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تاثیر 12 هفته تمرین هوازی و مصرف عصاره چای سبز بر بیان کاسپاز-3 بافت قلبی رت‌های نر مسن | ||
| نشریه علوم زیستی ورزشی | ||
| مقاله 6، دوره 10، شماره 2، شهریور 1397، صفحه 221-235 اصل مقاله (392.25 K) | ||
| نوع مقاله: مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I | ||
| شناسه دیجیتال (DOI): 10.22059/jsb.2018.245693.1220 | ||
| نویسندگان | ||
| میرزا حسین نوروزی کمره1؛ محمدرضا ذوالفقاری* 2؛ فیروز قادری پاکدل3؛ جواد طلوعی آذر4 | ||
| 1-دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2گروه فیزیولوژی ورزشی ، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3دانشیار فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران. | ||
| 4استادیار فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران. | ||
| چکیده | ||
| هدف: بروز نارسایی قلبی در اثر پیری افزایش می‌یابد. آپوپتوز سلول قلبی در پاتوژنز نارسایی قلبی نقش دارد. هدف پژوهش حاضر بررسی تاثیر 12 هفته تمرین هوازی و مصرف عصاره چای سبز بر بیان کاسپاز-3 بافت قلبی رت‌های نر مسن بود. روش شناسی: 32 سر رت نر مسن به طور تصادفی به 4 گروه 8 تایی کنترل، تمرین، مکمل و تمرین+ مکمل تقسیم شدند. گروه‌های تمرینی 12 هفته و 5 روز در هفته فعالیت هوازی با شدت 60 تا 75 درصد اوج اکسیژن مصرفی انجام دادند، در همین مدت گروه‌های دریافت کننده مکمل، مکمل عصاره چای سبز دریافت کردند. برای اندازه‌گیری بیان کاسپاز-3 از روش ایمنوهیستوشیمی استفاده شد. داده‌ها با استفاده از آزمون آماری ANOVA و آزمون تعقیبی توکی آنالیز شدند. یافته‌ها: نتایج نشان داد که بیان کاسپاز-3 به صورت معنی‌داری در گروه‌های تمرین (18 درصد)، مکمل (13 درصد) و تمرین تمرین+ مکمل (43 درصد) نسبت به گروه کنترل کاهش داشت (05/0p≤). همچنین، بیان کاسپاز-3 به صورت معنی‌داری در گروه تمرین + مکمل نسبت به گروه-های تمرین (30 درصد) و مکمل (34 درصد) کاهش داشت (05/0p≤). نتیجه‌گیری: براساس نتایج به دست آمده از مطالعه حاضر می‌توان گفت که ترکیب 12 هفته تمرین هوازی با شدت متوسط با مصرف مکمل عصاره چای سبز نسبت به تمرین هوازی و مصرف مکمل چای سبز به تنهایی می‌تواند بیان کاسپاز-3 در بافت قلب رت‌های نر مسن را کاهش دهد. | ||
| کلیدواژه‌ها | ||
| پیری؛ تمرین هوازی؛ چای سبز؛ کاسپاز-3 | ||
| عنوان مقاله [English] | ||
| Effect of 12 Weeks Aerobic Training and Oral Green tea Extract on Cardiac Caspase-3 Expression in Aged Male Rats | ||
| نویسندگان [English] | ||
| Mirza Hossein Norouzi Kamareh1؛ Mohammad Reza Zolfaghari2؛ Firouz Ghaderi Pakdel3؛ Javad Tolouei azar4 | ||
| 1PhD Student of exercise physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran. | ||
| 2Department of Sport Sciences, Urmia University, Urmia, Iran | ||
| 3Associate Professor of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran | ||
| 4Associate Professor of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran | ||
| چکیده [English] | ||
| Aim: The incidence of heart failure increases with age. Cardiomyocyte apoptosis contributes to the pathogenesis of heart failure. The aim of this study was to evaluate the effect of 12 weeks aerobic training and oral green tea extract on cardiac caspase-3 expression in aged male rats. Methodology: In this study, 32 aged male rats were randomly divided into 4 groups: control, exercise, supplement and exercise + supplement. Exercise groups performed at intensity of 60-75% of vo2peak aerobic exercise for 12 weeks and 5 days per week; meantime, the supplementary groups consumed green tea extract. Immunohistochemistry method was used to measure the expression of caspase-3. Data was analyzed by ANOVA and Tukey's post hoc test. Results: The results show that caspase-3 expression was significantly lower in exercise group (18%), supplement group (13%) and exercise + supplement group (43%), than the control group (p≤0.05). Also, caspase-3 expression was significantly lower in exercise + supplement group than exercise group (30%) and supplement group (34%) (p≤0.05). Conclusion: According to the results of this of this study, combination of 12 weeks of moderate-intensity aerobic training with supplementation of green tea extract can decreased the expression of cardiac caspase-3 than aerobic training and supplementation of green tea alone. | ||
| کلیدواژه‌ها [English] | ||
| Aging, Aerobic training, caspase-3, green tea | ||
| مراجع | ||
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