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ارزیابی اثر آنتاگونیستی استرپتومایسس های ریزوسفر گندم در بیوکنترل پوسیدگی معمولی ریشه ناشی از Bipolaris sorokiniana | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
مقاله 10، دوره 8، شماره 2، اردیبهشت 1399، صفحه 113-126 اصل مقاله (816.89 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2019.281475.264 | ||
نویسندگان | ||
رسول اکبرپور1؛ کیوان بهبودی* 2 | ||
1گیاهپزشکی، کشاورزی، تهران، کرج، ایران | ||
2عضو هیئت علمی دانشگاه تهران | ||
چکیده | ||
پوسیدگی معمولی ریشه و طوقه گندم با عامل Bipolaris sorokiniana از مهمترین بیماریهای گندم در جهان است. در این مطالعه 60 جدایه استرپتومایسس از ریزوسفر گندم جداسازی و به روش کشت متقابل در شرایط آزمایشگاه غربال شدند. جدایههای UTS22، UTS3 و UTS4 با 6/46، 6/45 و 42 درصد بیشترین میزان ممانعت را از رشد میسلیوم بیمارگر نشان دادند. اغلب جدایههای مورد بررسی به فعالیتهای فیزیولوژیکی و آنزیمی نظیر کلنیزاسیون، تثبیت نیتروژن، حلالیت فسفات، آنزیم پروتئاز و کیتیناز نتیجه مثبت نشان دادند. به ترتیب جدایههای UTS3، UTS18 و UTS4 با 96/67، 3/61 و 63/54 درصد بیشترین میزان ممانعت را با تولید متابولیتهای فرار از رشد میسلیوم بیمارگر نشان دادند. بررسیهای گلخانهای در قالب طرح کامل تصادفی با استفاده از روش پوششدار کردن بذر گندم با غلظت CFU/ml 108 سوسپانسیون جدایههای برتر انجام شد. بر اساس نتایج، جدایه UTS22 با 6/55 درصد بیشترین میزان کنترل بیماری را نشان داد و همچنین باعث افزایش معنیدار شاخصهای رشد گندم در مقایسه با تیمار شاهد سالم شد. با توجه به نتایج شناسایی مولکولی بر اساس توالی 16S rDNA، جدایه UTS22 متعلق به گونه Streptomyces fulvissimus بوده و به عنوان جدایه برتر در کنترل بیولوژیک B . sorokiniana معرفی میشود. | ||
کلیدواژهها | ||
گندم؛ کنترل بیولوژیک؛ Bipolaris sorokiniana؛ استرپتومایسس؛ کلنیزاسیون | ||
عنوان مقاله [English] | ||
Evaluation of antagonistic effect of wheat rhizosphere Streptomyces on biocontrol of common root rot caused by Bipolaris sorokiniana | ||
نویسندگان [English] | ||
Rasoul Akbarpour1؛ keyvan behbodi2 | ||
1Plant medicine, Agriculture, Tehran, Karaj, Iran | ||
2Faculty Member of Tehran University | ||
چکیده [English] | ||
Common root and crown rot of wheat caused by Bipolaris sorokiniana is one of the most important diseases of wheat in the world. In this study, 60 isolates of Streptomyces were isolated from wheat rhizosphere and screened in laboratory condition using dual culture. UTS22, UTS3 and UTS4 isolates showed maximum inhibition with 46.6, 45.6 and 42% respectively. Most of the isolates had positive reaction for physiological and enzymatic activities such as colonization, nitrogen fixation, phosphate solubility, protease and chitinase. UTS3, UTS18 and UTS4 isolates showed the highest inhibition levels of pathogenic mycelial growth by producing metabolites with 67.96, 61.3 and 54.63% respectively. Greenhouse studies carried out in a completely randomized design with wheat seed coat method with 108 CFU/ml concentrations of supernatant isolates. Based on the results, the UTS22 isolate showed the highest disease control with 55.6% and also wheat growth indices increased significantly compared to healthy control treatment. Molecular identification based on the sequence of 16S showed, the UTS22 isolate belongs to Streptomyces fulvissimus. UTS22 is introduced as a superior isolate in biological control of B. sorokiniana. | ||
کلیدواژهها [English] | ||
Wheat, Biological control, Bipolaris sorokiniana, Streptomyces, Colonization | ||
مراجع | ||
