پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 161 |
| تعداد شماره‌ها | 6,532 |
| تعداد مقالات | 70,501 |
| تعداد مشاهده مقاله | 124,111,821 |
| تعداد دریافت فایل اصل مقاله | 97,215,420 |
جداسازی و استفاده از باکتریهای تولیدکننده اورهآز و ال-آسپاراژیناز موثر در تولید زیستی کربنات کلسیم به منظور حذف روی از محلولهای آلوده | ||
| تحقیقات آب و خاک ایران | ||
| دوره 52، شماره 2، اردیبهشت 1400، صفحه 549-562 اصل مقاله (995.99 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2020.313712.668802 | ||
| نویسندگان | ||
| زهرا قنبری1؛ نسرین قربان زاده* 2؛ محمدباقر فرهنگی1؛ مریم خلیلی راد3 | ||
| 1ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رﺷﺖ، اﯾﺮان | ||
| 2استادیار ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه علوم ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رشت، ایران | ||
| 3ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رشت، ایران | ||
| چکیده | ||
| آلودگی منابع خاک و آب به فلزهای سنگین نه تنها در تولید محصولات کشاورزی سالم بلکه در سلامت اکوسیستم نیز به یک مسئله جدی تبدیل شده است. فرایند رسوب کربنات کلسیم تحریک شده میکروبی یک روش کمهزینه و سازگار با محیط زیست در راستای کاهش آلودگی منابع آب و خاک است. هدف این پژوهش جداسازی باکتریهای بومی و موثر در تولید زیستی کربنات کلسیم به منظور حذف فلز روی از محلولهای آلوده بود. غربالگری و جداسازی باکتریهای بومی تولیدکننده اورهآز و ال-آسپاراژیناز انجام شد و سپس تغییرات آمونیاک، pH و قابلیت هدایت الکتریکی و همچنین حذف روی از محلول آلوده با کاربرد دو باکتری جدا شده در حضور باکتری شاخص Sporosarcinapasteuriiمورد مطالعه قرار گرفت. نتایج نشان داد که در حضور هر سه باکتری مقدار آمونیاک تولید شده، pH و قابلیت هدایت الکتریکی نسبت به شاهد (بدون مایهزنی باکتری) افزایش معنیداری پیدا کرد (p ≤0.01). کارایی جدایه تولیدکننده اورهآز جدا شده در حذف روی از محلول آلوده تقریباً به اندازه کارایی باکتری Sporosarcinapasteuriiبود اما کارایی جدایه تولیدکننده ال-آسپاراژیناز بومی بالاتر از آنها بود. Sporosarcinapasteuriiحذف 32/51، 94/65، 36/70 درصدی و جدایه تولیدکننده اورهآز حذف 49/65 و 07/68 و 46/71 درصدی را نسبت به مقدار اولیه روی بهترتیب در غلظتهای 5/0، 2 و 4 میلیمولار نشان دادند و جدایه تولیدکننده ال-آسپاراژیناز در غلظتهای 5/0، 2، 4 و 8 میلیمولار روی بهترتیب 29/96، 88/93، 06/97 و 32/97 درصد روی را حذف نمود. بنابراین به نظر میرسد باکتریهای بومی تولیدکننده اورهآز و ال-آسپاراژیناز میتوانند در زیستپالایی روی از محیطهای آبی آلوده با فرایند رسوب کربنات کلسیم تحریک شده میکروبی مفید و کارآمد باشند. | ||
| کلیدواژه‌ها | ||
| زیستپالایی؛ فلزهای سنگین؛ کلسیت؛ معدنی شدن زیستی | ||
| عنوان مقاله [English] | ||
| Isolating and Using Bacteria, Producing Urease and L-asparaginase, and Effective on Calcium Carbonate Bioproduction to Remove Zinc from Contaminated Solutions | ||
| نویسندگان [English] | ||
| Zahra Ghanbari1؛ Nasrin Ghorbanzadeh2؛ Mohammad Bagher Farhangi1؛ Maryam Khalili Rad3 | ||
| 1Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Assistant Professor, Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 3Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| Heavy metal pollution in soil and water resources has become a serious problem not only in the production of healthy agricultural products, but also in the ecosystem health. Microbially induced calciumcarbonate precipitation (MICP) is a low-cost and environmentally friendly methods for reducing water resources and soil pollution. The aim of this study was to isolate native and efficient bacteria in the biological production of calciumcarbonate in order to remove zinc from contaminated solution. Isolating and screening native bacteria, producing urease and L-asparaginase, was accomplished. Then, the changes in