source (wheat germ) were assessed. Medium 13B (molasses, wheat germ, soybean, sugar, apple, manganese sulfate) was more effective for growth of strain VUPf5 (5.56× 1010 cells per ml) compared other treatments and population difference was significant. Medium 1D (molasses, wheat germ, soybean, sugar, apple, zinc sulfate) greatly caused to reproduction reduction in strain VUPf5-1. The results could provide a reliable basis for optimized mass multiplication of biocontrol agent in fermentation processes. | ||
| کلیدواژه‌ها [English] | ||
| Mutant, Optimization, proliferation, Pseudomonas | ||
| مراجع | ||
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