|تعداد مشاهده مقاله||107,936,430|
|تعداد دریافت فایل اصل مقاله||84,358,060|
Determination of aflatoxin M1 levels in raw milk samples using ELISA and high-performance liquid chromatography in Qazvin, Iran
|Journal of Mycology Research|
|مقاله 7، دوره 2، شماره 1، خرداد 2015، صفحه 41-48 اصل مقاله (356.77 K)|
|نوع مقاله: Original Articles|
|Reza Norian1؛ Razzagh Mahmoudi2؛ Alireza Porfarzaneh3؛ Faramarz Mashatian3؛ Ata Kaboudari1؛ Seyedeh Faezeh Rahimi Pir Mahalleh1؛ Farzad Katiraee* 4|
|1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran|
|2Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran|
|3Laboratory of Food Quality Control and Hygiene, Veterinary Medicine Office, Qazvin, Iran|
|4Department of Pathobiology, Division of Mycology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran|
|Use of raw milk and dairy products containing aflatoxin M1 (AFM1) has led to concern in|
consumers. The present study determined the AFM1 in raw cow's milk in Qazvin
province. In this research, 170 raw cow's milk were collected from dairy farm, dairy
factories, milk collection centers, and milk supply centers in Qazvin province during cold
seasons in 2013, and all samples were examined for AFM1. The samples were analyzed
with a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit and
high-performance liquid chromatography (HPLC). AFM1 contamination was observed in
all milk samples. Fifty-seven milk samples (33.52%) had a contamination of AFM1,
higher than the threshold level of The Institute of Standards and Industrial Research of
Iran (0.5 ng/ml), whereas in 113 milk samples (66.48%), concentration of AFM1 was less
than the limits permitted. The mean concentration of AFM1 in dairy farm was 0.215
ng/ml, in dairy factories 0.268 ng/ml, in milk collection centers 0.734 ng/ml, and in milk
supply centers 0.409 ng/ml. Because of high levels of contamination observed in samples,
regular monitoring of contamination in milk samples and controlling most contaminating
causes are necessary.
|aflatoxin M1؛ ELISA؛ HPLC؛ Milk|
1. Aycicek, H., Aksoy, A., Saygi, S., 2005. Determination of aflatoxin levels in some dairy and food products which consumed in Ankara, Turkey. Food Control, 16: 263-266.
2. Bakirci, I., 2001. A study on the occurrence of aflatoxin M1 in milk and milk products produced in Van province of Turkey. Food Control,12: 47-51.
3. Beheshti, H.R., Asadi, M., 2014. Aflatoxins in animal feed in Iran. Food Additives & Contaminants: Part B, 7: 40-42.
4. Eskandari, M. H., Pakfetrat, S., 2014.
Aflatoxins and heavy metals in animal feed in Iran. Food Additives & Contaminants: Part B, 7: 202-207.
5. D'Mello, J.P.F., Macdonald, A.M.C., 1997. Mycotoxins. Animal Feed Science and Technology, 69: 155-166.
6. Gürbay, A., Aydın, S., Girgin, G., Engin, A.B., Şahin, G., 2006. Assessment of aflatoxin M1 levels in milk in Ankara, Turkey. Food Control, 17: 1-4.
7. Ivastava, V.P., Bu-Abbas, A., Al-Johar, W., Al-Mufti, S., Siddiqui, M.K.J., 2001. Aflatoxin M 1 contamination in commercial samples of milk and dairy products in Kuwait. Food Additives & Contaminants, 18: 993-997.
8. Kamkar, A., 2005. A study on the occurrence of aflatoxin M1 in raw milk produced in Sarab city of Iran. Food Control, 16: 593-599.
9. Kangethe, E. K., Langa, K. A. 2009. Aflatoxin B1 and M1 contamination of animal feeds and milk from urban centers in Kenya. African Health Sciences, 9: 218-226.
10.Lopez, C., Ramos, L., Ramadan, S., Bulacio, L., Perez, J., 2001. Distribution of aflatoxin M1 in cheese obtained from milk artificially contaminated. International journal of food microbiology, 64: 211-215.
11.Mahmoudi, R., Norian, R., Katiraee, F., Pajohi Alamoti, M.R., 2013. Total aflatoxin contamination of maize produced in different regions of Qazvin-Iran. International Food Research Journal, 20(5): 2901-2904.
12.Markaki, P., Melissari, E., 1997. Occurrence of aflatoxin M1 in commercial pasteurized milk determined with ELISA and HPLC. Food Additives & Contaminants, 14: 451-456.
13.Martins, M.L., Amp, X., Martins, H.M.,
2004. Aflatoxin M1 in yoghurts in Portugal. International Journal of food microbiology, 91: 315-317.
14.Park, D., 2002. Effect of Processing on Aflatoxin, In: DeVries, J., Trucksess, M., Jackson, L., Mycotoxins and Food Safety, Springer Press, UK.173-179.
15.Rahimi, E., Kargar, A.R., Zamani, F. 2008. Assessment of aflatoxin B1 levels in animal feed of dairy farms in Chaharmahal & Bakhtiari. Animal Science Journal (Pajouhsh & Sazandegi), 79: 66-71.
16.Rashid, N., Bajwa, M., Rafeeq, M., Khan, M., Ahmad, Z., Tariq, M., Wadood, A., Abbas, F. 2012. Prevalence of aflatoxin B1 in finished commercial broiler feed from west central Pakistan. Journal of Animal and Plant Sciences, 22: 6-10.
17.Rastogi, S., Dwivedi, P.D., Khanna, S.K., Das, M., 2004. Detection of Aflatoxin M1 contamination in milk and infant milk products from Indian markets by ELISA. Food Control, 15: 287-290.
18.Rodríguez Velasco, M.L., Calonge Delso, M.M., OrdónezEscudero, D., 2003. ELISA and HPLC determination of the occurrence of aflatoxin M 1 in raw cow's milk. Food Additives & Contaminants, 20: 276-280.
19.Samarajeewa, U., Sen, A.C., Cohen, M.D., Wei, C.I., 1990. Detoxification of Aflatoxins in Foods and Feeds by Physical and Chemical Methods. Journal of Food Protection, 53: 489-501.
20.Van Egmond, H.P., 1983. Mycotoxins in dairy products. Food Chemistry, 11: 289-307.
21.Van Egmond, H.P., 1993. Rationale for regulatory programmes for mycotoxins in human foods and animal feeds. Food Additives & Contaminants, 10: 29-36.
22.Van Egmond, H.P., 1995. Mycotoxins: Regulations, quality assurance and
reference materials. Food Additives & Contaminants, 12: 321-330.
23.Vagef, R., Mahmoudi, R., 2013. Occurrence of Aflatoxin M1 in raw and pasteurized milk produced in west region of
Iran (during summer and winter). International Food Research Journal, 20(3): 1421-1425.
تعداد مشاهده مقاله: 2,511
تعداد دریافت فایل اصل مقاله: 1,937