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Effects of camphor on histomorphometric and histochemical parameters of testicular tissue in mice | ||
| Iranian Journal of Veterinary Medicine | ||
| مقاله 9، دوره 10، شماره 3، آذر 2016، صفحه 225-235 اصل مقاله (3.26 M) | ||
| نوع مقاله: Physiology | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2016.58685 | ||
| نویسندگان | ||
| Hasan Morovvati1؛ masoud adibmoradi* 2؛ Ali Kalantari Hesari1؛ Ramin Mazaheri Nezhad Fard3؛ Hamid Reza Moradi1 | ||
| 1Division of Histology, Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Department of basic sciences ,faculty of veterinary medicin, university of tehran-Iran | ||
| 3Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Background: In traditional medicine of some Asian countries it is believed that camphor could act as a sexual depressant. However, limited studies have been published on this issue. OBJECTIVES: In the current study, effects of camphor on testes, sperm and serum factors, and roles of vitamin E as antioxidant in treatment of toxicity of camphor for testes were studied. METHODS: Fifty adult male mice (20-25 g) were categorized into five groups. Control group, two control sham groups received olive oil and combined vitamin E and olive oil respectively, and two treatment groups received camphor and combined camphor and vitamin E, respectively. Camphor with doses of 30 mg/kg/day and vitamin E with doses of 100 mg/kg/day were prepared. All substances were administered using gavage. After 35 days, blood was collected from the animal heart for serology and testosterone assessment. Sperms were collected and tissue samples were removed and fixed in Bouin and liquid nitrogen. Paraffin embedded and freezing sections were stained with H&E and specific stain and studied. RESULTS: Results showed a significant decrease in sperm count, average proportions of live and mature sperms and major testicular morphometric parameters (p>0.05). Although histochemical changes were seen, no changes were observed in serum testosterone in groups that received camphor. Vitamin E moderated toxicity of camphor in immature sperms, diameter of lumen and TDI index. CONCLUSIONS: It can be concluded that camphor includes adverse effects on parameters of testes and sperm quality. Furthermore, vitamin E, as an antioxidant, can moderate toxicity of camphor. | ||
| کلیدواژه‌ها | ||
| camphor؛ Histochemistry؛ mice؛ testis؛ vitamin E | ||
| عنوان مقاله [English] | ||
| مطالعه هیستومورفومتری و هیستوشیمی تغییرات بیضه موش متعاقب تجویز کافور و بررسی نقش محافظتی ویتامین E | ||
| نویسندگان [English] | ||
| حسن مروتی1؛ مسعود ادیب مرادی2؛ علی کلانتری حصاری1؛ رامین مظاهری نژاد فرد3؛ حمید رضا مرادی1 | ||
| 1بخش بافت شناسی، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران | ||
| 2گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، ایران | ||
| 3بخش میکروبیولوژی، گروه پاتوبیولوژی، دانشکده بهداشت، دانشگاه علوم پزشکی تهران، تهران، ایران | ||
| چکیده [English] | ||
| زمینه مطالعه: در طب سنتی برخی از کشورهای آسیایی این تصور وجود دارد که کافور می‌تواند در کاهش میل جنسی افراد نقش داشته باشد. با این حال مطالعات در این زمینه نیز بسیار محدود می‌باشد. هدف: از این رو در تحقیق حاضر علاوه بر بررسی دقیق‌تر اثرات کافور بر پارامترهای بیضه، اسپرم و فاکتور‌های سرمی، نقش ویتامین E به عنوان یک آنتی‌اکسیدانت در درمان عوارض ناشی از کافور بررسی شد. روش کار: در این تحقیق از 50 قطعه موش سوری نر بالغ (balb/c) 20 تا 25 گرمی در 5 گروه استفاده شد. گروه اول کنترل، دریافت کننده سرم فیزیولوژی (CO)، دو گروه کنترل شم، به ترتیب دریافت کننده روغن زیتون تنها (OL) و گروه دریافت کننده ترکیبی از ویتامین E و روغن زیتون (OL+E)، و نهایتاً دو گروه تجربی دریافت کننده کافور تنها (CA) و گروه دریافت کننده کافور به همراه ویتامین Eر(CA+E). کافور با دوز mg/kg 30 و ویتامین E نیز با دوز mg/kg100 تهیه گردید. تمام مواد به صورت خوراکی (گاواژ) و دوره 35 روزه تجویز شدند. پس از اتمام دوره درمان از طریق قلب خونگیری انجام و نمونه‌های سرمی جهت آزمایشات سرمی و تعیین سطح تستوسترون، نمونه‌های اسپرم برای بررسی اسپرم و نمونه‌های بافتی نیز پس از جدا سازی در داخل فرمالین و ازت مایع فیکس شده و پس از انجام برش‌های پارافینی و انجمادی در نهایت با رنگ آمیزی‌های معمولی و اختصاصی مورد بررسی قرار گرفتند. نتایج: نتایج نشان دهنده کاهش معنی‌دار تعداد اسپرم، درصد اسپرم‌های زنده و بالغ، کاهش اکثر پارامترهای مورفومتریک بیضه، تغییرات هیستوشیمی و عدم تغییر سطح هورمون تستوسترون سرمی در گروه دریافت کننده کافور بود در حالی که ویتامین E به عنوان یک آنتی اکسیدانت بصورت خفیفی توانسته بود اثرات کافور را کاهش دهد (05/0>p). نتیجه گیری نهایی: می‌توان نتیجه گرفت که کافور بر پارامترهای کیفیت اسپرم و بیضه در موش سوری تأثیر داشته و ویتامین E به عنوان یک آنتی اکسیدانت، قادر به تعدیل عوارض ناشی از دریافت کافور در رابطه با پارامترهای مذکور می‌باشد. | ||
| کلیدواژه‌ها [English] | ||
| بیضه, هیستوشیمی, کافور, ویتامین E, موش سوری | ||
| مراجع | ||
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