پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 161 |
| تعداد شماره‌ها | 6,532 |
| تعداد مقالات | 70,500 |
| تعداد مشاهده مقاله | 124,088,866 |
| تعداد دریافت فایل اصل مقاله | 97,192,024 |
تأثیر باکتریهای بازدارنده سیستم حدنصاب احساس بر بیماریزایی Pectobacterium carotovorum عامل پوسیدگی نرم سیبزمینی | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 3، دوره 5، شماره 2، آذر 1395، صفحه 151-162 اصل مقاله (704.12 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2016.60150 | ||
| نویسندگان | ||
| عادله سبحانی پور1؛ کیوان بهبودی* 2؛ اسماعیل محمودی3؛ محسن فرزانه4 | ||
| 1دانشجوی دکتری،گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| 2دانشیار، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| 3استادیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد اصفهان و قطب علمی ترانسژنز، دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان)، اصفهان | ||
| 4استادیار، پژوهشکده گیاهان و مواد دارویی، دانشگاه شهید بهشتی، اوین | ||
| چکیده | ||
| باکتری Pectobacterium carotovorum یکی از مهمترین باکتریهای بیماریزای سیبزمینی در دنیا است. بیان تعدادی از ژنهایی که در تولید فاکتورهای بیماریزایی در این باکتری دخیل هستند با سیستم حدنصاب احساس تنظیم میشود. در P. carotovorum مولکولهای سیگنال دخیل در این سیستم از نوع AHLs میباشند. در این تحقیق 1280 جدایه باکتری از ریزوسفر گیاهان مختلف میزبان این بیمارگر از مناطق مختلف ایران جدا شد. برای غربال آنها از جهت توان غیرفعالسازی AHLs از Chromobacterium violaceum CV026 و Escherchia coli pSB401 به‌عنوان گزارشگر استفاده شد. 69 جدایه به میزان بسیار زیاد قادر به تجزیه AHL بودند. اندازه‌گیری میزان تجزیه AHL با روش HPLC نشان داد که در برخی جدایهها هیچ میزانی از C6-HSL ردیابی نشد و به‌طور کامل توسط آنتاگونیستها تجزیه شده بود. توانایی این جدایههای انتخابی از نظر کاهش لهیدگی غده سیبزمینی بررسی شد. 40 جدایه توانستند بیش از 50 درصد کاهش در لهیدگی غده ایجاد کنند. توانایی 16 جدایه برتر آنها برای کاهش پوسیدگی ساقه نیز آزمایش شد که جدایههای Bacillus toyoensis 2L، Escherchia coli 1M و Acinetobacter calcoaceticus 32P بیش از 70 درصد پوسیدگی ساقه را کاهش دادند. استرین‌ها با توالی‌یابی ناحیه 16srDNA شناسایی شدند. نتایج این تحقیق نشان داد که مداخله در سیستم حدنصاب احساس باکتری P.carotovorum می‌تواند به‌عنوان یک هدف خوب برای توسعه روش کنترل بیولوژیک این بیماری مطرح باشد. | ||
| کلیدواژه‌ها | ||
| CV026؛ pSB401؛ Quorum Sensing؛ کنترل بیولوژیک | ||
| عنوان مقاله [English] | ||
| The Effect of Quorum Quenching Bacteria on Pathogenicity of Pectobacterium carotovorum Causal Agent of Potato Soft Rot | ||
| نویسندگان [English] | ||
| Adeleh Sobhanipour1؛ Keivan Behboudi2؛ Esmaeil Mahmoudi3؛ Mohsen Farzaneh4 | ||
| 1Ph. D. Student, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Associate Professor, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Assistant Professor, Department of Plant Protection, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan and Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran | ||
| 4Assistant Professor, Medicinal Plants Research Institute, Department of Agriculture, Shahid Beheshti University, Iran | ||
| چکیده [English] | ||
| Pectobacterium carotovorum is one of the most important bacterial pathogens of potato in the world.The expression of numerous genes including those involved in the production of virulence determinants in this bacterium are regulated by quorum sensing (QS). The signal molecules involved in QS in P. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). Recently, several soil bacteria were found to degrade AHLs, thereby interfering with the QS system. In this research, 1280 bacterial strains were isolated from potato rhizosphere in Iran. For the screening of isolates for AHL-inactivating, Chromobacterium violaceum CV026 and Escherchia coli pSB401 was used as biosensors. The 69 isolates have highly AHL-degrading potential. The AHL degradation measurement by HPLC showed that residue of C6-HSL was not detected in some isolates that means completely degradation of it by antagonists. The screened isolates tested for protect potato tubers against soft rot. 40 strains reduced tuber soft rot more than 50 percent. 16 of the best isolates were used for inhibition of stem tissue maceration. Bacillus toyoensis 2L, Escherchia coli 1M and Acinetobacter calcoaceticus 32P reduced stem maceration more than 70 percent. These strains were identified by 16s rDNA sequencing. These results showed that interference with P. carotovorum quorum sensing system can be considered as an attractive target for development of biological control method of this disease. | ||
| کلیدواژه‌ها [English] | ||
| biological control, Quorum Sensing, CV026, pSB401 | ||
| مراجع | ||
|
Amaral L, Molnar J (2012) Inhibitors of efflux pumps of gram-negative bacteria inhibit quorum sensing. Open Journal of Pharmacology 12-20.