ammonia, pH and electrical conductivity (EC), as well as removal of zinc from the contaminated solutions were studied using these two efficient isolated bacteria in the presence of sporocarsina pasteurii. The results showed that in the presence of all three bacteria, the amount of produced ammonia, pH and EC in the culture media increased significantly compared to the ones in the control (without bacterial inoculation) (p < /em>≤0.05). The efficiency of isolated urease-producing strain in removal of zinc from the contaminated solution was almost equal to that of sporosarcina pasteurii, while the efficiency of isolated L-asparaginase-producing strain was more. Sporosarsina pasteurii removed 51.32, 65.94 and 70.36% and urease producing strain removed 65.49, 68.07, and 71.46 of zinc in the solutions containing 0.5, 2 and 4 mM Zn, respectively. However, L-asparaginase-producing strain removed 96.29, 93.88, 97.06 and 97.32% of zinc in solution containing 0.5, 2, 4 and 8 mM Zn, respectively. Therefore, it seems native urease- and L-asparaginase-producing bacteria can be useful and efficient in Zn bioremediation of contaminated solutions by MICP process. | ||
| کلیدواژه‌ها [English] | ||
| Biomineralization, Bioremediation, Calcite, Heavy metals | ||
| مراجع | ||
|
Achal, V., Mukherjee, A., Basu, P.C., and Reddy, M.S. (2009). Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. Journal of Industrial Microbiology and Biotechnology, 36(7), pp.981-988. Adriano, D.C. (2003). Trace Elements in Terrestrial Environments: Biogeochemistry, Bioavailability and Risks of Metals, Springer, New York, NY, USA, 2nd edition. Akiyama, M., and Kawasaki. S. (2012). Microbially mediated sand solidification using calcium phosphate compounds. Engineering Geology, 137-138: 29-39. Al-Thawadi, S.M. (2008). High Strength In-Situ Biocementation of Soil by Calcite Precipitating Locally Isolated Ureolytic Bacteria. PhD thesis. University of Murdoch, Western Australia. Al-Thawadi, S.M. (2011). Ureolytic bacteria and calcium carbonate formation as a mechanism of strength enhancement of sand. Journal of Advanced Science and Engineering Research 1: 98-114. Amoozegar, M.A., Ghazanfari, N., and Didari, M. (2012). Lead and cadmium bioremoval by Halomonas sp., an exopolysaccharide-producing halophilic bacterium. Progress in Biological Sciences, 2(1): 1-11. Anbu, P., Kang, C.H., Shin, Y.J., and So, J.S. (2016) .Formations of calcium carbonate minerals by bacteria and its multiple applications. Springerplus, 5: 250-262. Arima, K., Sakamoto, T., Araki, C., and Tamura, G., (1972). Production of extracellular L-asparaginases by microorganisms. Agricultural and Biological Chemistry, 36: 356-361. Bergey, D.H. (1984) Bergey’s Manual of Systematic Bacteriology. Williams and Wilkins, USA. Bhattacharya, A., Naik, S.N., and Khare, S.K. (2019). Efficacy of ureolytic Enterobacter cloacae EMB19 mediated calcite precipitation in remediation of Zn (II). Journal of Environmental Science and Health, Part A, 54(6), pp.536-542. Bruins, M.R., KapilS, O., and ehme, F.W. (2000). Microbial resistance to metals in the environment. Ecotoxicology and Environmental Safety, 45: 198–207. Burbank, M.B., Weaver, T.J., Williams, B.C., and Crawford, R.L. (2012). Urease activity of ureolytic bacteria isolated from six soils in which calcite was precipitated by indigenous bacteria. Geomicrobiology Journal, 29(4), pp.389-395. Castro-Alonso, M.J., Montañez-Hernandez, L.E., Sanchez-Muñoz, M.A., Macias Franco, M.R., Narayanasamy, R., and Balagurusamy, N. (2019). Microbially induced calcium carbonate