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بررسی اثر اتیلن خارجی بر ویژ‌گی‌های بیوشیمیایی و فعالیت آنزیمی مرتبط با فرایند پیری در دو اکوتیپ گل‌محمدی | ||
| به زراعی کشاورزی | ||
| مقاله 15، دوره 19، شماره 3، آذر 1396، صفحه 767-783 اصل مقاله (730.98 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jci.2017.60397 | ||
| نویسندگان | ||
| غلامحسن موحد* 1؛ نیما احمدی2؛ احمد معینی3 | ||
| 1کارشناسی‌ارشد، گروه علوم باغبانی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2استادیار، گروه علوم باغبانی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 3دانشیار، گروه اصلاح نباتات، دانشگاه تربیت مدرس، تهران، ایرانن | ||
| چکیده | ||
| به‌منظور ارزیابی واکنش دو اکوتیپ گل‌محمدی (Rosa damascena Mill) به اتیلن خارجی، تحقیقی در قالب فاکتوریل بر پایة طرحی کاملاً تصادفی در آزمایشگاه پس از برداشت گروه علوم باغبانی دانشگاه تربیت مدرس در سال 93-1392 انجام گرفت. برای این منظور چهار وضعیت گل یعنی غنچة منفرد و روی شاخه، گل باز منفرد و روی شاخه به‌مدت 24 ساعت تحت تیمار با اتیلن خارجی به غلظت‌های 1، 2، 3 و 4 میکرولیتر قرارگرفت و فعالیت‌های آنزیمی، بیوشیمیایی و خصوصیات موفولوژیکی اکوتیپ‌های کاشان و آذران بررسی شد. کمترین عمر گل در غلظت 3 و 4 میکرولیتر بر لیتر اتیلن و بیشترین ریزش نیز مربوط به اکوتیپ آذران در غلظت 4 میکرولیتر بر لیتر اتیلن پس از چهار روز مشاهده شد. کمترین میزان آنتوسیانین، پرولین و فعالیت آنزیم‌کاتالاز (77/48 در دلتای جذب 240 بر میلی‌گرم پروتئین) در هر دو اکوتیپ مورد مطالعه در غلظت 4 میکرولیتر بر لیتر اتیلن مشاهده شد. بیشترین مقدار مالون‌د‌ی‌آلدئید (MDA) (63/3 میکرومول) در تیمار با اتیلن (4 میکرولیتر بر لیتر) در اکوتیپ آذران مشاهده شد. کمترین میزان فلاونوئید (19/2 میکرومول) مربوط به آذران بود. همچنین، کاهش پروتئین کل نیز در کاشان (32/0 میلی‌گرم در یک گرم وزن تر گلبرگ) نسبت به آذران (20/0 میلی‌گرم در یک گرم وزن تر گلبرگ) کمتر بوده است. بر اساس نتایج به‌دست آمده، اکوتیپ کاشان نسبت به آذران اکوتیپی متحمل‌تر به تنش اتیلن محسوب می‌شود که لازم است به استفاده از آن در برنامه‌های اصلاحی ورد توجه شود. | ||
| کلیدواژه‌ها | ||
| آذران؛ آنتی‌اکسیدان؛ پروتئین کل؛ پرولین؛ کاشان | ||
| عنوان مقاله [English] | ||
| The effects of exogenous ethylene on biochemical characteristics and enzymatic activity associated with aging of two ecotypes of Damask rose | ||
| نویسندگان [English] | ||
| Gholamhasan Movahed1؛ Nima Ahmadi2؛ Ahmad Moieni3 | ||
| چکیده [English] | ||
| In order to evaluate the responses of two ecotypes of Damask rose (Rosa damascena) to exogenous ethylene, an experiment was conducted based on a completely randomized design (CRD) at the laboratory of Postharvest Physiology, Tarbiat Modares University, Tehran, Iran. Damsk rose cut stems containg flower buds and/or open flowers, single buds, or multiple flowers were exposed to exogenous ethylene for 24 h. Plant organs were placed into hearmatically seald glass chambers, where ethylen was injected, to achieve 0, 1, 2, 3, and 4 µl/l concentrations. Biocehmival characteristics, enzyme activities and morphological characteristics including flower life, contents of anthocyanin, flavonoide, and proline were determind in Kashan and Azaran ecotypes of Damask rose. The lowest psotharvest life was observed at 3 and 4 μl concentration of ethylene and the highest abscission was observed in Azaran ecotypes at a concentration of 4 μl/l of ethylene after four days. Anthocyanin and proline content and catalase activity reduced in both ecotypes studied. For both ecotypes, the lowest content of anthocyanin, proline and the lowest catalase activity (48/77 ΔA 240/mg protein) were observed in those treated with ethylene at 4 µl/l. The highest malondialdehyde (3.63 µmol/g petal fresh weight) was found in Azaran ecotype flowers treated with 4 µl/l ethylene concentration. Decrease of total protein in Kashan flowers (0.32 mg/g fresh petal) was much less than that in Azaran flowers (0.2 mg/g fresh petal). Based on these results, Kashan could be recommended as a tolerant ecotype to be used in breeding programs. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidants, azaran, Kashan, proline, total protein | ||
| مراجع | ||
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