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خسروی, وحید, جوان نیکخواه, محمد, صارمی, حسین, نعیمی, شهرام, زارع, رسول. (1398). معرفی گونه‌های قارچ Fusarium همراه بذر برنج در استان‌های فارس و خوزستان. , 50(1), 61-73. doi: 10.22059/ijpps.2018.235101.1006790
وحید خسروی; محمد جوان نیکخواه; حسین صارمی; شهرام نعیمی; رسول زارع. "معرفی گونه‌های قارچ Fusarium همراه بذر برنج در استان‌های فارس و خوزستان". , 50, 1, 1398, 61-73. doi: 10.22059/ijpps.2018.235101.1006790
خسروی, وحید, جوان نیکخواه, محمد, صارمی, حسین, نعیمی, شهرام, زارع, رسول. (1398). 'معرفی گونه‌های قارچ Fusarium همراه بذر برنج در استان‌های فارس و خوزستان', , 50(1), pp. 61-73. doi: 10.22059/ijpps.2018.235101.1006790
خسروی, وحید, جوان نیکخواه, محمد, صارمی, حسین, نعیمی, شهرام, زارع, رسول. معرفی گونه‌های قارچ Fusarium همراه بذر برنج در استان‌های فارس و خوزستان. , 1398; 50(1): 61-73. doi: 10.22059/ijpps.2018.235101.1006790

معرفی گونه‌های قارچ Fusarium همراه بذر برنج در استان‌های فارس و خوزستان

دانش گیاهپزشکی ایران
مقاله 6، دوره 50، شماره 1، خرداد 1398، صفحه 61-73    اصل مقاله (1.28 M)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22059/ijpps.2018.235101.1006790
نویسندگان
وحید خسروی1، 2؛ محمد جوان نیکخواه* 3؛ حسین صارمی3؛ شهرام نعیمی4؛ رسول زارع5
1دانشجوی دکتری گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
2مربی مؤسسۀ تحقیقات برنج کشور، معاونت مازندران، سازمان تحقیقات، آموزش و ترویج کشاورزی، آمل
3استاد، گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
4استادیار بخش تحقیقات کنترل بیولوژیک، مؤسسۀ تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران
5استاد بخش تحقیقات رستنی‌ها، مؤسسۀ تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران
چکیده
در جریان بازدید از شالیزارهای برنج استان­های فارس و خوزستان در مرحلۀ سفت شدن دانه­، 42 نمونه بذر گرد­آوری شد. برای جداسازی Fusariumاز نمونه­ها، بذرها روی محیط غذایی اختصاصی فوزاریوم (Pepton-PCNB-Agar, PPA) به دو روش ضدعفونی با محلول هیپوکلریت سدیم 1درصد و بدون ضدعفونی کشت و در انکوباتور (اتاقک رشد) با دمای 1±25 درجۀ سلسیوس و نور متناوب (روشنایی و تاریکی) نگهداری شدند. 26 جدایه بر اساس ویژگی‌های ریخت­شناختی روی محیط‌های کشت PDA، CLAو SNAو استفاده از منابع معتبر شناسایی شد. همچنین ارزیابی فیلوژنیک (تبارزایی) 18 و 6 جدایۀ منتخب بر اساس توالی ناحیۀ ژنی TEF1-αو توالی­ نوکلئوتیدی ناحیۀ ITSانجام گرفت. نتایج نشان داد، جدایه­های Fusariumمورد بررسی، متعلق به دو گونۀ مرکب FIESC(Fusarium incarnatum-equiseti species complex) و GFSC(Gibberella fujikuroi species complex)، به ترتیب با فراوانی50 و 46 درصد بودند. F. fujikuroiدر استان فارس و F. semitectumدر استان خوزستان بیشترین فراوانی را داشتند. در این تحقیق دو گونۀ F. fujikuroiو F. andiyaziمتعلق به گونۀ مرکب  GFSCبر اساس توالی ژن TEF1-α، در دو کلاد مجزا تفکیک شدند. توالی­یابی ناحیۀ ژنی TEF1-αبرای تفکیک و شناسایی اعضای گونۀ مرکب  GFSCمفید است اما توالی­ ناحیۀ ITSنتوانست این گونه­ها را شناسایی و از همدیگر تفکیک کند. تک جدایۀ F. merismoidesکه بر اساس ویژگی‌های ریخت­شناختی شناسایی شد، توسط توالی ژن TEF1-αشناسایی نشد اما در درخت فیلوژنیک رسم‌شده بر اساس توالی ناحیۀ  ITSاز جنس Fusariumتفکیک و به جنس Fusicollaو گونۀ F. acetilereaمنتقل شد.
