تعداد نشریات | 161 |
تعداد شمارهها | 6,476 |
تعداد مقالات | 70,006 |
تعداد مشاهده مقاله | 122,897,338 |
تعداد دریافت فایل اصل مقاله | 96,105,639 |
اثر Bacillus methylotrophicus در کنترل Fusarium oxysporum f.sp. melonis و بیان برخی ژنهای دفاعی خربزه | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
مقاله 7، دوره 7، شماره 2، آذر 1397، صفحه 83-92 اصل مقاله (1.09 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2019.273278.255 | ||
نویسندگان | ||
جواد آبخو* 1؛ احمد مهربان2 | ||
1، پژوهشکده کشاورزی، دانشگاه زابل، ایران، | ||
2استادیار، گروه کشاورزی، دانشگاه آزاد اسلامی واحد زاهدان، زاهدان، پست الکترونیک Amehraban2004@yahoo.com : | ||
چکیده | ||
این تحقیق با هدف بررسی اثر Bacillus methylotrophicus در کنترل قارچ Fusarium oxysporum f.sp. melonis و بیان برخی ژنهای دفاعی خربزه انجام شد. این آزمایش به دو روش آغشتهسازی خاک باB. methylotrophicus و آغشتهسازی بذر با آن در شرایط گلخانه انجام شد. همچنین بیان ژنهای (GAL2) galactinol synthase 2،β-1,3- glucanas 2 (βGlu2) ، (CAT) catalase و (Chi) chitinase با تکنیک Real-time PCR ارزیابی شد. گیاهان تیمار شده با B. methylotrophicus از نظر شدت بیماری اختلاف معنیداری با شاهد داشتند. شدت بیماری در روش آغشتهسازی خاک با B. methylotrophicus نسبت به روش آغشتهسازی بذر به-طور معنیداری کمتر بود. میزان بیان ژنهایGAL2 ، βGlu2، CAT و Chi بهطور معنیداری توسطB. methylotrophicus افزایش یافت. نتایج این تحقیق نشان داد که B. methylotrophicus در کنترل بیماری پژمردگی ناشی از قارچF. o. f.sp. melonis و افزایش بیان ژنهای دفاعی در گیاه خربزه موثر است. همچنین نتایج ما پیشنهاد میکند که B. methylotrophicus احتمالاً از طریق القا ژنهای دفاعی باعث افزایش مقاومت خربزه در مقابلF. o. f.sp. melonis میشود. | ||
کلیدواژهها | ||
Bacillus methylotrophicus؛ Fusarium oxysporum f.sp. melonis؛ کنترل بیولوژیک؛ chitinase | ||
عنوان مقاله [English] | ||
Effect of Bacillus methylotrophicus on control of Fusarium oxysporum f.sp. melonis and expression of some defense-related genes in melon plant | ||
نویسندگان [English] | ||
javad abkhoo1؛ Ahmad mehraban2 | ||
1Agricultural Research Institute,University of Zabol, 98616, Iran | ||
2Assistant Professor, Department of Agronomy, Islamic Azad University, Zahedan Branch, Zahedan, Iran *Corresponding author: E-mail: Amehraban2004@yahoo.com | ||
چکیده [English] | ||
In this study, we investigated the effect of Bacillus methylotrophicus on control of Fusarium wilt disease of melon caused by F. oxysporum f. sp. melonis and the expression of some defense-related genes in melon. This experiment was carried out using two methods of soil treatment and seed treatment with B. methylotrophicus under greenhouse conditions. Furthermore, the expression of galactinol synthase 2 (GAL2), β-1,3- glucanas 2 (βGlu2), catalase (CAT) and chitinase (Chi) was evaluated by real-time PCR (qRT-PCR) technique. Disease severity was significantly reduced in plants treated with B. methylotrophicus particularly in soil treatment method. The expression of GAL2, βGlu2, CAT and Chi genes was significantly increased by B. methylotrophicus. The results showed the biocontrol activity of B. methylotrophicus against F. o. f. sp. melonis and the subsequent increase in the expression of defense-related genes in melon plant. Furthermore, our results suggest that B. methylotrophicus enhances disease resistance in melon against F. o. f. sp. melonis probably through induction of defense-related genes. | ||
کلیدواژهها [English] | ||
Bacillus methylotrophicus, biological control, chitinase, Fusarium oxysporum f.sp. melonis | ||
مراجع | ||
