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تأثیر احساس برخی الگو‌های مولکولی مرتبط با میکروب شناخته‌شده در افزایش مقاومت گیاه آرابیدوپسیس | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 3، دوره 4، شماره 1، فروردین 1394، صفحه 13-22 اصل مقاله (508.14 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2015.54511 | ||
| نویسندگان | ||
| وحید فلاح زاده ممقانی1؛ مسعود احمدزاده* 2؛ کیوان بهبودی3 | ||
| 1استادیار گروه گیاه‌پزشکی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران | ||
| 2استاد گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| 3دانشیار گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| چکیده | ||
| الگوی‌های مولکولی مرتبط با میکروب (Microbe-associated molecular patterns-MAMPs) نقش کلیدی در فعال‌سازی پاسخ‌های ایمنی ذاتی (Innate immunity) در جانوران و به‌صورت مشابهی، به‌عنوان انگیزنده‌های (Elicitors) پاسخ‌های دفاعی در گیاهان دارند. با وجود این، بررسی‌های زیادی دربارة اهمیت واقعی آن‌ها در مقاومت گیاه نسبت به بیمارگرها وجود ندارد. در این تحقیق اثر مشتقات فاکتور نسخه‌برداری Ef-Tu و فلاژلین به‌عنوان MAMPهای شناخته‌شده، در افزایش مقاومت گیاه آرابیدوپسیس نسبت به بیمارگرهای مهم قارچی و باکتریایی بررسی شد. پیش‌تیمار گیاهان Col-0 با هر دوMAMP به‌صورت معنی‌داری مقاومت گیاهان نسبت به قارچ‌های Botrytis cinerea و Alternaria brassicicola را افزایش داد. پیش‌تیمار برگ‌های Col-0 با هر دو MAMP، منحنی رشد باکتری Pseudomonas syringae pv. tomato (Pst DC3000) را به‌صورت معنی‌داری تحت تأثیر قرار داد و باعث کاهش سرعت رشد آن شد. علاوه بر این، جمعیت باکتری Xanthomonas axonopodis pv. citri (Xac306) که به‌عنوان غیربیماریزا روی آرابیدوپسیس مطرح است، در برگ‌های گیاهان وحشی که با MAMPها تیمار نشده بودند، بدون تغییر باقی ماند و 48 ساعت بعد از تلقیح، نه کاهش و نه افزایش نشان داد. هر‌چند پیش‌تیمار برگ‌های این گیاه با elf18 به کاهش جمعیت باکتری منجر شد. نتایج این تحقیق نقش ایمنی ذاتی در افزایش مقاومت گیاه نسبت به بیمارگرها را نشان داد. | ||
| کلیدواژه‌ها | ||
| آرابیدوپسیس؛ ایمنی ذاتی؛ MAMP؛ Pseudomonas؛ Xanthomonas؛ Botrytis؛ Alternaria | ||
| عنوان مقاله [English] | ||
| Increased disease resistance in Arabidopsis thaliana through perception of well known pathogen-associated molecular patterns | ||
| نویسندگان [English] | ||
| Vahid Fallahzadeh-Mamaghani1؛ Masoud Ahmadzadeh2؛ Keivan Behboudi3 | ||
| 1Assistant professor, Department of plant protection, Azarbaijan Shahid Madani Uniersity, Tabriz, Iran | ||
| 2Professor, Department of plant protection, University College of Agriculture and natural science, Tehran University | ||
| 3Associate professor, Department of plant protection, University College of Agriculture and natural science, Tehran University | ||
| چکیده [English] | ||
| Microbe-associated molecular patterns (MAMPs) play key roles as activators of the innate immune response in animals and, analogously, as elicitors of defense responses in plants. However, there are not much investigations concerning their real importance in plant resistance against pathogens. Here we used derivatives of flagellin and Ef-Tu as two well-known MAMPs against some important fungal and bacterial pathogens on Arabidopsis thaliana. Pretreatment of Col-0 plants with both MAMPs, significantly increased the resistance of plants against B. cinerea and A. brassicicola. Growth curve of Pseudomonas syringaepv. tomato (Pst DC3000) in Arabidopsis leaves was significantly influenced by both of the MAMPs. Moreover Xanthomonas axonopodis pv. citri (Xac 306) that is a non-pathogenic bacteria on Arabidopsis, neither grow nor decline in Col-0 leaves for 48 hours after inoculation. However the number of bacteria in the plants pretreated with elf18 was significantly decreased. Results of this study clearly showed the role of innate immunity in plant resistance against pathogens. | ||
| کلیدواژه‌ها [English] | ||
| innate immunity, Arabidopsis, Pseudomonas, MAMP | ||
| مراجع | ||
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