پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 158 |
| تعداد شماره‌ها | 6,226 |
| تعداد مقالات | 67,700 |
| تعداد مشاهده مقاله | 115,008,938 |
| تعداد دریافت فایل اصل مقاله | 89,691,043 |
Comparison of MAPK and thioredoxin gene expression in wheat seedlings exposed to silver nitrate and silver nanoparticle | ||
| Progress in Biological Sciences | ||
| مقاله 8، دوره 6، شماره 2، اسفند 2016، صفحه 189-196 اصل مقاله (447.46 K) | ||
| نوع مقاله: Original Research Papers | ||
| شناسه دیجیتال (DOI): 10.22059/PBS.2016.590020 | ||
| نویسندگان | ||
| Javad Karimi؛ Sasan Mohsenzadeh | ||
| چکیده | ||
| The extensive use of heavy metals and nanoparticles (NPs) has led to their release into the environment that might have negative impacts on both organisms and the environment. In this study, the molecular responses of wheat seedlings to silver nitrate and silver nanoparticles (AgNPs) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative Real-time PCR experiment was performed with MAPK (Mitogen-activated protein kinase) and thioredoxin genes using RNA isolated from wheat shoots treated for 0, 2, 6, 12 and 24 h with AgNO3 and AgNPs at 100 mg-1L concentration. Results indicated that stressful conditions led to the antioxidant responses of wheat seedlings that could be reflected as changes in MAPK and thioredoxin gene transcripts. The genes expression patterns were slightly different. The expression of these genes in response to both treatments was high at the beginning of the stress and was decreased with time. Our results showed the effects of AgNO3 treatment were faster than AgNPs. We found that wheat seedlings might develop different strategies to cope with AgNO3 and AgNPs toxicity with change in the expression of MAPK and thioredoxin heavy metal-related genes. | ||
| کلیدواژه‌ها | ||
| Real time PCR؛ Protein kinase؛ Reactive Oxygen Species (ROS)؛ Heavy metals | ||
|
آمار تعداد مشاهده مقاله: 589 تعداد دریافت فایل اصل مقاله: 439 |
||