Sharifani, Mohammad Mehdi, Kimura, Tetsuya, Yamamoto, Toshiya, Nishtani, Chikako. (1396). Genetic Diversity of Pear (Pyrus spp) Germplasm Assessed by Simple Sequence Repeat (SSR) and Morphological Traits. , 4(2), 145-155. doi: 10.22059/ijhst.2018.245221.210
Mohammad Mehdi Sharifani; Tetsuya Kimura; Toshiya Yamamoto; Chikako Nishtani. "Genetic Diversity of Pear (Pyrus spp) Germplasm Assessed by Simple Sequence Repeat (SSR) and Morphological Traits". , 4, 2, 1396, 145-155. doi: 10.22059/ijhst.2018.245221.210
Sharifani, Mohammad Mehdi, Kimura, Tetsuya, Yamamoto, Toshiya, Nishtani, Chikako. (1396). 'Genetic Diversity of Pear (Pyrus spp) Germplasm Assessed by Simple Sequence Repeat (SSR) and Morphological Traits', , 4(2), pp. 145-155. doi: 10.22059/ijhst.2018.245221.210
Sharifani, Mohammad Mehdi, Kimura, Tetsuya, Yamamoto, Toshiya, Nishtani, Chikako. Genetic Diversity of Pear (Pyrus spp) Germplasm Assessed by Simple Sequence Repeat (SSR) and Morphological Traits. , 1396; 4(2): 145-155. doi: 10.22059/ijhst.2018.245221.210

Genetic Diversity of Pear (Pyrus spp) Germplasm Assessed by Simple Sequence Repeat (SSR) and Morphological Traits

International Journal of Horticultural Science and Technology
مقاله 1، دوره 4، شماره 2، اسفند 2017، صفحه 145-155    اصل مقاله (774.99 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2018.245221.210
نویسندگان
Mohammad Mehdi Sharifani* 1؛ Tetsuya Kimura2؛ Toshiya Yamamoto3؛ Chikako Nishtani3
1Horticulture Department Gorgan University of Agricultural Sciences
2National Center for Seeds and Seedlings, Tsukuba, Ibaraki 305-0852, Japan
3National Institute of Fruit Tree Science, Tsukuba, Ibaraki 305-8605, Japan
چکیده
This study was conducted to identify and recognize relations and diversity among accessions of the Iranianpears (Pyrus spp). A total of 34 pear accessions, derived presumably from at least six species, were subjected to simple sequence repeat (SSR) analysis. The Japanese and the Chinese pear samples were; “Housui” and “Yali” cultivars, example of Pyrus pyrifolia and P. bretschneideri, respectively. Some European pears and Iranian germplasm were analyzed. Seven SSR markers (KU10, BGA35, BGT23b, NH011b, NH013a, NH004a and NH015a) were used for the analysis. One hundred six visible amplified fragments (putative alleles) acquired for 34 pear samples NH011b and NH015a loci exhibited high heterozygosities of 0.82 and 0.79, respectively. BGA35 produced eight putative alleles, while NH013a created 22 putative alleles. The average value of allele per locus was 15. A phenogram was constructed based on the similarity-matrix data using the unweighted pair-group and arithmetic average methods. There was no association between the SSR and morphological phenograms. This work revealed relatedness of Iranian pear samples to the four species of P. communis, P. syriaca, P. salicifolia, and P. glabra.
کلیدواژه‌ها
Genetic diversity؛ Persian pear؛ Pyrus؛ Simple sequence repeats
مراجع
آمار
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