precipitation (MICP) and its potential in bioconcrete; microbiological and molecular concepts. Frontiers in Materials, 6(126): 1-15. Christensen, W.B. (1946). Urea decomposition as a means of differentiating proteus and paracolon cultures from each other and from Salmonella and Shigella types. Journal of Bacteriology, 52: 461-466. Dhami, N.K., Reddy, M.S., and Mukherjee, A. (2014). Application of calcifying bacteria for remediation of stones and cultural heritages. Frontiers in microbiology, 5: 304. Dixit, R., Wasiullah, Malaviya, D., Pandiyan, K., Singh, U., Sahu, A., Shukla, R., Singh, B., Rai, J., Sharma, P., Lade, H., and Paul, D. (2015). Bioremediation of heavy metals from soil and aquatic environment: An overview of principles and criteria of fundamental processes. Sustainability, 7(2):2189. Fujita, Y., Ferris, F.G., Lawson, R.D., Colwell, F. S., and Smith, R. W. (2000). Calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicrobiology Journal, 17(4), pp. 305-318. Ganendra, G., De Muynck, W., Ho, A., Arvaniti, E.C., Hosseinkhani, B., Ramos, J.A., Rahier, H., and Boon, N., (2014). Formate oxidation driven calcium carbonate precipitation by Methylocystis parvus OBBP. Applied and Environmental Microbiology. 80:4659-466. Greany, K.M. (2005). An assessment of heavy metal contamination in the marine sediments of Las Perlas Archipelago, Gulf of Panama. M.S. thesis, School of Life Sciences Heriot-Watt University, Edinburgh, Scotland. Gulati, R., Saxena, R., and Gupta, R., (1997). A rapid plate assay for screening L- asparaginase producing microorganisms. Letters in Applied Microbiology, 24(1): 23-26. Hammad, I.A., Talkhan, F.N., and Zoheir, A.E. (2013). Urease activity and induction of calcium carbonate precipitation by Sporosarcina pasteurii NCIMB 8841. Journal of Applied Sciences Research, 9(3): 1525-1533. Hasan, M., Begum, L., Hosain, S., Poddar, P., Chowdhury, A., and Ali, F. (2017). Study on heavy metals (Zinc and Lead) in drinking water of tannery area, adjacent areas and outside village areas. Journal of Environmental and Analytical Toxicology, 7: 2. Hester, L.L., Sarvary, M.A., and Ptak, C.J. (2014). Mutation and selection: An exploration of antibiotic resistance in Serratia marcescens. Proceedings of the Association for Biology Laboratory Education, 35,140-183. Jalilvand, N., Akhgar, A., Alikhani, H.A., Asadi Rahmani, H., and Rejali, F. (2019). Removal of heavy metals zinc, lead, and cadmium by biomineralization of urease-producing bacteria isolated from Iranian mine calcareous soils. Journal of Soil Science and Plant Nutrition, 20(1): 10.1007/s42729-019-00121-z. Kang, C.H., Han, S.H., Shin, Y., Oh, S.J., and So, J.S. (2014). Bioremediation of Cd by microbially induced calcite precipitation. Applied Biochemistry and Biotechnology, 172(4): 1929-1937. Kang, C.H., and So, J.S. (2016). Heavy metal and antibiotic resistance of ureolytic bacteria and their immobilization of heavy metals. Ecological Engineering. 97: 304-312. Kirpichtchikova, T. A., Manceau, A., Spadini, L., Panfili, F., Marcus, M.A., and Jacquet, T. (2006). Speciation and solubility of heavy metals in contaminated soil using X-ray microfluorescence, EXAFS spectroscopy, chemical extraction, and thermodynamic modeling. Geochimica et Cosmochimica Acta, 70(9): 2163-2190. Kwon, H.K., Jeon, J.Y., and Oh, S.J. (2017). Potential for heavy metal (copper and zinc) removal from contaminated marine sediments using microalgae and light emitting diodes. Ocean Science Journal, 52, 57 –66. Leboffe, M.J. and Pierce, B.E. (2015). Microbiology: laboratory theory