Barnard AM, Salmond GP (2007) Quorum sensing in Erwinia species. Analytical and Bioanalytical Chemistry 387: 415-423.
Barras F, van Gijsegem F, Chatterjee AK (1994) Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annual Review of Phytopathology32: 201-234.
Chin-A-Woneng TFC, van den Broek D, de Voer G, van der Drift KM, Tuinman S, Thomas-Oates JE, Lugtenberg BJJ, Blomberg GV (2001) Phenazin-1-carboxamide production in the biocontrol strain Pseudomonas chlororaphis PCL1391 is regulated by multiple factors secreted into the growth medium. Molecular Plant-Microbe Interactions 14: 969-979.
Czajkowski R, Jafra S (2009) Quenching of acyl homoserine lactones-dependent quorum sensing by enzymatic disruption of signal molecules. Acta Biochimica Polonica 56: 1-16.
Czerwicka M, Marszewska K, Bychowska A, Dziadziuszko H, Brzozowski K, Łojkowska E, Stepnowski P, Kaczynski Z (2011) Chemical structure of the O-polysaccharide isolated from Pectobacterium atrosepticum SCRI 1039. Carbohydrate Research 346: 2978–2981.
Dong YH, Xu JL, Li XZ, Zhang LH ( 2000) AiiA, an enzyme that inactivates the acylhomoserine lactone quorum- sensing signal and attenuates the virulence of Erwinia carotovora. Proceeding of the NationalAcademy of Science USA97: 3526-3531.
Edwards U, Rogall T, Blocker H, Bottger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Research 17(19): 7843-7853.
Galloway WRJD, Hodgkinson TJ, Bowden S, Welch M, Spring DR (2012) Applications of small molecule .activators and inhibitors of quorum sensing in Gram-negative bacteria. Trends in Microbiology 20(9): 449-458.
Hong KW (2012) Quorum quenching revisited – from signal decays to signalling confusion. Sensors 12: 4661-4696.
Huang W, Lin Y, Yi S, Liu P, Shen J (2012) QsdH, a Novel AHL Lactonase in the RND-Type Inner Membrane of Marine Pseudoalteromonas byunsanensis Strain 1A01261. PLoS ONE 7(10): 46587. doi:10.1371/journal.pone.0046587.
Hugouvieux-Cotte-Pattat N, Condemine G, Nasser W, Reverchon S (1996) Regulation of pectinolysis in Erwinia chrysanthemi. Annual Review of Microbiology50: 213-257.
Jafra S, Przysowa J, Czajkowski R, Michta A, Garbeva P, van der Wolf JM ( 2006) Detection and characterization of N-acyl homoserine lactone-degrading bacteria from the potato rhizosphere. Canadian Journal of Microbiology52: 1006-1015.
Kalia A, Gosal S K (2011) Effect of pesticide application on soil microorganisms. Archives of Agronomy and Soli Science 57(6): 569-596.
Kalia VC (2013) Quorum sensing inhibitors: An overview. Biotechnology Advances 31: 224-245.
Kröner A, Marnet N, Andrivon D, Val F (2012) Nicotiflorin, rutin and chlorogenic acid: phenylpropanoids involved differently in quantitative resistance of potato tubers to biotrophic and necrotrophic pathogens. Plant Physiology and Biochemistry 57: 23-31.
Krzyanowska D M, Potrykus M, Golanowska M, Polonis K, Gwizdek-Wisniewska A, Lojkowska E, Jafra S (2012) Rhizosphere Bacteria as Potential Biocontrol Agents Against Soft Rot Caused by Various Pectobacterium and Dickeya spp. Strains. Journal of Plant Pathology 94 (2): 367-378.
Lin YH, Xu JL, Hu J, Wang LH, Ong SL, Leadbetter JR, Zhang LH ( 2003) Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes. Molecular Microbiology 47: 849-860.
Liu H, Coulthurst SJ, Pritchard L, Hedley PE, Ravensdale M, Humphris S, Burr T, Takle G, Brurberg MB, Birch PRJ, Salmond GPC, Toth IK (2008) Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. PLo- SPathogen4: e1000093.
