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مقایسه کاربرد ستون‌های مختلف برای آنالیز آکریلامید در چیپس سیب زمینی با روش کروماتوگرافی مایع | ||
| مهندسی بیوسیستم ایران | ||
| مقاله 19، دوره 50، شماره 1، فروردین 1398، صفحه 223-239 اصل مقاله (1.29 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijbse.2018.261403.665072 | ||
| نویسندگان | ||
| بابک سامانی پور1؛ مریم جلیلی* 1؛ کرامت الله رضایی2؛ فتانه شکرالهی3 | ||
| 1پژوهشکده صنایع غذایی و کشاورزی، پژوهشگاه استاندارد، کرج، البرز، ایران | ||
| 2استاد تمام گروه علوم و مهندسی صنایع غذائی دانشگاه تهران | ||
| 3سازمان ملی استاندارد، کرجّ البرز | ||
| چکیده | ||
| آکریلامید ترکیبی است سمی که در برخی از مواد غذایی فرآیند شده ایجاد میشود. هدف از این پژوهش، ارائه یک روش آسان و قابل اطمینان برای اندازهگیری آکریلامید در چیپس سیب زمینی بود. بدین منظور، اثر 5 ستون ODS-H-C18 Optimal، Sphere-Image 80-5 ODS 2، Zorbax Hilic Plus، Lichrosorb-100 NH2 و Shodex NH2P-50 4E بر جداسازی و اندازهگیری آکریلامید در چیپس بررسی گردید. بهترین نتایج با کاربرد ستون آمینه نسل جدید Shodex NH2P-50 4E، به دست آمد و پیک آکریلامید با موفقیت و بدون نیاز به انجام مراحل پاکسازی با فاز جامد جداسازی شد. منحنی کالیبراسیون استاندارد در محدوده μg/L 5000-100 کاملا خطی بود (997/0=R2). درصد بازیافت در محدوده 9/95% تا 2/102% ، حد شناسایی و حد تعیین مقدار کمی نیز به ترتیب μg/kg9 و μg/kg 27 به دست آمد. تکرار پذیری این روش مناسب بوده و شاخص انحراف استاندارد نسبی برابر 39/4% بود. | ||
| کلیدواژه‌ها | ||
| "آکریلامید"؛ "روش اندازهگیری"؛ "ستون کروماتوگرافی"؛ "کروماتوگرافی مایع"؛ "طیف سنجی نوری دیودی" | ||
| عنوان مقاله [English] | ||
| Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography | ||
| نویسندگان [English] | ||
| Babak Samanipour1؛ Maryam Jalili1؛ Karamatollah Rezaei2؛ fataneh shokrollahi3 | ||
| 1Food science and agriculture department, Standard research institute, Karaj, Alborz, Iran | ||
| 22Department of Food Science, Engineering, and Technology, University of Tehran, Karaj, Iran | ||
| 3National standard organization,Karaj, Alborz | ||
| چکیده [English] | ||
| Acrylamide is a toxic compound produced in some processed foods. The purpose of this research was to provide an easy and reliable method for measuring acrylamide in potato chips by HPLC-PDA. For this purpose, the effect of 5 columns ODS-H-C18 Optimal, Sphere-Image 80-5 ODS 2, Zorbax Hilic Plus, Lichrosorb-100 NH2 and Shodex NH2P-50 4E were investigated on the separation and measurement of acrylamide in chips. The best results were obtained with the use of the new generation of Shodex NH2P-50 4E amino columns, and acrylamide peak was isolated successfully without the need for solid state purification steps. The standard calibration curve was linear in the range of 100-5000 μg / L (R2 = 0.997). Recycling percentages in the range of 95.9% to 102.2%, the detection limit and the limit of value were 9 and 27 μg / Kg respectively. The repeatability of this method was appropriate and the relative standard deviation was 4.39%. | ||
| کلیدواژه‌ها [English] | ||
| "Acrylamide", "Measurement Method", "Chromatography Column", "Liquid Chromatography", "Photodiode Array Detector" | ||
| مراجع | ||
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