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بهینه‌سازی فرمولاسیون کود زیستی برای افزایش انحلال فسفر توسط قارچ آسپرژیلوس | ||
| تحقیقات آب و خاک ایران | ||
| مقاله 3، دوره 51، شماره 4، تیر 1399، صفحه 829-839 اصل مقاله (694.75 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2019.292967.668405 | ||
| نویسندگان | ||
| معصومه حسینی1؛ محسن برین* 2؛ میرحسن رسولی صدقیانی3؛ فرخ اسد زاده4 | ||
| 1دانشجوی کارشناسی ارشد، گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه | ||
| 2استادیار گروه علوم خاک- دانشگاه ارومیه | ||
| 3استاد گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه | ||
| 4دانشیار گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه | ||
| چکیده | ||
| فسفر یکی از عناصر غذایی پرمصرف بوده که کمبود آن رشد گیاه را به شدت محدود میکند. یکی از سادهترین و کم هزینهترین روشهای تأمین فسفر گیاه کاربرد مستقیم خاکفسفات می باشد اما در خاکهای آهکی به علت حلالیت ناچیز آن چندان موثر نیست. استفاده از خاکفسفات مخلوطشده با گوگرد و مواد آلی به همراه ریزجانداران حلکنندههای فسفات از جمله این راهکارها محسوب میشوند. این مطالعه با هدف مدلسازی بررسی تأثیر نسبتهای مختلف ورمیکمپوست، خاکفسفات و گوگرد بر میزان انحلال و آزادسازی فسفر توسط قارچ آسپرژیلوس و ارائه سطوح مطلوب این متغیرها برای تهیه کود زیستی کارآمد انجام شد. بر این اساس تعداد 20 آزمایش با استفاده از روش سطح پاسخ بر مبنای طرح مرکب مرکزی تعریف شد و اثر مقادیر مختلف متغیرهای ورمیکمپوست، خاکفسفات و گوگرد و به صورت کدبندی شده در محدودهی (1+، 0، 1-) بر میزان انحلال فسفر بررسی شد. نتایج نشاندهنده کارآمدی بالای (8841/0= R2) مدل طرح مرکب مرکزی در برآورد انحلال فسفر بود. بر اساس نتایج، برهمکنش ورمیکمپوست با گوگرد ( 05/0>p ) و بر همکنش خاکفسفات با گوگرد ( 05/0>p ) معنیدار بود. نتایج تحلیل آماری ضرایب مدل طرح مرکب مرکزی حاکی از اثر مثبت و فزاینده ورمیکمپوست، ورمیکمپوست × گوگرد و خاکفسفات × گوگرد بر افزایش انحلال فسفر می باشد. بر اساس پیشبینی شرایط بهینه برای انحلال فسفر، مقادیر 58 درصد ورمیکمپوست، 3/23 درصد خاکفسفات و 7/18 درصد گوگرد منجر به ماکزیمم انحلال فسفر (04/773 میلیگرم بر کیلوگرم) توسط قارچ آسپرژیلوس در کود میکروبی میشود. | ||
| کلیدواژه‌ها | ||
| مدلسازی؛ قارچ حل‌کننده فسفر؛ طرح مرکب مرکزی؛ کود زیستی | ||
| عنوان مقاله [English] | ||
| Optimization of Biofertilizer Formulation for Increasing Phosphorus Solubility by Aspergillus Fungus | ||
| نویسندگان [English] | ||
| masomeh Hoseini1؛ Mohsen Barin2؛ MirHassan Rasouli-Sadaghiani3؛ Farrokh Asadzadeh4 | ||
| 1MSc Student, Soil Science Department, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 2Assist. Prof., Soil Science Department, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Prof. Soil Science Department, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 4Assoc. Prof., Soil Science Department, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده [English] | ||
| Phosphorus is one of the macronutrient that its deficiency severely restricts plant growth. One of the simplest and least costly methods of providing phosphorus is direct application of rock phosphate but in calcareous soils it is not very effective due to its low solubility. The use of rock phosphate mixed with sulfur and organic matter along with phosphate solubilizing microorganisms is considered as a method for increasing rock phosphorus solubility. This study aimed to model the effect of different ratios of vermicompost, rock phosphate and sulfur on dissolution and release of phosphorus by Aspergillus sp and to optimize the levels of these variables for efficient biofertilizer preparation. Accordingly, 20 experiments were designed using response surface methodology based on central composite design. The effects of different values of vermicompost, rock phosphate and sulfur variables encoded in the constraint (+1, 0, -1) on the dissolution rate of phosphorus were modeled. The results showed a high efficiency (R2 = 0.8841) of the central composite design model in estimating P dissolution. The results also indicated that vermicompost interaction with sulfur (p <0.05) and interaction of rock phosphate with sulfur (p <0.05) were significant. The results of the statistical analysis of the central composite model coefficients indicated that the vermicompost, vermicompost*sulfur and rock phosphate*sulfur additives had a positive and incremental effect on the phosphorus solubility. According to prediction of optimum conditions for phosphorus solubilization, 58% vermicompost, 23.3% rock phosphate and 18.7% sulfur resulted in maximum phosphorus solubilization (773.04 mg / kg) by Aspergillus sp. in microbial fertilizer. | ||
| کلیدواژه‌ها [English] | ||
| modelling, phosphate solubilizing fungus, central composite design, biofertilizer | ||
| مراجع | ||
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