Acharya K, Dutta AK, Pradhan P (2011) Bipolaris sorokiniana (Sacc.) Shoem: the most destructive wheat fungal pathogen in the warmer areas. Australian Journal of Crop Science 5(9): 1064–1071. Baldani VLD, Döbereiner J (1980) Host-plant specificity in the infection of cereals with Azospirillum spp. Soil biology and biochemistry 12(4): 433-439. Brizzio S, Turchetti B, De Garcia V, Libkind D, Buzzini P, Van Broock M (2007) Extracellular enzymatic activities of basidiomycetous yeasts isolated from glacial and subglacial waters of northwest Patagonia (Argentina). Canadian Journal of Microbiology 53(4): 519-525. Buzzini P, Martini A (2002) Extracellular enzymatic activity profiles in yeast and yeast‐like strains isolated from tropical environments. Journal of Applied Microbiology 93(6): 1020-1025. Chater KF, Biro S, Lee KJ, Palmer T, Schrempf H (2010) The complex extracellular biology of Streptomyces. FEMS Microbiology Reviews 34(2): 171-198. Delcour J, Hoseney RC (2010) Principles of cereal science and technology authors provide insight into the current state of cereal processing. Cereal Foods World 55(1): 21-22. Deshpande M (1986) Enzymatic degradation of chitin and its biological applications. Journal of Scientific and Industrial Research 45(6): 273- 281. Eden PA, Schmidt TM, Blakemore RP, Pace NR (1991) Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rRNA-specific DNA. International Journal of Systematic and Evolutionary Microbiology 41(2): 324-325. El-Tarabily KA (2008) Promotion of tomato (Lycopersicon esculentum Mill.) plant growth by rhizosphere competent 1-aminocyclopropane-1-carboxylic aciddeaminase-producing streptomycete actinomyc. Plant and Soil 308(1-2): 161-178. Gangwar M, Dogra S, Gupta UP, Kharwar RN (2014) Diversity and biopotential of endophytic actinomycetes from three medicinal plants in India. African Journal of Microbiology Research 8(2): 184-191. Gopalakrishnan S, Vadlamudi S, Bandikinda P, Sathya A, Vijayabharathi R, Rupela O, Varshney RK (2014) Evaluation of Streptomyces strains isolated from herbal vermicompost for their plant growth promotion traits in rice. Microbiological Research 169(1):40-48. Gupta PK, Chand R, Vasistha N, Pandey SP, Kumar U, Mishra VK, Joshi AK (2018) Spot blotch disease of wheat: the current status of research on genetics and breeding. Plant pathology 67(3): 508-531. Hamdali H, Bouizgarne B, Hafidi M, Lebrihi A, Virolle MJ, Ouhdouch Y (2008) Screening for rock phosphate solubilizing actinomycetes from Moroccan phosphate mines. Applied Soil Ecology 38(1): 12-19. Haran S, Schickler H, Chet I (1996) Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum. Microbiology 142(9): 2321-2331. Ibanez F, Taurian T, Angelini J, Tonelli ML, Fabra A (2008) Rhizobia phylogenetically related to common bean symbionts Rhizobium giardinii and Rhizobium tropici isolated from peanut nodules in Central Argentina. Soil Biology and Biochemistry 40(2): 537-539. Ja G (2003) Candicidin biosynthesis in Streptomyces griseus. Applied and Environmental Microbiology 60(3): 633-642. Jog R, Pandya M, Nareshkumar G, Rajkumar S (2014) Mechanism of phosphate solubilization and antifungal activity of Streptomyces spp. isolated from wheat roots and rhizosphere their application in improving plant growth. Microbiology 160(4): 778-788 Júnior AG, dos Santos ÁF, Auer CG (2000) Perspectivas do uso do controle biológico contra doenças florestais. Floresta 30(1-2): 155-166. Kennedy IR, Pereg-Gerk L, Wood C, Deaker R, Gilchrist K, Katupitiya S (1997) Biological nitrogen fixation in non-leguminous field crops: facilitating the evolution of an effective association between Azospirillum and wheat. Plant and Soil 194(1-2): 65-79. Khamna S, Yokota A, Lumyong S (2009) Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World Journal of Microbiology and Biotechnology 25(4): 649-655. Khayat maher R, Amoozegar MA, Seyyedmahdi SH, Hamedi J, Naghavi MR, Foroozanfar F, Latifi AM (2012) Isolation and screening of phytotoxin producing actinomycetes and determination of phytotoxin effect spectrum of selected strains. Biological Journal of Microorganism 1(2): 1- 22. Kraus L, Loper JE (1992) Lack of evidence for a role of antifungal metabolite production by Pseudomonas fluorescens Pf-5 in biological control of Pythium damping-off of cucumber. Phytopathology 82(3): 264-271. Kreuze JF, Suomalainen S, Paulin L, Valkonen JP (1999) Phylogenetic analysis of 16S rRNA genes and PCR analysis of the nec1 gene from Streptomyces spp. causing common scab, pitted scab, and netted scab in Finland. Phytopathology 89(6): 462-469. Ledingham RJ, Atkinson TG, Horricks JS, Mils JT, Piening LJ, Tinline RD (1973) Wheat losses due to common root rot in the prairies provinces of Canada 1969-1971. Canadian Plant Disease 53(3): 113-122. Ling N, Xue C, Huang Q, Yang X, Xu Y, Shen Q (2010) Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt. Biocontrol 55(5): 673-683. Monteiro P, Borba MP, Van Der Sand ST (2017) Evaluation of the antifungal activity of Streptomyces sp. on Bipolaris sorokiniana and the growth promotion of wheat plants. Journal of Agricultural Science 9(12): 229-240. Nguyen XH, Naing KW, LeeYS, Tindwa H, Lee GH, Jeong BK, Ro HM, Kim SJ, Jung WJ, Kim KY (2012) Biocontrol potential of Streptomyces griseus H7602 against root rot disease (Phytophthora capsici) in pepper. The Plant Pathology Journal 28(3): 282-289. Olano C, Lombo F, Mendez C, Salas JA (2008) Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering. Metabolic Engineering 10(5): 281-292. Oliveira MF, da Silva MG, Van Der Sand ST (2010) Anti-phytopathogen potential of endophytic actinobacteria isolated from tomato plants (Lycopersicon esculentum) in southern Brazil, and characterization of Streptomyces sp. R18 (6), a potential biocontrol agent. Research in Microbiology 161(7): 565-572. Palaniyandi SA, Yang SH, Zhang L, Suh JW (2013) Effects of actinobacteria on plant disease suppression and growth promotion. Applied Microbiology and Biotechnology 97(22): 9621-9636. Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and Environmental Microbiology 68(8): 3795-3801. Pieterse CM, Leon-Reyes A, Van derent S, Van Wees SC (2009) Networking by small-molecule hormones in plant immunity. Nature Chemical Biology 5(5): 308-316. Queiroz BPVD, Aguilar-Vildoso CI, Melo IS (2006) Visualização in vitro da colonização de raízes por rizobactérias. Summa Phytopathologica 32(1): 95-97. Raza W, Faheem M, Yousaf S, Rajer FU, Yamin M (2013) Volatile and nonvolatile antifungal compounds produced by Trichoderma harzianum SQR-T037 suppressed the growth of Fusarium oxysporum f. sp. niveum. Science Letter 1(1): 21-24. Singh PJ, Mehrotra RS (1980) Biological control of Rhizoctonia bataticola on gram by coating seed with Bacillus and Streptomyces spp. and their influence on plant growth. Plant andSoil 56(3): 475-483. Sperber JI (1958) The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural Research 9(6): 778-781. Tinline RD (1977) Multiple infections of subcrown internodes of wheat (Triticum aestivum) by common root rot fungi. Canadian Journal of Botany 55(1): 30-34. Wiese MV (1987) Compendium of wheat diseases. American Phytopathological Society. APS Press.122pp. Wildermuth GB, McNamara RB (1987) Susceptibility of winter and summer crops to root and crown infection by Bipolaris sorokiniana. Plant pathology 36(4): 481-491. Zhang J, Wang JD, Liu CX, Yuan JH, Wang XJ, Xiang WS (2014) A new prenylated indole derivative from endophytic actinobacteria Streptomyces sp. neau-D50. Natural ProductResearch 28(7): 431-437.
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