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حذف آنتیبیوتیک نورفلوکساسین از محلول آلوده بوسیله ریزجلبک کلرلا | ||
| تحقیقات آب و خاک ایران | ||
| مقاله 2، دوره 50، شماره 6، آبان 1398، صفحه 1307-1316 اصل مقاله (1.77 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2019.269195.668050 | ||
| نویسندگان | ||
| زهرا دارابی1؛ نسرین قربان زاده* 2؛ محمدباقر فرهنگی3؛ داود بخشی4 | ||
| 1دانشجوی کارشناسی ارشد، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 2استادیار، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 3استادیار،گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 4دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| چکیده | ||
| در این پژوهش توانایی حذف آنتیبیوتیک نورفلوکساسین از محلول آبی توسط ریزجلبک کلرلا مورد ارزیابی قرار گرفت. همچنین پیامدهای سمی این آنتیبیوتیک بر روی ریزجلبک کلرلا با مطالعه ویژگیهای رشدی و بیوشیمیایی آن شامل وزن خشک سلول، کلروفیل کل و محتوای کارتنوئید بررسی شد. آزمایش در قالب طرح کاملاً تصادفی با آرایش فاکتوریل و با سه تکرار انجام شد. فاکتورهای آزمایش شامل پنج سطح آنتیبیوتیک (صفر، 20، 50، 80، 120 میلیگرم بر لیتر) و شش زمان نمونهبرداری (صفر، 1، 3، 6، 9 و ۱۴ روز) بود. وزن خشک سلولی ریزجلبک با افزایش غلظت نورفلوکساسین به طور معنیداری کاهش یافت و در پایان ۱۴ روز انکوباسیون در غلظتهای ۵۰، ۸۰ و ۱۲۰میلیگرم بر لیتر آنتیبیوتیک در مقایسه با شاهد به ترتیب ۶1/21، 41/42 و 23/55 درصد کاهش نشان داد. مقادیر کلروفیل کل و کارتنوئید با افزایش زمان انکوباسیون به طور معنیداری به دلیل تنش ناشی از نورفلوکساسین افزایش پیدا کردند. غلظت مؤثر (EC50) نورفلوکساسین برای ریزجلبک کلرلا 71/453 میلیگرم بر لیتر در پایان 14 روز انکوباسیون محاسبه شد. کلرلا توانست پس از دو هفته انکوباسیون به ترتیب 15/69، 78/51، 41/28 و 18/18 درصد آنتیبیوتیک نورفلوکساسین را از محلول آبی دارای غلظتهای 20، 50، 80 و 120 میلیگرم بر لیتر حذف کند. | ||
| کلیدواژه‌ها | ||
| آلاینده‌های نوظهور؛ ریزجلبک؛ تجمع زیستی؛ فلوروکینولون | ||
| عنوان مقاله [English] | ||
| Removal of Norfloxacin Antibiotic from Contaminated Solution by Microalgae Chlorella sp. | ||
| نویسندگان [English] | ||
| Zahra Darabi1؛ Nasrin Ghorbanzadeh2؛ Mohammad Bagher Farhangi3؛ Davood Bakhshi4 | ||
| 1M.Sc. Student. Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Assistant Professor, Department of soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 3Assistant Professor, Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 4Associate Professor, Department of Horticulture Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| In this study, the removal potential of norfloxacin antibiotic from water was investigated using Chlorella sp. microalga. Also, the toxicological effects of norfloxacin on Chlorella sp. was examined by studying the growth and biochemical characteristics of the microalga including cell dry weight, total chlorophyll and carotenoid content. A multi-level factorial experiment with complete randomized design was performed with three replications. The experimental factors included 5 levels of antibiotic (0, 20, 50, 80 and 120 mg L-1) and 6 sampling times (0, 1, 3, 6, 9 and 14 day). The cell dry weight of Chlorella sp. was significantly reduced at increased concentrations of norfloxacin, showing 12.61, 42.41 and 55.23% reduction at 50, 80 and 120 mg L−1 norfloxacin, respectively, as compared to the control treatment after 14 days of incubation. The total chlorophyll and carotenoid contents were significantly increased by prolonging incubation time as a result of norfloxacine stress. The effective concentration (EC50) of norfloxacin was calculated to be 453.71 mg L−1 for Chlorella sp. at the end of day 14. Chlorella sp. was able to eliminate norfloxacine up to 69.15, 51.78, 28.41 and 18.18% from 20, 50, 80 and 120 mg L−1 water solutions, respectively, after 14 days of incubation. | ||
| کلیدواژه‌ها [English] | ||
| Emerging contaminants, Algea, Bioaccumulation, Fluoroquinolone | ||
| مراجع | ||
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