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بررسی توان برخی جدایههای باکتریایی به منظور مهار زیستی بیماری لکه زاویهای باکتریایی خیار | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
دوره 9، شماره 2، بهمن 1399، صفحه 127-141 اصل مقاله (949.86 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2021.323742.303 | ||
نویسندگان | ||
سیدزمان حسینی1؛ پژمان خدایگان* 2؛ روح اله صابری3 | ||
1دانشجوی دکتری گروه گیاهپزشکی دانشگاه ولی عصر (ع) رفسنجان- | ||
2عضو هیات علمی گروه گیاهپزشکی دانشگاه ولی عصر رفسنجان | ||
3عضو هیات علمی گروه گیاهپزشکی دانشگاه ولی عصر رفسنجان | ||
چکیده | ||
باکتریهای محرک رشد گیاهان با داشتن توان بالا در جهت افزایش زیستتوده و کنترل بیماریهای گیاهی، میتوانند جانشین مناسبی برای سموم و کودهای شیمیایی در کشاورزی باشند. بهمنظور بررسی مهار زیستی Pseudomonas syringae pv. lachrymans عامل بیماری لکه زاویهای خیار در شرایط آزمایشگاه و گلخانه، از باکتریهای جداسازی شده از خاک ریزوسفر خیار به همراه تعدادی از نمونههای مجموعه باکتریهای گروه گیاهپزشکی دانشگاه ولیعصر رفسنجان استفاده شد. بعد از غربالگری آزمایشگاهی، چهار جدایه که دارای توان ایجاد هاله بازدارنده بودند برای بررسیهای بعدی در شرایط آزمایشگاه و گلخانه انتخاب شدند. خصوصیات مهار زیستی جدایهها مانند تولید متابولیتهای ثانویه و بعضی از ویژگیهای تحریککنندگی رشد در شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. در ارزیابیهای گلخانهای، توان جدایههای باکتریایی در کنترل بیماری از طریق ممانعت در ایجاد علایم با تعیین سطح زیر منحنی پیشرفت بیماری و افزایش زیستتوده گیاه خیار با بررسی شاخصهای رشدی مطالعه شد. نتایج بررسی گلخانهای نشان داد که جدایه Pseudomonas fluorescens VRPF 54 کارآیی مناسبی در مهار زیستی بیماری داشت. در شرایط گلخانه، این جدایه به تنهایی و تواًم با بیمارگر به طور معنیداری باعث افزایش زیستتوده و کنترل بیماری لکه زاویهای خیار گردید. پس از انتخاب این جدایه، برخی از ویژگیهای آنتاگونیستی، خصوصیات بیوشیمیایی و شناسایی مولکولی آن در آزمایشگاه مورد بررسی قرار گرفت. همچنین نتایج حاصل از اندازهگیری آنزیمهای پراکسیداز، گایاکولپراکسیداز، فنیلآلانینآمونیالیاز، پلیفنلاکسیداز و محتوی فنل کل روند افزایشی را نشان داد. | ||
کلیدواژهها | ||
PGPR؛ کنترل بیولوژیک؛ سودوموناس فلورسنت | ||
عنوان مقاله [English] | ||
Evaluation of the potential of some bacterial isolates for biological control of angular leaf spot disease of cucumber | ||
نویسندگان [English] | ||
seyed zaman hosseini1؛ Pejman Khodaygan2؛ Roohallah Saberi riseh3 | ||
1Ph.D. Candidate, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan. | ||
2Department of plant protection, faculty of agriculture | ||
3Department of plant protection faculty of agriculture vali-e-asr university of rafsanjan | ||
چکیده [English] | ||
Plant growth-promoting rhizobacteria have great potential for increasing plant biomass and controlling plant diseases. Therefore PGPRs can be good substitute for the use of pesticides and chemical fertilizers in agriculture. For evaluation of the effects of antagonistic activities of several PGPRs against Pseudomonas syringae pv. lachrymans the causal agent of cucumber angular leaf spot disease in laboratory and greenhouse Conditions. Several bacteria isolated from different cucumber rhizosphere soil in Kerman province and a number of bacterial strains in the collection of Vali-e-Asr University of Rafsanjan were used. After in vitro screening, four isolates with ability of growth inhibitory effect were selected for further studies in vitro and in planta. Antibacterial activity characteristics of biocontrol agents such as production of secondary metabolites and some of their plant growth promoting traits were evaluated in vitro. During in planta study, the ability of bacterial strains to prevent the development of symptoms using the area under disease progress curve (AUDPC) and increasing the biomass of cucumber plant were evaluated. Greenhouse study showed that Pseudomonas fluorescens VRPF 54 strain have high biocontrol efficacy. During in planta study, this strain, both alone and in the interaction plant-pathogen in comparison with the control plant, significantly increased biomass and controlled cucumber leaf spot disease. After selection of this strain, some its antagonistic activities, biochemical properties and molecular identification were screened in vitro. Also, the assay of antioxidant enzymes activity such as peroxidase, guaiacol peroxidase, phenylalanine ammonia lyase, polyphenol oxidase and total phenol content were considerably increased. | ||
کلیدواژهها [English] | ||
PGPR, biocontrol, Pseudomonas fluorescens | ||
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