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ااثرات ترکیبی مخمر ساکارومایسس سرویزیا و آسپرژیلوس نایجر بر پارامترهای هماتولوژی و بیوشیمیایی سرم خون فیل ماهیان جوان پرورشی (Huso huso) | ||
مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
مقاله 15، دوره 70، شماره 4، دی 1394، صفحه 463-473 اصل مقاله (925.5 K) | ||
شناسه دیجیتال (DOI): 10.22059/jvr.2016.56468 | ||
نویسندگان | ||
احمد حسن پور فتاحی* 1؛ حجت الله جعفریان1؛ علیرضا خسروی2 | ||
1گروه شیلات، دانشگاه گنبد کاووس، گلستان- ایران | ||
2گروه قارچ شناسی، مرکز تحقیقات قارچ شناسی دانشکده دامپزشکی، دانشگاه تهران، تهران- ایران | ||
چکیده | ||
زمینه مطالعه: امروزه پروبیوتیکها به عنوان مکملهای غذایی میکروبی جایگزینی مناسب برای آنتیبیوتیکها تعریف میشوند که میتوانند شاخصهای سلامتی میزبان را تحت تأثیر قرار دهند. هدف: این تحقیق اثرات ترکیبی مخمر ساکارومایسس سرویزیا و آسپرژیلوس نایجر را بر پارامترهای هماتولوژی و بیوشیمیایی سرم خون فیل ماهیان جوان مورد بررسی قرار داد. روش کار: این مطالعه در قالب یک طرح کاملاًً تصادفی با 4 تیمار و 3 تکرار بر روی بچه فیل ماهیان پرورشی با میانگین وزنی (± خطای استاندارد) g81/2±8/31 انجام شد. بچه ماهیان به صورت تصادفی در 12 حوضچه فایبرگلاس با تراکم 30 قطعه ماهی در هر تانک توزیع گشته و با جیرههای غذایی حاوی (Cells/g) 106×2 در تیمار اول، (Cells/g) 106×4 در تیمار دوم، (Cells/g) 106×6 در تیمار سوم و جیره پایه بدون پروبیوتیک در گروه شاهد به مدت 8 هفته تغذیه شدند. نتایج: جیره غذایی مکمل سازی شده با غلظت (Cells/g) 106×6 در تیمار سوم، بطور معنیداری پارامترهای بیوشیمایی سرم خون را بهبود داد (05/0>p)، با این حال پارامترهای هماتولوژی در تیمارهای تحت تأثیر جیرههای مکمل سازی شده با پروبیوتیک تفاوت معنیداری را با گروه شاهد نشان ندادند (05/0<p). بررسی نتایج بدست آمده از فاکتورهای رشد نیز حاکی از بهبود این شاخصها در تیمارهای آزمایشی تغذیه شده با پروبیوتیک در مقایسه با گروه شاهد بود. نتیجهگیرینهایی: یافتههای این پژوهش نشان داد که بکارگیری ترکیبی ساکارومایسس سرویزیا و آسپرژیلوس نایجر در سطوح مورد مطالعه میتواند موجب بهبود عملکرد برخی از پارامترهای بیوشیمیایی نظیر فاکتورهای متابولیتی، ایمنی، آنزیمها و الکترولیتهای سرم خون فیل ماهیان جوان شود. براساس نتایج بدست آمده پیشنهاد میشود که غلظت بکار رفته در تیمار سوم به عنوان یک محرک ایمنی برای فیل ماهیان جوان پرورشی بکار گرفته شود. | ||
کلیدواژهها | ||
آسپرژیلوس نایجر؛ سرم خون؛ هماتولوژی؛ بچه فیل ماهی؛ ساکارومایسس سرویزیا | ||
عنوان مقاله [English] | ||
The combined effects of the yeast Saccharomyces cerevisiae and Aspergillus niger on the haematological and biochemical parameters of cultured juvenile beluga (Huso huso) | ||
نویسندگان [English] | ||
Ahmad Hasanpour Fattahi1؛ Hojatollah Jafaryan1؛ Alireza Khosravi2 | ||
1Department of Fishery, Gonbad University, Golestan- Iran | ||
2Mycology Research Center, Faculty of Veterinary, University of Tehran, Tehran- Iran | ||
چکیده [English] | ||
BACKGROUND: Probiotics, in form of microbial supplements, are known to be a suitable alternative for antibiotics and can affect the health indicators of host. Objectives: The present study was conducted to assess the combined effects of dietary autochthonous Saccharomyces cerevisiae and Aspergillus niger on haematological and serumbiochemicalparameters of beluga sturgeon (Huso huso) juveniles. Methods: This study was based on a completely randomized design with 4 treatments and 3 replicates on beluga juveniles with average weight of (mean ±SE) 31.8±2.81g. Beluga Juveniles were divided randomly into 12 fiber glassy tanks with density of 30 fish per tank and were fed with diet contain dietary probiotic with density of 2×106 (Cells/g) for the first treatment, 4×106 (Cells/g) for the second treatment, 6×106 (Cells/g) for the third treatment and basal diet without probiotic for the control group for 8 weeks. Results: Diet supplementing with concentration of 6×106 (Cells/g), significantly improved serum biochemical parameters (p<0.05), however hematological parameters were affected by supplemented diet with probiotics that showed no significant difference in comparison with the control group (p>0.05). Also results indicate that growth factors were improved in experimental treatments in comparison with the control group. Conclusions: The results showed that the use of combination of these species with studied concentrations can improve the performance of some biochemical parameters such metabolites factors, immune, enzymes and serum electrolytes of belugajuveniles. It is recommended that the concentration of A. niger and S. cerevisiae, used for third treatment be used as an immune stimulator for beluga juveniles. | ||
کلیدواژهها [English] | ||
Aspergillus niger, Blood serum, haematology, Huso huso, Saccharomyces cerevisiae | ||
مراجع | ||
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