Abkhoo J (2018) Assessment of resistance in muskmelon genotypes to Fusarium oxysporum f. sp. melonis under greenhouse condition. In: The first international conference on modern researches in applied and engineering sciences, 8 March, Shanghhai, China. 1-5. Abkhoo J, Sabbagh SK (2016) Control of Phytophthora melonis damping-off, induction of defense responses, and gene expression of cucumber treated with commercial extract from Ascophyllum nodosum. Journal of Applied Phycology 28:1333–1342. Abril M, CurryKJ, SmithBJ, WedgeDE (2008) Improved microassays used to test natural product based and conventional fungicides on plant pathogenic fungi. Plant Disease 92(1):106-112. AkramW, Mahboob A, Javed AA (2013) Bacillus thuringiensis strain 199 can induce systemic resistance in tomato against Fusarium wilt. European Journal of Microbiology and Immunology 3(4):275-280. Almoneafy AA, Xie GL, Tian WX, Xu LH, Zhang GQ, Ibrahim M (2012) Characterization and evaluation of Bacillus isolates for their potential plant growth and biocontrol activities against tomato bacterial wilt. African Journal of Biotechnology 11:7193-7201. Ashrafizadeh A, Etebarian HR, Zamanizadeh HR )2005( Evaluation of Trichoderma isolates for biocontrol of Fusarium wilt of melon. Iranian Journal of Plant Pathology 41: 39-57. (In Persian). Banihashemi Z (2010) Reaction of cucumis melo cultivars to races of Fusarium oxysporum f. sp. melonis the cause of melon vascular wilt. Iranian Journal Plant Pathology 46(1):11-22. (In Persian). Boller T, Felix G (2009) A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annual Review Plant Biology 60: 379-406. Bora T, Özaktan H, Göre E, Aslan E (2004) Biological Control of Fusarium oxysporum f. sp. melonis by wettable powder formulations of the two strains of Pseudomonas putida. Journal of Phytopathology 152: 471-475. Brimner T, Boland G (2003) A review of the nontarget effects of fungi used to biologically control plant diseases. Agriculture, Ecosystems & Environment 100: 3-16. Burgess DR, Hepworth G (1996) Biocontrol of Sclerotinia stem rot (Sclerotinia minor) in sunflower by seed treatment with Gliocladium virens. Plant Pathology 45: 583-592. Chandrasekaran M, Belachew ST, Yoon E, Chun S (2017) Expression of β-1,3-glucanase (GLU) and phenylalanine ammonia-lyase (PAL) genes and their enzymes in tomato plants induced after treatment with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria. Journal of General Plant Pathology 83: 7–13. Doxey AC, Yaish MW, Moffatt BA, Griffith Mو Mcconkey BJ )2007( Functional divergence in the Arabidopsis beta-1,3-glucanase gene family inferred by phylogenetic reconstruction of expression states. Molecular Biology and Evolution 24(4): 1045-1055. Ge B, Liu B, Nwet TT, Zhao W, Shi L, Zhang K (2016) Bacillus methylotrophicus strain NKG-1,isolated from changbai mountain, China, has potential applications as a biofertilizer or biocontrol agent. PLoS One 11(11): e0166079. Kim MS, Cho SM, Kang EY, Im YJ, Hwangbo H, Kim YC, Ryu CM, Yang KY, Chung GC, Cho BH (2008) Galactinol is a signaling component of the induced systemic resistance caused by Pseudomonas chlororaphis O6 root colonization. Molecular Plant-Microbe Interactions 21(12): 1643-53. Kong Q, Yuan J, Niu P, Xie J, Jiang W, Huang Y, Biem Zh (2014) Screening suitable reference genes for normalization in reverse transcription quantitative Real-Time PCR analysis in melon. PLoS ONE 9 (1): e87197. Leubner-Metzger G, Meins F Jr (1999) Functions and regulation of plant beta-1,3-glucanases (PR-2), In: Datta SK, Muthukrishnan S (eds.), Pathogenesis-related proteins in plants. CRC Press LLC, USA. pp. 49–76. LiQ, Chen Y, Wang J, Zou F, Jia Y, Shen D, Zhang Q, Jing M, Dou D, Zhang M (2019) A Phytophthora capsici virulence effector associates with NPR1 and suppresses plant immune responses. Phytopathology Research 1(1): 1-11. Li Y, Geng X, Ji P, Pan C, Wei S (2016) Isolation and evaluation of a Bacillus methylotrophicus strain for control of corn stalk rot. Journal Biocontrol Science and Technology 26: 727-731. Liu