Lo C (2010) Effect of pesticides on soil microbial community. Journal of Environmental ScienceandHealth 45:348-359.
Mahmoudi E, Ahamadi A, Seyed-Tabatabaei BE, Ghobadi A, Akhavan Hassanzadeh N, Venturi V (2011) An Novel AHL-Degrading Rhizobcterium quenches the virulence Of Pectobacterium atrosepticum on potato plant. Journal of Plant Pathology 93(3): 587-594.
Molina L, Constantinescu F, Michel L, Reimmann C, Duffy B, Défago G (2003) Degradation of pathogen quorum sensing molecules by soil bacteria: a preventive and curative biological control mechanism. FEMS Microbiology Letters45: 71-81.
Morohoshi T, Nakazawa S, Ebata A, Kato N, Ikeda T (2008) Identification and Characterization of N-Acylhomoserine Lactone-Acylase from the Fish Intestinal Shewanella sp. Strain MIB015. Bioscience of Biotechnology and Biochemistry 72: 1-7.
Morohoshi T, Tominaga Y, Someya N, Ikeda T (2012) Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris. Journal of Bioscience and Bioengineering 113(1): 20-25.
Nasser W, Bouillant M L, Salmond G, Reverchon S (1998) Characterization of the Erwinia chrysanthemi expI-expR locus directing the synthesis of two N-acyl-homoserine lactone signal molecules. Molecular Microbiology 29: 1391-1405.
Park SJ, Park SY, Ryu CM, Park SH, Lee JK (2008) The Role of AiiA, a quorum-quenching enzyme from Bacillus thuringiensis, on the Rhizosphere Competence. Journal of Microbiology and Biotechnology 18(9): 1518-1521.
Pirhonen M, Flego D, Heikinheimo R, Palva ET (1993) A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. European Molecular BiologyOrganization Journal 12(6): 2467-2476.
Qian GL, Fan JQ, Chen DF, Kang YJ, Han B, Hu SH, Liu FQ (2009) Reducing Pectobacterium virulence by expression of an N-acyl homoserine lactonase gene PIpp- aiiA in Lysobacter enzymogenes strain OH11. Biological Control 52: 17-23.
Reimmann C, Ginet N, Michel L, Keel C, Michaux P, Krishnapillai V, Zala M, Heurlier k, Triandafillu K, Harms H, Defago G (2002) Genetically programmed autoinducer destruction reduces virulence gene expression and swarming motility in Pseudomonas aeruginosa PAO1. Microbiology 148: 923-932.
Romero M, Acuña L, Otero A (2012) Patents on quorum quenching: interfering with bacterial communication as a strategy to fight infections. Recent Patents Biotechnology 6: 2-12.
Schaad NW, Jones JB, Chun W (2001) Laboratory guide for identification of palnt pathogenic bacteria. 3ed. The American Phytopathological Society, USA.
Uroz S, Angelo-Picard C, Carlier A, Elasri M, Sicot C, Petit A, Oger P, Faure D, Dessaux Y (2003) Novel bacteria degrading N-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plantpathogenic bacteria. Microbiology149: 1981-1989.
Uroz, S., Dessaux, Y. and Oger, P (2009) Quorum sensing and quorum quenching: the Yin and Yang of bacterial communication. Biochemistry 10: 205-216.
Van Gijsegem F, Wlodarczyk A, Cornu A, Reverchon S, Hugouvieux-Cotte-Pattat N (2008) Analysis of the LacI family regulators of Erwinia chrysanthemi 3937, involvement in the bacterial phytopathogenicity. Molecular Plant-Microbe Interaction21: 1471-1481
Varga GZ, Szabo MA, Hohmann J, Schelz Z, Szegedi E, Amaral L, Molnár J (2011) Effect of phenothiazines and related compounds on quorum sensing regulated functions. Lett Drug Discovery Design 8: 133-7.
Yangui T, Sayadi S, Dhouib A (2013) Sensitivity of Pectobacterium carotovorum to hydroxytyrosol-rich extracts and their effect on the development of soft rot in potato tubers during storage. Crop Protection 53: 52-57.
Zheng HM, Zhong ZT, Lai X, Chen WX, Li SP, Zhu J (2006) A LuxR/LuxItype quorum sensing system in a plant bacterium, Mesorhizobium tianshanense, controls symbiotic nodulation. Journal of Bacteriology 188: 1943-1949.
Zhu CG, Yu ZN, Sun M (2006) Restraining Erwinia virulence by expression of N-acyl homoserine lactonase gene pro3A-aiiA in Bacillus thuringiensis subsp. leesis. Biotechnology and Bioengineering 95: 526-532. | ||
|
آمار تعداد مشاهده مقاله: 1,539 تعداد دریافت فایل اصل مقاله: 1,476 |
||