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ارزیابی اثر ضد باکتریایی عصارههای گیاهی علیه باکتری Erwinia amylovora و نقش آنها در اِلقای مقاومت در گلابی | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
مقاله 4، دوره 7، شماره 2، آذر 1397، صفحه 31-47 اصل مقاله (1009.32 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2018.253246.225 | ||
نویسندگان | ||
مهدی اخلاقی؛ سعید طریقی* ؛ پریسا طاهری | ||
گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد | ||
چکیده | ||
در پژوهش حاضر اثرات ضد باکتریایی عصاره اتانولی هفت گیاه دارویی اَفسنطین، بابونه، زیره سبز، بادرنجبویه، آنغوزه، عرعر و اُکالیپتوس علیه باکتری Erwinia amylovora مورد ارزیابی قرار گرفت. آزمون بیماریزایی جدایهها بر روی میوه نارس گلابی رقم اسپادونا انجام شد و بیماریزاترین جدایه جهت ادامه آزمایشها انتخاب گردید. حساسیت جدایه منتخب به دو ترکیب مسی و پنج آنتیبیوتیک سنجیده شد. اثر ضد باکتریایی عصارهها در سه غلظت مختلف با استفاده از روش انتشار دیسک مورد ارزیابی قرار گرفت. جهت بررسی اثر همافزایی با ترکیبات مسی از عصارهی گیاه بادرنجبویه که دارای بیشترین اثر ضد باکتریایی بود استفاده شد. همچنین نقش عصاره گیاه بادرنجبویه در القای مقاومت، بر روی نهال گلابی رقم اسپادونا بررسی شد. نتایج نشان داد عصاره بادرنجبویه با میانگین قطر هاله بازدارنده 66/15 میلیمتر دارای بیشترین اثر ضد باکتریایی بر روی جدایه GA3 بود. عصاره گیاهان بابونه و عرعر در تمامی غلظتهای بکار برده شده فاقد اثر کُشندگی بودند. همچنین نتایج نشان از عدم تاثیر همافزایی عصاره بادرنجبویه و ترکیبات مسی مورد آزمایش داشت. نتایج اثرات القای مقاومت در گیاه توسط عصاره باردنجبویه مثبت ارزیابی شد. بررسی آنزیم آنتیاُکسیدانتی گایاکول پراکسیداز در هفت زمان مورد بررسی بیانگر افزایش سطح این آنزیم در 24 ساعات اولیه نسبت به شاهد و تیمار باکتری بود. نتایج این تحقیق میتواند راهکارهای جدیدی را در تحقیقات آینده به منظور کنترل بیماری آتشک درختان میوه دانه دار، از طریق استفاده از مواد موثره گیاهان دارویی ارایه دهد. | ||
کلیدواژهها | ||
آتشک؛ مهار زیستی؛ گیاه دارویی؛ عصاره گیاهی | ||
عنوان مقاله [English] | ||
Evaluating Antibacterial Effect of Plant Extracts Against Erwinia amylovora and their Role in Resistance Induction in Pear | ||
نویسندگان [English] | ||
Mahdi Akhlaghi؛ Saeed Tarighi؛ Parissa Taheri | ||
Dept. Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad | ||
چکیده [English] | ||
In the present study, antimicrobial effects of the ethanolic herbal extracts of seven medicinal plants including Absinthe, Chamomile, Cumin, Asafoetida, Gum tree, Balm and Tree of heaven against Erwinia amylovora bacterium were investigated. Virulence assay of the bacterial isolates on the immature pear fruit of the Spadona pear cultivar was performed, and the most pathogenic bacterium isolate was selected for further evaluation. Sensitivity of the selected isolate to two copper compounds and five antibiotics was examined. Antibacterial properties were assessed at three concentrations using the disk-diffusion method. To investigate the synergistic effect of the plant extract with copper compounds, the extract of Balm, which have been indicated maximum antibactericidal effect was used. The Balm plant extract was also tested on the seedling of Spadona pear. The ethanolic extract of Balm had the most significant antibacterial effects with the mean inhibition zone diameter of 15.66 mm against the GA3 isolate. The bactericidal effects were not exerted by Chamomile and Heaven tree. Moreover, the results indicated no synergistic effects in the plant extracts of Balm and the tested copper compounds. Our results demonstrated the positive stimulating effects of the plant resistance system by the Balm extract. Evaluation of guaiacol peroxidase at seven different intervals indicated the elevated level of GPX within the first 24 hours compared to the control and bacterial treatment. The results of this research can introduce novel suggestions for future researches in order to control fire blight disease of pome fruit trees via using active components of medicinal plants. | ||
کلیدواژهها [English] | ||
biological control, Fire blight, Herbal plant, Plant extract | ||
مراجع | ||
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