پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 161 |
| تعداد شماره‌ها | 6,532 |
| تعداد مقالات | 70,503 |
| تعداد مشاهده مقاله | 124,120,894 |
| تعداد دریافت فایل اصل مقاله | 97,227,780 |
بررسی توان آزادسازی پتاسیم و فسفر برخی جدایههای باکتریایی در شرایط درون‌شیشه‌ای و شناسایی باکتری-های کارآمد | ||
| تحقیقات آب و خاک ایران | ||
| مقاله 14، دوره 48، شماره 2، مرداد 1396، صفحه 385-395 اصل مقاله (366.97 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2017.62647 | ||
| نویسندگان | ||
| شکوفه مرادی؛ محمدرضا ساریخانی* ؛ ناصر علی‌اصغرزاد | ||
| دانشگاه تبریز | ||
| چکیده | ||
| پتاسیم یکی از عناصر پرمصرف ضروری برای رشد و توسعه گیاهان است و در سالهای اخیر تأمین زیستی آن از طریق باکتریهای آزادکننده پتاسیم در کانون توجه تحقیقات قرار گرفته است. در این آزمایش توانایی آزادسازی پتاسیم از کانیهای سیلیکاتی، توسط چندین باکتری جداسازی شده از نمونههای ریزوسفری گیاهان گرامینه، به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی مورد ارزیابی قرار گرفت. فاکتورهای آزمایش شامل جدایههای باکتریایی و دو نوع کانی میکا (بیوتیت و موسکویت) بود. پس از جداسازی باکتریها از ریشههای ضدعفونی شده گیاهان گرامینه با استفاده از محیط کشت NFB، تعداد 8 جدایه شاخص برای آزمایش نهایی مورد استفاده قرار گرفت. توان رهاسازی پتاسیم جدایهها با بهره‌گیری از محیط کشت الکساندروف مایع مورد ارزیابی قرار گرفت. بالاترین میزان پتاسیم آزادشده به میزان 16/11 میلیگرم بر لیتر توسط جدایه Az-8 به دست آمد که 20% نسبت به تیمار شاهد افزایش داشت و پایین‌ترین مقدار 8/2 میلیگرم بر لیتر توسط جدایه Az-15 مشاهده شد. به‌طور متوسط آزادکنندگی پتاسیم از کانی بیوتیت 82% بیشتر از موسکویت مشاهده شد. در بین باکتریهای جداسازی شده جدایههای Az-8، Az-12 و Az-19 توانایی بالایی در آزادسازی پتاسیم نشان دادند. همچنین در این آزمایش بین تغییرات pH و میزان رهاسازی پتاسیم توسط جدایههای باکتریایی همبستگی معنیداری مشاهده نشد. شناسایی مولکولی و بیوشیمیایی این جدایهها و نتایج توالی‌یابی آنها نشان داد که این جدایهها متعلق به جنس سودوموناس می‌باشند. با توجه به نتایج امیدوارکننده آزمایش‌های درون‌شیشه‌ای سویه‌های فوق، بهره‌گیری از آنها در تأمین نیاز پتاسیمی گیاهان در کشت گلخانه و مزرعه توصیه می‌شود. | ||
| کلیدواژه‌ها | ||
| باکتریهای آزادکننده پتاسیم؛ بیوتیت؛ موسکویت؛ الکساندروف؛ سودوموناس | ||
| عنوان مقاله [English] | ||
| Assessment of potassium releasing ability of some bacterial isolates in in-vitro condition and identification of efficient isolates | ||
| نویسندگان [English] | ||
| Shokoofeh Moradi؛ Mohammad Reza Sarikhani؛ Naser Aliasgharzad | ||
| University of Tabriz | ||
| چکیده [English] | ||
| Potassium (K) is an essential macronutrient that plays an important role in the growth and development of plants. Throughout the present study the possibility of K-release from mica minerals was evaluated through the action of several bacteria isolated from rhizosphere samples of grasses. The experiment was conducted as a factorial one, based upon a completely randomized design of three replicates comprised of two factors including 8 isolates of bacteria and two sources of potassium mineral. Following isolation of bacteria from the plant roots through NFB medium, eight selected isolates were ultimately used for the final experiment. Potassium release capability of these isolates was assessed using liquid Aleksandrov culture medium. Acid washed pretreated minerals, as a source of potassium, were added to 30 ml of Aleksandrov medium. Following incubation for one week at 26 °C and shaking at 120 rpm, released K in supernatant was assessed through flame photometer. The highest K release on the average was obtained by the isolate Az-8 (11.16 mg/l) and it was revealed that this bacterium was more efficient in releasing K from biotite than from