کلیدواژه‌ها
بذرزاد؛ بیمارگر؛ فلور قارچی؛ فیلوژنی
عنوان مقاله [English]
Fusarium species associated with rice seed in Fars and Khuzestan provinces, Iran
نویسندگان [English]
Vahid Khosravi1، 2؛ Mohammad Javan Nikkhah3؛ Hossein Saremi3؛ Shahram Naeimi4؛ Rasoul Zare5
1Ph.D. Candidate, Department of Plant Protection, College of Agriculture and Natural Resources,University of Tehran, Karaj, Iran | Instructor of Rice Research Institute, Mazandaran Branch, Agricultural Research, Education and Extension Organization (AREEO), Amol, Iran
2Ph.D. Candidate, Department of Plant Protection, College of Agriculture and Natural Resources,University of Tehran, Karaj, Iran | Instructor of Rice Research Institute, Mazandaran Branch, Agricultural Research, Education and Extension Organization (AREEO), Amol, Iran
3Professor, Department of Plant Protection, College of Agriculture and Natural Resources,University of Tehran, Karaj, Iran
4Assistant Professor, Department of Biological Control Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
5Professor, Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
چکیده [English]
During survey of rice fields in Fars and Khuzestan provinces at mature grain stage, 42 seed samples were collected. Fusarium isolates were obtained from these samples by planting seeds on selective Fusarium medium (Pepton-PCNB-Agar, PPA) by two methods i.e.; surface sterilization by sodium hypochlorite (NaClO 1%) and without disinfection. Cultivated samples were kept in incubator at 25±1° C and intermittent light (12 hours of light and 12 hours of darkness). Morphological identification of 26 purified Fusarium isolates was performed on the WA, PDA, CLA and SNA. Phylogenetic relationships of 18 isolates on the basis of TEF1-α gene region and ITS1-5.8S-ITS2 region sequences were investigated. The results revealed that Fusarium isolates belonged to two species complexes FIESC (Fusarium incarnatum-equiseti species complex, 50%) and GFSC (Gibberella fujikuroi species complex, 46%). Fusarium fujikuroi species in Fars and F. semitectum species in Khuzestan were most frequent. Two species F. fujikuroi and F. andiyazi belonging to the GFSC species complex were identified in two separate clades in phylogentic tree based on TEF1-α. On the basis of TEF1-α gene, F. merismoides was not identified, but the ITS region sequence was identified as Fusicolla acetilerea and differentiated this species from Fusarium species in the phylogenetic tree.
کلیدواژه‌ها [English]
Mycobiota, seedborne, phylogeny, pathogen
مراجع
  1. Ahmadi, K., Gholizadeh, H., Ebadzadeh, H., Hoseinpour, R., Hatami, F., Fazli, B., Kazemian, A. & Rafiei, M. (2015). Agricultural Statistics Crop Year Book for 2013-2014, Vol. 1. Ministry of Agriculture-Jahad Publishing, Planning and Economic Deputy, Information and Communication Technology Center. 169 pp. (in Farsi)
  2. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Miller, W. & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402.
  3. Alian, S. A. (2005). Study on vegetative compatibility and sexual fertility in populations of Gibberella fujikuroi, the causal agent of rice bakanae disease in Mazandaran province. M. Sc. Thesis, University of Tehran. Iran. 77 pp. (in Farsi)
  4. Amatulli, M. T., Spadaro, D., Gullino, M. L. & Garibaldi, A. (2010). Molecular identification of Fusarium spp. associated with bakanae disease of rice in Italy and assessment of their pathogenicity. Plant Pathology, 59, 839-844.
  5. Aoki, T. & O’Donnell, K. (1999). Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the group 1 population of F. graminearum. Mycologia, 91, 597-609.
  6. Benali, S., Mohamed, B., Eddine, H. J. & Neema, C. (2011). Advances of molecular markers application in plant pathology research. European Journal of Scientific Research, 50(1), 110-123.
  7. Bentley, S., Peggy, K. G. & Dale, J. L. (1995). Genetic variation among a worldwide collection of isolates of Fusarium oxysporum analyzed by RAPD-PCR fingerprinting. Mycological Research, 99, 1375-1384.
  8. Butt, A. R., Yaseen, S. I. & Javaid, A. (2011). Seed-borne mycoflora of stored rice grains and its chemical control. TheJournal of Animal and Plant Sciences, 21(2), 193-196.
  9. Damadzadeh, M. & Hassanpoor, H. (1987). Rice foot rot and its chemical control in Esfahan. Iranian Journal of Plant Pathology, 23, 49- 61.