Y, Yaoa Y, Hua X, Xinga S, Xua L (2015) Cloning and allelic variation of two novel catalase genes (SoCAT-1 and SsCAT-1) in Saccharum officinarum L. and Saccharum spontaneum L. Journal Biotechnology & Biotechnological Equipment 29: 431-440. MossWP, ByrneJM, CampbellHL, JiP, BonasU, JonesJB, WilsonM (2007) Biological control of bacterial spot of tomato using hrp mutants of Xanthomonas campestris pv. vesicatoria. Biological Control 41:199-206. Neto JRMC, Chaves RB, Sardinha DHS, Melo LGL, Rodrigues AAC (2018) Bacterial formulations in induction of resistance and growth promotion of tomato plants. Journal of Agricultural Science 10: 493-503. Nie Q, Gao GL, Fan QJ, Qiao G,Wen XP, Liu T, Peng ZJ, Cai YQ (2015) Isolation and characterization of a catalase gene BHuCAT3 from pitaya (Hylocereus undatus) and its expression under abiotic stress. Gene 563(1):63-71. Nishizawa A, Yabuta Y, Shigeoka S (2008) Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Plant Physiology 147(3): 1251–1263. Norouzi S, Rahnama K, Rabbaninasab H, Taqinasab M (2014) Evaluation of efficacy of Trichoderma and Bacillus isolates in biological control of melon Fusarium wilt. Biocontrol in Plant Protection 2: 43-55. (In Persian). Perchepied L, Pitrat M (2004) Polygenic inheritance of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in melon. Phytopathology 94(12): 1331-1336. Peterbauer T, Lahuta LB, Blochl A, Mucha J, Jones DA, Hedley CL, Gorecki RJ, Richter A (2001) Analysis of raffinose family oligosaccharide pathway in pea seeds with contrasting carbohydrate composition. Plant Physiology 127(4):1764-1772. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (RESTª) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 9: 1–10. Sabbagh SK, Valizadeh Sh (2016) Effect of bio-fertilizers on greenhouse cucumber resistant to damping-off disease caused by Pythium aphanidermatum and increase of yield component. Biological Control of Pests and Plant Diseases 5(1): 111-122. (In Persian). Safarnezhad MR (2004) Study on fungal causes of cucurbit death in Sistan region. In: Proceedings of the 16th Iranian Plant Protection Congress, 28 Aug.–1 Sept., University of Tabriz, Tabriz, Iran. 262. Sahai AS, Manocha MS (1993) Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction. FEMS Microbiology Reviews 11: 317-338. Scandalios JG, Guan L, Polidoros AN (1997) Catalases in plants, gene structure, properties, regulation and expression, In: Scandalios JG (ed.), Oxidative stress and the molecular biology of antioxidant defences cold spring. Harbor Laboratory Pres, USA. pp. 343–406. Shan H, Zhao M Chen D, Cheng J, Li J, Feng Z, Ma Z, An D (2013) Biocontrol of rice blast by the phenaminomethylacetic acid producer of Bacillus methylotrophicus strain BC79. Crop Protection 44: 29-37. SherfAF, MacNabAA (1986) Vegetable diseases and their control. John Wiley and Sons, USA. Xu X, Qin G, Tian S (2008) Effect of microbial biocontrol agents on alleviating oxidative damage of peach fruit subjected to fungal pathogen. International Journal of Food Microbiology 15(1-2):153-8. Szczech M, Shoda M (2006) The effect of mode of application of Bacillus subtilis RB14‐C on its efficacy as a biocontrol agent against Rhizoctonia solani. Journal of Phytopathology 154: 370-377. Zamioudis C. Pieterse CMJ (2012) Modulation of host immunity by beneficial microbes. Molecular Plant-Microbe Interactions 25:39-150. Zhang JX, Xue AG, Tambong JT (2009) Evaluation of seed and soil treatments with novel Bacillus subtilis strains for control of soybean root rot caused by Fusarium oxysporum and F. graminearum. Plant Disease 93(12):1317-1323. Zipfel C (2014) Plant pattern-recognition receptors. Trends in Immunology 35: 345–351. | ||
آمار تعداد مشاهده مقاله: 1,312 تعداد دریافت فایل اصل مقاله: 388 |