and application. Morton Publishing Company. Li, M., Chen, G.X., and Guo, H. (2013). Heavy metal removal by biomineralization of urease producing bacteria isolated from soil. International Biodeterioration and Biodegradation, 76: 81–85. Li, M., Fu, Q.L., Zhang, Q., Achal, V., and Kawasaki, S. (2015). Bio-grout based on microbially induced sand solidification by means of asparaginase activity. Scientific Reports, 5(16128):1-9. Mitchell, A.C., and Ferris, F.G. (2005). The coprecipitation of Sr into calcite precipitates induced by bacterial ureolysis in artificial groundwater: temperature and kinetics dependence, Geochim Gosmochim Acta, 69: 4199–4210. Moberly, J., Staven, A., Sani, R., and Peyton, B. (2010). Influence of pH and inorganic phosphate on toxicity of zinc to Arthrobacter sp. isolated from heavy-metal-contaminated sediments. Environmental Science and Technology, 44: 7302-7308. Mugwar, A.J., and Harbottle, M.J. (2016). Toxicity effects on metal sequestration by microbially-induced carbonate precipitation. Journal of Hazardous Materials, 314: 237-248. Peleg, M., and Corradini, M.G. (2011). Microbial growth curves: what the models tell us and what they cannot. Critical Reviews in Food Science and Nutrition is a food science journal, 51(10): 917-945. Redmile-Gordon, M., and Chen, L. (2017). Zinc toxicity stimulates microbial production of extracellular polymers in a copiotrophic acid soil. International Biodeterioration and Biodegradation, 119: 413-418. Ruggiero, C.E., Boukhalfa, H., Forsythe, J.H., Lack, J.G., Hersman, L.E., and Neu, M.P. (2005). Actinide and metal toxicity to prospective bioremediation bacteria. Environmental Microbiology, 7(1): 88-97. Sarada, D., Choonia, H.S., Sarode, D.D., and Lele, S.S. (2009). Biocalcification by Bacillus pasteurii urease: a novel application. Journal of Industrial Microbiology and Biotechnology, 36: 1111-1115. Singh, R., Gautam, N., Mishra, A., and Gupta, R. (2011). Heavy metals and living systems: An overview. Indian Journal of Pharmacology, 43(3): 246-253. Stocks-Fischer, S., Galinat, J.K. and Bang, S.S. (1999). Microbiological precipitation of CaCO3. Soil Biology and Biochemistry, 31: 1563-1571. Tobler, D.J., Cuthbert, M.O., Greswell, R.B., Riley, M.S., Renshaw, J.C., Handley-Sidhu, S., and Phoenix, V.R. (2011). Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite. Geochimica et Cosmochimica Acta, 75(11): 3290-3301. Whiffin, V.S. (2004). Microbial CaCO3 precipitation for the production of biocement. Ph.D. dissertation, University of Murdoch. Western Australia. Yamagata, H., Yoshizawa, M., and Minamiyama, M. (2010). Assessment of current status of zinc in wastewater treatment plants to set effluent standards for protecting aquatic organisms in Japan. Environmental Monitoring and Assessment, 169: 67–73. Yamina, B., Tahar, B., and Laure, F. M. (2012). Isolation and screening of heavy metal resistant bacteria from wastewater: a study of heavy metal co-resistance and antibiotics resistance. Water Science and Technology, 66: 2041–2048. Yu, X., Qian, C., Xue, B., and Wang, X. (2015). The influence of standing time and content of the slurry on bio-sandstone cemented by biological phosphates. Construction and Building Materials, 82: 167-172. Zhao, Y., Yao, J., Yuan, Z., Wang, T., Zhang, Y., and Wang, F. (2016). Bioremediation of Cd by strain GZ-22 isolated from mine soil based on biosorption and microbially induced carbonate precipitation. Environment Scienece and Pollutant Research, 24(1): 372-380. | ||
|
آمار تعداد مشاهده مقاله: 693 تعداد دریافت فایل اصل مقاله: 415 |
||