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تنوع ژنتیکی و ساختار جمعیتی سخت پوست Paramysis intermedia در مناطق تیاب و خلیج گواتر با استفاده از توالی یابی ژن میتوکندریایی 16SrRNA | ||
| شیلات | ||
| مقاله 13، دوره 68، شماره 2، تیر 1394، صفحه 329-342 اصل مقاله (682.83 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2015.55123 | ||
| نویسندگان | ||
| سمیرا وحیدی نژاد1؛ ایمان سوری نژاد* 2؛ سعید تمدنی جهرمی3؛ آرش اکبرزاده2؛ فریبا کشاورزی1؛ فریدون چکمه دوز قاسمی4 | ||
| 1دانشجوی کارشناسی ارشد-گروه شیلات، دانشکدة علوم و فنون دریایی و جوی، دانشگاه هرمزگان | ||
| 2استادیار گروه شیلات، دانشکدة علوم و فنون دریایی و جوی، دانشگاه هرمزگان | ||
| 3استادیار بخش ژنتیک، پژوهشکدة اکولوژی خلیج فارس و دریای عمان، هرمزگان | ||
| 4کارشناس ارشد بخش ژنتیک و اصلاح نژاد، پژوهشکدة آبزی‌پروری آب‌های داخلی، بندر انزلی | ||
| چکیده | ||
| یکی از مهم‌ترین زئوپلانکتون‌هایی که در نواحی مصبی و دریایی مورد تغذیة آبزیان قرار می‌گیرد سخت‌پوست پارامایسیس اینترمدیا Paramysis intermedia است. با توجه به وجود ذخایر این زئوپلانکتون در خلیج فارس و دریای عمان، آگاهی از ساختار ژنتیکی آن به منظور حفظ تنوع زیستی و خزانة ژنی دارای اهمیت است. در پژوهش حاضر برای اولین‌بار ساختار ژنتیکی و جمعیتی سخت‌پوست پارامایسیس اینترمدیا در دو منطقة تیاب و خلیج گواتر با استفاده از توالی‌یابی ژن 16S rRNA میتوکندریایی بررسی شد. نتایج ده نمونة توالی‌یابی‌شده شامل 365 باز هم‌ردیف‌شدة ژن 16S rRNA، 339 جایگاه ژنی مونومورف، 26 جایگاه ژنی پلی‌مورف و 27 موتاسیون نشان‌دهندة نرخ بسیار کم موتاسیون در این گونه بین مناطق مورد مطالعه بود. هیچ گونه چندشکلی اضافه و حذف مشاهده نشد. تعداد نه هاپلوتیپ در دو منطقه به دست آمد و میزان تنوع هاپلوتیپی و تنوع نوکلئوتیدی برای تمامی نمونه‌ها بین دو منطقه به ترتیب 003/0 ± 978/0 و 000/0 ± 035/0 محاسبه شد. مقدار هتروزایگوسیتی مورد انتظار برای منطقة تیاب بیشتر (184/0 ± 287/0) از خلیج گواتر (088/0 ± 014/0) بود. مقدار Fst در سطح احتمال 05/0 بین دو جمعیت 18/0 و معنی‌دار بود و در ترسیم درخت فیلوژنتیکی این جدایی به‌وضوح دیده شد. میانگین مقدار آزمون Tajima's D و Fu's FS بین دو منطقه به ترتیب 12/0- و 51/2 و غیر معنی‌دار بود که نشان‌دهندة نبود بسط جمعیتی بین نمونه‌های دو منطقه است. نتایج نشان داد که سخت‌پوست پارامایسیس اینترمدیا در دو منطقة مطالعه‌شده، به خصوص منطقة تیاب، از تنوع ژنتیکی بالایی برخوردار است و هر یک جمعیت مستقلی دارند. | ||
| کلیدواژه‌ها | ||
| پارامایسیس اینترمدیا Paramysis intermedia؛ تعیین توالی؛ تیاب؛ خلیج گواتر؛ 16S rRNA | ||
| عنوان مقاله [English] | ||
| Genetic diversity and population structure of crustacean Paramysis intermedia in Tiab and Gwatr Bay regions using mitochondrial 16S rRNA gene sequencing | ||
| نویسندگان [English] | ||
| Samira Vahidinejad1؛ Iman Sourinejad2؛ Saeid Tamadoni Jahromi3؛ Arash Akbarzadeh2؛ Fariba Keshavarzi1؛ Fereidon Chakmehdouz Ghasemi4 | ||
| 1MSc. Fisheries Department, Faculty of Marine and Atmospheric Science and Technology, University of Hormozgan, Iran | ||
| 2Assistant Professor, Fisheries Department, Faculty of Marine and Atmospheric Science and Technology, University of Hormozgan, Iran | ||
| 3Assistant Professor, Genetics Department, Persian Gulf and Oman Sea Research Center, Hormozgan Province, Iran | ||
| 4MSc. Genetics Department, Inland Aquaculture Research Institute, Anzali Port, Iran | ||
| چکیده [English] | ||
| The crustacean Paramysis intermedia is one of the most important zooplanktons which is used as food by aquatic animals in estuaries and marine environments. Regarding the existence of this zooplankton stocks in the Persian Gulf and Oman Sea, knowledge on genetic structure of this species is necessary for conservation of its biological diversity and gene pool. In present study, genetic diversity and population structure of Paramysis intermedia collected from Tiab and Gwatr Bay regions was assessed for the first time by mitochondrial 16S rRNA sequencing. Analysis of 10 sequenced samples including 365 alligned base pairs of 16S rRNA yielded 339 monomorphic loci, 26 polymorphic loci and 27 mutations, indicating a very low ratio of mutation between the two studied regions in this species. No insertions and deletions were observed. Nine haplotypes were identified in these two regions and haplotype and nucleotide diversity were 0.978±0.003 and 0.035±0.000, respectively for all samples between regions. Expected heterozygosity for Tiab region was higher (0.287±0.164) than Gwatr Bay (0.014±0.088). Based on the F- statistic parameter, population genetic distance was 0.18 between samples of the regions and was significant (P<0.05). According to the analysis of phylogenetic tree, the separation between the samples of the two regions was obvious. Mean values of Tajima’s D and Fu’s Fs between the two regions were -0.12 and 2.51, respectively. No significant values of these tests are indicative of no population expansion between the two regions of Tiab and Gwatr Bay. According to the results of this study, Paramysis intermedia populations of these regions have high genetic diversity especially in Tiab and each of its two populations is a genetically differentiated comunity. | ||
| کلیدواژه‌ها [English] | ||
| Gwatr bay, Paramysis intermedia, sequencing, Tiab, 16S rRNA | ||
| مراجع | ||
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