  10. Damadzadeh, M. (2003). Identification of isolates of Fusarium moniliforme, the causal agent of rice foot rot disease, using RAPD technique. Journal of Nahal and Bazr, 19(2), 227-244. (in Farsi)
  11. Davari, M., Babai-Ahari, A., Arzanlou, M., Zare, R., Van Diepeningen, A. D. & de Hoog, G. S. (2013). Morphological and molecular characterization of three new Fusarium species associated with inflorescence of wild grasses for Iran. Rostaniha,14(2), 124-134. (in Farsi)
  12. Darvishnia, M., Alizadeh, A., Zare, R. & Mohammadi Goltapeh, E. (2006). Three new Fusarium taxa isolated from gramineous plants in Iran. Rostaniha, 7(2), 193-205. (in Farsi)
  13. Diekmann, M. (1993). Epidemiology and geophytopathology of selected seedborne disease. ICARDA, ALEPPO, Syria. Pp 1-15.
  14. Geiser, D. M., Jimenez-Gasco, M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T. J., Zhang, N., Kuldau, G. A. & O’Donnell, K. (2004). FUSARIUM-ID v. 1.0: a DNA sequence database for identifying Fusarium. European Journal of Plant Pathology, 110, 473-479.
  15. Gräfenhan, T., Schroers, H. J., Nirenberg, H. I. & Seifert, K. A. (2011). An overview of the taxonomy, phylogeny, and typification of nectriaceous fungi in Cosmospora, Acremonium, Fusarium, Stilbella, and Volutella. Stud Mycol, 68, 79-113.
  16. Heidarian, Z., Javan-Nikkhah, M., Fotouhifar, K. B. & Ahmadpour, A. (2013). Morphological and phylogenetic investigation on selected Fusarium species belong to Gibberella fujikuroi species complex in Iran. Rostaniha, 14(2), 163-174. (in Farsi)
  17. Hsuan, H. M., Salleh, B. & Zakaria, L. (2011). Molecular identification of Fusarium species in Gibberella fujikuroi species complex from rice, sugarcane and maize from Peninsular Malaysia. International Journal Molecular Science, 12, 6722-6732.
  18. Islam, M. S., Rahman, H., Pervez, Z., Mahmud, M. R. & Alam, A. (2012). Studies on seed borne fungi in rice cultivars grown in non saline Tidal Zones of Patuakhali and their effect on seed germination. Bangladesh Research Publications Journal, 6(3), 286-290.
  19. Jeon, Y. A., Yu, S. H., Lee, Y. Y., Park, H. J., Lee, S., Sung, J. S., Kim, Y. G. & Lee, H. S. (2013). Incidence, molecular characteristics and pathogenicity of Gibberella fujikuroi species complex associated with rice seeds from Asian countries. Mycobiology, 41(4), 225-233.
  20. Karlsson, I., Edel-Hermann, V., Gautheron, N., Durling, M. B., Kolseth, A-K., Steinberg, C., Persson, P. & Friberg, H. (2016). Genus-specific primers for study of Fusarium communities in field samples. Applied and Environmental Microbiology, 82(2), 491-501.
  21. Khosravi, V. (1999). Investigation on important seedborne fungal diseases of dominant rice cultivars in Mazandaran region. M.Sc. thesis. Tehran University, Iran. Pp. 102. (in Farsi)
  22. Khosravi, V., Javan-Nikkhah, M., Saremi, H. & Naeimi, Sh. (2015). Fusarium species associated with rice seeds in Fars and Khuzestan provinces, Iran. In: Proceedings of 2nd Iranian Mycological Congress, Tehran University, Karaj, Iran. 77. (Abst.)
  23. Khosravi, V., Naeimi, S., Rostami, M., Bahrami, M., Zare, L. & Ghalandari, M. (2014).Study of the fungal causal agents of rice panicle discoloration in Mazandaran. In: Proceedings of 21st Iranian Plant Protection Congress, 23-26 Aug., Urmia University, Iran. 100. (Abst.)
  24. Kvas, M., Marasas, W. F. O., Wingfield, B. D., Wingfield, M. J. & Steenkamp, E. T. (2009). Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex. Fungal Diversity, 34, 1-21.
  25. Latiffah, Z. NurulHuda, M. S. & Tengku Ahmad Akram, T. M. A. (2013). Characterization of Fusarium semitectum from Isolates Vegetable Fruits. Sains Malaysiana, 42(5), 629-633.
  26. Leslie, J. F. & Summerell, B. A. (2006). The Fusarium Laboratory Manual. Blackwell Publishing, Oxford, UK. 388. pp.
  27. Leslie, J. F. & Summerell, B. A. (2013). An overview of Fusarium. In Fusarium, Genomics, Molecular and Cellular Biology, Brown D. W. & Proctor, R.H. (eds.), Caister Academic Press, Norfolk, UK, pp. 1–9.
  28. Lombard, L., van der Merwe, N. A., Groenewald, J. Z. & Crous, P. W. (2015). Generic concepts in Nectriaceae. Studies in Mycology, 80, 189-245.
