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Sarvari, Raana, Agbolaghi, Samira, Beygi-Khosrowshahi, Younes, Massoumi, Bakhshali, Bahadori, Ali. (1397). 3D Scaffold Designing based on Conductive/Degradable Tetrapolymeric Nanofibers of PHEMA-co-PNIPAAm-co-PCL/PANI for Bone Tissue Engineering. , 51(2), 101-114. doi: 10.22059/JUFGNSM.2018.02.02
Raana Sarvari; Samira Agbolaghi; Younes Beygi-Khosrowshahi; Bakhshali Massoumi; Ali Bahadori. "3D Scaffold Designing based on Conductive/Degradable Tetrapolymeric Nanofibers of PHEMA-co-PNIPAAm-co-PCL/PANI for Bone Tissue Engineering". , 51, 2, 1397, 101-114. doi: 10.22059/JUFGNSM.2018.02.02
Sarvari, Raana, Agbolaghi, Samira, Beygi-Khosrowshahi, Younes, Massoumi, Bakhshali, Bahadori, Ali. (1397). '3D Scaffold Designing based on Conductive/Degradable Tetrapolymeric Nanofibers of PHEMA-co-PNIPAAm-co-PCL/PANI for Bone Tissue Engineering', , 51(2), pp. 101-114. doi: 10.22059/JUFGNSM.2018.02.02
Sarvari, Raana, Agbolaghi, Samira, Beygi-Khosrowshahi, Younes, Massoumi, Bakhshali, Bahadori, Ali. 3D Scaffold Designing based on Conductive/Degradable Tetrapolymeric Nanofibers of PHEMA-co-PNIPAAm-co-PCL/PANI for Bone Tissue Engineering. , 1397; 51(2): 101-114. doi: 10.22059/JUFGNSM.2018.02.02

3D Scaffold Designing based on Conductive/Degradable Tetrapolymeric Nanofibers of PHEMA-co-PNIPAAm-co-PCL/PANI for Bone Tissue Engineering

Journal of Ultrafine Grained and Nanostructured Materials
مقاله 2، دوره 51، شماره 2، اسفند 2018، صفحه 101-114    اصل مقاله (1.73 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22059/JUFGNSM.2018.02.02
نویسندگان
Raana Sarvari1؛ Samira Agbolaghi* 2؛ Younes Beygi-Khosrowshahi2؛ Bakhshali Massoumi1؛ Ali Bahadori3
1Department of Chemistry, Payame Noor University, Tehran, Iran.
2Chemical Engineering Department, Faculty of Engineering, Azarbaijan Shahid Madani University, Tabriz, Iran.
3University of Applied Science and Technology, Tabriz, Iran.
چکیده
The hydrophilic, conducting, biocompatible and porous scaffolds were designed using poly(2-hydroxy ethyl methacrylate)-co-poly(N-isopropylacrylamide)-co-poly(ε-caprolactone) (P(HEMA-b-NIPAAm-b-CL))/polyaniline (PANI) for the osteoblast applications. To this end, the PHEMA and P(HEMA-b-NIPAAm) were synthesized via reversible addition of fragmentation chain transfer (RAFT) polymerization, and in next step, the ε-caprolactone was polymerized from –OH group of PHEMA segments through the ring opening polymerization (ROP). The electroactivity, mechanical properties, and hydrophilicity of designed scaffolds played an important role in the adhesion, differentiation, and proliferation of MG63 cells. By using the PHEMA and PNIPAAm, the hydrophilicity and biocompatibility, and by employing the PCL, the appropriate mechanical properties were acquired. The addition of PANI in the composition induced the conductivity to scaffolds. The morphology, electrical conductivity, biocompatibility, hydrophilicity and mechanical characteristics of the nanofibers were thoroughly investigated. The scaffolds possessed a porous nanostructure (nanofiber diameter ranged in 60–130 nm) with a large surface area, electrical conductivity of 0.03 S cm–1 and contact angle of 49 ± 5 ͦ , which imitated the natural microenvironment of extra cellular matrix (ECM) to regulate the cell attachment, proliferation and differentiation. In vitro cytocompatibility studies were performed over 168 h and indicated that the nanofibers were non-toxic to MG63 cells and potent to the artificial nanostructured osteoblasting.
کلیدواژه‌ها
Scaffold؛ osteoblast؛ electrospun nanofiber؛ poly(2-hydroxyethylmethaacrylate), poly(N-isopropylacrylamide)؛ polycaprolactone
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