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ارزیابی توان استرپتومیستهای ریزوسفر گوجهفرنگی در کنترل قارچ بیمارگر Fusarium oxysporum f.sp. radicis-lycopersici | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 5، دوره 6، شماره 2، مهر 1396، صفحه 173-185 اصل مقاله (1.05 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2017.232296.193 | ||
| نویسندگان | ||
| محمد جواد زاهد1؛ کیوان بهبودی* 2 | ||
| 1دانشجوی کارشناسی ارشد، گروه بیماری‌شناسی گیاهی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 2دانشیار، گروه بیماری‌شناسی گیاهی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| چکیده | ||
| پوسیدگی ریشه و طوقه گوجهفرنگی با عامل Fusarium oxysporum f.sp. radicis-lycopersici (FORL) یکی از بیماری‌های مهم خاکزاد گوجهفرنگی بهحساب میآید. در این پژوهش 740 جدایه اکتینوباکتری از مزارع گوجهفرنگی مناطق مختلف استانهای فارس و البرز جمعآوری گردید. آزمون‌های آنزیمی انجام گرفته حاکی از تولید آنزیم پروتئاز و توانایی تولید سیدروفور در تمامی جدایه‌ها بود. محیط کشت فیلتر شده جدایه UTS49 بهعنوان جدایه برتر با جمعیت 108 × 3 به میزان 94 درصد از جوانه‌زنی اسپور قارچ بیمارگر جلوگیری کرد. بیشترین میزان تشکیل بیوفیلم باکتریایی مربوط به جدایههای UTS385، UTS10 و UTS49 بود. در بررسی‌های گلخانه‌ایجدایه UTS49 موجب کاهش علائم بیماری به میزان 75 درصد شد. بررسی جمعیت جدایه UTS49 در ریزوسفر خیار و گوجهفرنگی، بیانگر توان این جدایهدر کلنیزاسیون قوی و پایدار است. بر اساس آزمون‌های فوق جدایه UTS49بهعنوان بهترین جدایه در کنترل بیماری پوسیدگی ریشه و طوقه گوجهفرنگی مشخص شد. با توجه به آزمون‌های بیوشیمیایی و توالی rDNA 16s، این جدایه Streptomyces carpaticusشناسایی و معرفی گردید. | ||
| کلیدواژه‌ها | ||
| پوسیدگی ریشه و طوقه؛ سیدروفور؛ بیوفیلم؛ خاکزاد | ||
| عنوان مقاله [English] | ||
| Assessment of Streptomyces isolates of tomato rhizosphere for biocontrol of Fusarium oxysporum f. sp. radicis-lycopersici | ||
| نویسندگان [English] | ||
| Mohamad Javad Zahed1؛ Keivan Behbodi2 | ||
| 1M. Sc. Student, Department of Plant Pathology, University College of Agricultur & Natrual Resources, University of Tehran, Karaj, Iran | ||
| 2Associate Professor, Department of Plant Pathology, University College of Agricultur & Natrual Resources, University of Tehran, Karaj, Iran | ||
| چکیده [English] | ||
| Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is a causal agent of tomato crown and root rot, a soil-borne disease of worldwide importance in commercial tomatoes. Actinobacteria, in general, and Streptomyces, in particular, are recognized as plant disease controllers and plant growth promoters due to their wide production of the secondary metabolite. In this study, 740 Actinobacteria isolates were gathered from different tomato farms throughout Fars and Alborz provinces. Enzymes production test, such as protein activity, production of siderophore, demonstrated a positive result for all isolates. The results also showed that the UTS49 cell-free culture in 3 × 10 8 concentration was able to prevent the fungal spore germination with the rate 94%. The results indicated that best biofilm was mostly formed by UTS385, UTS10 and UTS49 isolates. In greenhouse conditions, the results showed that UTS49 caused the reduction of the disease up to 25%, which was the most reduction rate of disease symptoms among the studied isolates. S. carpaticus UTS49 demonstrated a strong and stable impact on colonization of cucumber and tomato rhizosphere. According to the biochemical and 16S rDNA, S. carpaticus UTS49 was identified and introduced as the best one against to the pathogen. | ||
| کلیدواژه‌ها [English] | ||
| Crown and root rot, biofilm, Siderophore, Soil-borne | ||
| مراجع | ||
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