  29. Mew, T. W. & Gonzales, P. (2002). A Handbook of Rice Seedborne Fungi. International Rice Research Institute, Science Publishers Inc. 83 pp.
  30. Nayaka, S. C., Wulff, E. G., Udayashankar, A. C., Nandini, B. P., Niranjana, S. R., Mortensen, C. N. & Prakash, H. S. (2011). Prospects of molecular markers in Fusarium species diversity. Applied Microbiology and Biotechnology, 90, 1625-1639.
  31. Neergaard, P. (1986). Seed Pathology. S. Chand & Company Ltd. New Delhi. 466 pp.
  32. Nelson, P. E., Toussoun, T. A. & Marasas, W. F. O. (1983). Fusarium Species: An Illastrated Manual for Identification. Pennsylvania State University Press. USA. 193 pp.
  33. O’Donnell, K. & Cigelnik, E. (1997). Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution, 7, 103-116.
  34. O’Donnell, K., Cigelnik, E., Weber, N. S. & Trappe, J. M. (1997). Phylogenetic relationships among ascomycetous truffles and the true and false morels from 18S and 28S ribosomal DNA sequence analysis. Mycologia, 89, 48-65.
  35. O’Donnell, K., Cigelnik, E. & Nirenberg, H. I. (1998). Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia, 90, 465-493.
  36. O’Donnell, K., Humber, R. A., Geiser, D. M., Kang, S., Park, B., Robert, V. A. R. G., Crous, P. W., Johnston, P. R., Aoki, T., Rooney, A. P. & Rehner, S. A. (2012). Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycologia, 104(2), 427-445.
  37. Padasht, F. (1993). Evaluation of rice foot rot disease (Gibberella fujikuroi) in Guilan. M. Sc. thesis, University of Tehran. 123 pp. (in Farsi)
  38. Peterson, S. W. & Logrieco, A. (1991). Ribosomal RNA sequence variation among interfertile strains of some Gibberella spp. Mycologia, 83, 397-402.
  39. Petrovic, T., Burgess, L. W., Cowie, I., Warren, R. A. & Harvey, P. R. (2013). Diversity and fertility of Fusarium sacchari from wild rice (Oryza australiensis) in Northern Australia, and pathogenicity tests with wild rice, rice, sorghum and maize. European Journal of Plant Pathology, 136, 773-788.
  40. Pordel, A. (2014). Systematic study on Magnaporthe species in southern Caspian Sea. M.Sc. thesis. University of Tehran, Iran. 105 pp. (in Farsi)
  41. Safaee, D., Hedjaroude, Gh. A. & Okhovvat, M. (2000). The first report of presence of Fusarium merismoides and F. cf. udum in Iran. Proceedings of the 14th Iranian plant protection congress, vol. II, 5–8 Sept., Esfahan, Iran: 1.
  42. Safaee, D., Younesi, H. & Sheikholeslami, M. (2012). Fusarium species that cause root and crown rot of wheat in Kermanshah province. Iranian Journal of Plant Pathology, 48(2), 89 -91 (Short Article)
  43. Saponaro, A., Puglia, A. P. & Montorsi, F. (1986). Some important seedborn pathogenic fungi on rice. (Abstr.) Informatore Fitopatologico, 36(1), 40.
  44. Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A. & Chen, W. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. In: Proceedings of the National Academy Sciences of the United States of America, 109, 6241-6246.
  45. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731-2739.
  46. Triest, D., Cremer, K. D., Denis Piérard, D. & Hendrickx, M. (2016). Unique Phylogenetic Lineage Found in the Fusarium-like Clade after Re-examining BCCM/ IHEM Fungal Culture Collection Material. Mycobiology, 44(3), 121-130.
  47. Verstraete, F. (2008). EU legislation on mycotoxins in food and feed: overview of the decision-making process and recent and future developments. In Mycotoxins, Leslie, J.F., Bandyopadhyay, R. & Visconti, A., (eds.). CABI,Wallingford, Oxfordshire, UK., pp. 77-99.
  48. Wafa, S. & Mohamed Zubi. (2016). Occurrence and characterization of Fusarium spp. isolated from Mangrove soils. Ph.D. thesis, Universiti Sains Malaysia. 168 pp.
  49. Xu, J. R., Yan, K., Dickman, M. B. & Leslie, J. F. (1995). Electrophoretic karyotypes distinguish the biological species of Gibberella fujikuroi (Fusarium section Liseola). Molecular Plant-Microbe Interactions, 8, 74-84.
  50. Zhong, S. & Steffenson, B. J. (2001). Virulence and molecular diversity of Cochliobolus sativus. Phytopathology, 91, 469-476.
آمار
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