muscovite, and the lowest rate of K release was obtained by Az-15 (2.8 mg/l). The results also revealed that K released from biotite exceed muscovite when the two types of mica compared. Among the bacterial isolates Az-8, Az-12 and Az-19 showed great potential for K release and their molecular (16S rDNA) and biochemical identification revealed that Az-8, Az-12 and Az-19 belonged to Pseudomonas genus. According to the promising results of in-vitro assays, inoculation and application of these efficient isolates will be recommended in greenhouse and field tests with different crops. | ||
| کلیدواژه‌ها [English] | ||
| Potassium releasing bacteria, Aleksandrov, Pseudomonas, biotite, Muscovite | ||
| مراجع | ||
|
Barker, W.W., S.A. Welch., and J.F. Banfield. (1997). Geomicrobiology of silicate minerals weathering. Reviews in Mineralogy and Geochemistry, 35, 391- 428. Basak, B.B., and D.R Biswas, (2010). Co-inoculation of potassium solubilizing and nitrogen fixing bacteria onsolubilization of waste mica and their effect on growth promotion and nutrient acquisition by a forage crop. Biology and Fertility of Soils, 46(6), 641-648. Diep, C. N., and T.N. Hieu. (2013). Phosphate and potassium solubilizing bacteria from weathered materials of denatured rock mountain, Ha Tien, KiênGiang province Vietnam. American Journal of Life Sciences,1(3), 88-92. Dong, H. (2010). Mineral-microbe interactions: a review. Frontiers of Earth Science in China, 4(2),127–147. Han, H.S., and K.D. Lee. (2005). Phosphate and Potassium Solubilizing Bacteria Effect on Mineral Uptake, Soil Availability and Growth of Eggplant. Research Journal of Agriculture and Biological Sciences, 1(2), 176-180. Hu, X., J. Chen., and J. Guo. (2006). Two phosphate and potassium-solubilizing bacteria isolated from Tianmu Mountain, Zhejiang, China. World journal of Microbiology and Biotechnology, 22(9), 983-990. Huang, P. M., and S. Song. (1988). Dynamics of potassium release from potassium bearing minerals as influenced by oxalic and citric acids. Soil Science Society of America Journal, 52, 383-390. Jones, B.J.J. (2001). Laboratory Guide for Conducting Soil Tests and Plant Analysis. CRC Press, USA. Keshavarz Zarjani, J., Aliasgharzad, N., Oustan, S., M. Emadi., and A. Ahmadi. (2013). Isolation and characterization of potassium solubilizing bacteria in some Iranian soils. Archives of Agronomy and Soil Science, 59(12), 1713-1723. Khan, A.A., Jilani, G., Akhtar, M.S., S.M. SaqlanNaqvi., and M. Rasheed. (2009). Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production, Journal of Agricultural and Biological Science, 1(1), 48-58. Khoshrou, B., M.R. Sarikhani., and N. Aliasgharzad. (2014). Molecular and Biochemical Identification of the Bacterial Isolates Used in Common Biofertilizers in Iran. Journal of Knowledge of soil and water, 25(4/2), 13-26. Krishnaveni, M. S. (2010). Studies on phosphate solubilizing bacteria (PSB) in rhizosphere and non-rhizosphere soils in different varieties of foxtail millet (Setariaitalica). International Journal of Agriculture and Food Science Technology, 1(1), 23-39. Liermann, L.J., Kalinowski, B.E., Brantley, S.L., and J.G. Ferry. (2000). Role of bacterial siderophores in dissolution of hornblende. Geochimica et Cosmochimica Acta, 64(4), 587- 602. Liu, W., Xu, X., Wu, X., Yang, Q., Y. Luo., and P. Christie. (2006). Decomposition of silicate minerals by Bacillus mucilaginosusin liquid culture. Environmental Geochemistry and Health, 28,133–140. Malboobi, M.A., Owlia, P., Behbahani, M., Sarokhani, E., Moradi, S., Yakhchali, B., A. Deljou., and K. MorabbiHeravi. (2009). Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates, World Journal of Microbiology and Biotechnology, 25(8), 1471-1477. Meana, V. S., Maurya, B. R., Verma, J. P., Aeron, A., Kumar, A., K Kim., and V.K. Bajpai. (2015). Potassium solubilizing rhizobacteria (KSR): Isolation, identification, and K-release dynamics from waste mica. Ecological Engineering, 81, 340-347. Moradi, S.H. (2016). Isolation and Identification of Auxin Producing Azospirilla and Study the Effect of Superior Isolates on Growth and Root Development of Corn. Master's Thesis. University of Tabriz. Moradi, S.H., and M.R. Sarikhani. (2016). Comparison of phosphate solubility from rock phosphate and tricalsium phosphate sources by phosphate solubilizing bacteria. The Second Congress of Agricultural Sciences and Natural Resources in the development. Iran. Gorgan. Olsen, S.R., and B.L. Sommers. (1982). Phosphorus. P. 403-430. In: Page et al. (eds) Methods of soil Analysis. Part П. 2ed. ASA, SSSA, Madison .WI .USA. Parmar, P., and S.S. Sindhu. (2013). Potassium solubilization by rhizosphere bacteria: influence of nutritional and environmental conditions. Journal of Microbiology Research, 3(1), 25-31. Perez, E., Sulbaran, M., M.M. Ball., and L.A. Yarzabal. (2007). Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biology and Biochemistry, 39(11), 2905-2914. Rodriguez, H., and R. Fraga. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion, Biotechnology advances, 17(4), 319-339. Rogers, J.R., and P.C. Bennett. (2004). Mineral stimulation of subsurface microorganisms: release of limiting nutrients from silicates. Chemical Geology, 203(1), 91-108. Saghafi, D., H.A. Alikhani., and B. Motesharezadeh. (2014). Evaluation of plant growth promoting characteristics of some Rhizobia isolated from soils of Iran. Journal of Soil Management and Sustainable, 4(2), 131-150. Sarikhani, M.R. (2014). Review of methods for molecular identification of bacteria: Points and considerations. The seventh national conference on agricultural research findings. Iran. Sanandaj. Sarikhani, M.R. (2016). Increasing potassium (K) release from K-containing minerals in the presence of insoluble phosphate by bacteria. Biological Journal of Microorganism, 87-96. Sheng, X.F. (2005). Growth promotion and increased potassium uptake of cotton and rape by a potassium releasing strain of Bacillus edaphicus. Soil Biology and Biochemistry, 37(10), 1918-1922. Sheng, X. F., He, L. Y., and W.Y. Huang. (2003). Conditions of releasing potassium by a silicate dissolving bacteria strain NBT. Agricultureral Sciences in China, 1(6), 662-666. Shu-Xin, T. U., GUO. Zhi-Fen., and S.U.N. Jin-He. (2007). Effect of oxalic acid on potassium release from typical Chinese soils and minerals. Pedosphere, 17(4), 457-466. Sugumaran, P., and B. Janarthanam. (2007). Solubilization of potassium containing minerals by bacteria and their effect on plant growth. World Journal of Agriculture Sciences, 3(3), 350-335. Tarand, Krieg., and Do bereiner. (1979). Genus II. Azospirillum. BERGEY’S MANUAL_ OF Systematic Bacteriology,7-26. Uroz, S., Calvaruso, C., Turpault, M. P., and P. Frey-Klett. (2009). Mineral weathering by bacteria: ecology, actors and mechanisms. Trends in Microbiology, 17(8), 378–387 YiFeng, Z., L. YunXia., and L. HuaZhong. (2009). Separation and purification of the potassium - releasing bacteria. Journal of Hubei University for Nationalities - Natural Science edition, 27(3), 285-288. Zhang, C., and F. Kong. (2014). Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants.Applied Soil Ecology, 82, 18-25.
| ||
|
آمار تعداد مشاهده مقاله: 732 تعداد دریافت فایل اصل مقاله: 922 |
||