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بررسی سمیت حاد و تحتکشنده علفکش آترازین بر شاخصهای خونی ماهی شیربت (Tor grypus) | ||
مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
مقاله 7، دوره 71، شماره 3، مهر 1395، صفحه 295-301 اصل مقاله (841.8 K) | ||
شناسه دیجیتال (DOI): 10.22059/jvr.2016.58727 | ||
نویسندگان | ||
علی خبازیان زاده* 1؛ علی دادالهی سهراب2؛ مجتبی علیشاهی3؛ سید حسین خزاعی2؛ حسین محمدعسگری2 | ||
1دانش آموخته محیطزیست دریا دانشگاه علوم و فنون دریایی خرمشهر، خوزستان- ایران | ||
2گروه محیط زیست، دانشکده منابع طبیعی دریا دانشگاه علوم و فنون دریایی خرمشهر، خوزستان- ایران | ||
3گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز- ایران | ||
چکیده | ||
زمینه مطالعه: علفکش آترازین یکی از مهمترین آلایندههای اکوسیستمهای آبی است که متاسفانه به فور در مزراع نیشکر استان خوزستان مورد استفاده قرار گرفته و موجب آلودگی منابع آبی میگردد. هدف: محاسبه سمیت حاد آترازین در ماهی شیربت (Tor grypus) و سپس اثر مسمومیت مزمن این سم بر برخی شاخصهای خونی میباشد. روش کار: LC50 96 ساعته آترازین در ماهی شیربت با وزن g12/1 ±10 با استفاده از نرم افزار Probit تعیین شد. سپس180 قطعه ماهی شیربت با وزن g15±102 در 4 گروه (5، 10 و 20% LC50 96 و گروه شاهد) در سه تکرار تقسیم گردیدند. 7، 14 و 21 روز پس از شروع آزمایش خونگیری انجام شده و شاخصهای خونشناسی شامل: هموگلوبین، هماتوکریت، تعداد گلبولهای قرمز و سفید خونی و اندیسهای گلبولی، در مراحل مختلف مقایسه گردید. نتایج: LC50 96 ساعته آترازین 1- mg l 65 محاسبه شد و تقریباً تمام فاکتورهای خونی تحت تأثیر مسمومیت با این سم قرار گرفت (05/0>p). در مورد اندیسهای گلبولی، تفاوت معنیداری بین تیمارها مشاهده نشد (05/0<p). نتیجهگیرینهایی: در مجموع با توجه به نتایج، آترازین تأثیر عمیقی بر روی فاکتورهای خونی ماهی شیربت دارد، و قرار گرفتن طولانی مدت در معرض غلظت بالای این سم منجر به ایجاد تغییرات فیزیولوژیک در ماهی شیربت میگردد. | ||
کلیدواژهها | ||
آترازین؛ شاخصهای خونی؛ سمیت تحتکشنده؛ ماهی شیربت | ||
عنوان مقاله [English] | ||
Evaluation of acute and sub-lethal toxicity of herbicide, Atrazine, on hematological parameters of Tor grypus | ||
نویسندگان [English] | ||
Ali Khabazian Zadeh1؛ Ali Dadolahi Sohrab2؛ Mojtaba Alishahi3؛ Seyed Hosein Khazaei2؛ Hosein Mohammad Asgari2 | ||
1Graduated from the Marine Environment Khorramshahr University of Marine Science and Technology, Khozestan- Iran | ||
2Department of Environmental Sciences, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khozestan- Iran | ||
3Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
چکیده [English] | ||
BACKGROUND: Atrazine is one of the most important and effective pollutants in aquatic ecosystems. The widest sugar cane farms of the Middle East are located in Khouzestan province, Iran. Large amounts of Atrezine are being used in farming on these farms. Objectives: The aims of this study were to investigate acute toxicity (LC50 96 h) of atrazine on Barbus grypus and the effects of chronic toxicity with sub-lethal concentration of atrazine on hematological parameters as well. Methods: 180 B grypus were divided into 4 equal groups (5, 10 and 20% of LC50 96 h and control). Blood samples were taken on days 0, 7, 14 and 21 of experiment. Hematological parameters including hemoglobin level, hematocrit, and red blood cells (RBC), white blood cells (WBC), and globular indices were measured and compared among the groups. Results: LC50 96 h was measured 65 mg l-1. Hematological parameters were significantly affected by chronic toxicity of atrazine. Hemoglobin, hematocrit and RBC were decreased at all sampling periods in fish exposed to different levels of atrazine (p<0.05). There were no significant changes in globular indice values among the groups in sampling periods (p>0.05). For all samples, WBC value was decreased significantly on day 21 (p<0.05). Conclusions: Based on the results, it can be concluded that chronic atrazine toxicity adversely affects hematological parameters of B. grypus. | ||
کلیدواژهها [English] | ||
atrazine, hematological parameters, sub-lethal toxicity, Barbus grypus | ||
مراجع | ||
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