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اثر برخی پروبیوتیکهای گیاهی در کنترل زیستی عوامل زردی و پژمردگی فوزاریومی نخود | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
دوره 9، شماره 2، بهمن 1399، صفحه 143-159 اصل مقاله (862.53 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2021.324966.305 | ||
نویسندگان | ||
ناهید معرفزاده* 1؛ روح اله شریفی2؛ هادی خاطری1؛ سعید عباسی2 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
2دانشگاه رازی | ||
چکیده | ||
بیماری زردی و پژمردگی فوزاریومی نخود با عوامل قارچی Fusarium redolens (به اختصار: Fr) و Fusarium oxysporum f. sp. ciceris (به اختصار: Foc)، از بیماریهای مهم این محصول در ایران است. در این پژوهش اثر برخی سویههای پروبیوتیک باکتریایی و قارچی دریافتی از منابع مختلف که متعلق به جنسهای Alcaligenes، Bacillus، Delftia، Pseudomonas، Stenotrophomonas و Trichoderma بودند، در مهار بیمارگرهای مذکور در آزمایشگاه و گلخانه و تأثیرشان بر شاخصهای رشدی نخود در حضور بیمارگرها بررسی شد. ترکیبات فرّار کلیه پروبیوتیکها از رشد میسلیوم بیمارگرها جلوگیری نمودند. در آزمون کشت متقابل نیز همه پروبیوتیکها بر رشد Foc اثر بازدارنده داشتند، اما فقط اثر Bacillus pumilus INR7 و Trichoderma harzianum T33 در مهار رشد Fr معنیدار بود. در گلخانه، Delftia tsuruhatensis PIIR و1624 Alcaligenes faecalis به ترتیب با کاهش 38٫۲ و 23٫58 درصدی زردی و 35٫2 درصدی نکروز ریشه ناشی از Fr و افزایش فاکتورهای رشدی، و در مقابل، T. harzianum T33 و Pseudomonas putida RUP1 به ترتیب با کاهش 50 و ۶۶/۳۷ درصدی زردی و ۷۵/۴۴ و ۸۴/۳۷ درصدی نکروز آوندی ناشی از Foc و بهبود رشد نخود، جزو بهترین تیمارها بودند؛ در مجموع، پروبیوتیکهای مورد استفاده، پتانسیل تحریک رشد گیاه و بیوکنترل عوامل زردی و پژمردگی فوزاریومی نخود را داشتند. | ||
کلیدواژهها | ||
باکتریهای محرک رشد گیاه؛ بیماری خاکزاد؛ فاکتورهای رشدی؛ ترکیبات فرّار؛ کنترل زیستی | ||
عنوان مقاله [English] | ||
The effect of some plant probiotics in the biocontrol of the causal agents of chickpea Fusarium yellowing and wilting | ||
نویسندگان [English] | ||
Nahid Moarrefzadeh1؛ Rouhallah Sharifi2؛ Hadi Khateri1؛ Saeed Abbasi2 | ||
1Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran | ||
2Razi Univ | ||
چکیده [English] | ||
Chickpea Fusarium yellowing and wilting disease, caused by Fusarium redolens (Fr) and Fusarium oxysporum f.sp. ciceris (Foc) is one of the most common diseases of this plant in Iran. In this study, the effect of bacterial and fungal probiotic strains received from different sources belonging to Alcaligenes, Bacillus, Delftia, Pseudomonas, Stenotrophomonas and Trichoderma genera was investigated on mycelial growth of the plant pathogens in the laboratory and chickpea growth and disease indices in the greenhouse. The volatile compounds of all probiotics prevented the growth of pathogens mycelium. In dual-culture test, all probiotics had an inhibitory effect on Foc growth, but only the effect of Bacillus pumilus INR7 and Trichoderma harzianum T33 on Fr growth inhibition was significant. In the greenhouse, Delftia tsuruhatensis PIIR and Alcaligenes faecalis 1624 were among the best treatments in reducing Fr disease indices and increasing growth factors. In contrast, T. harzianum T33 and Pseudomonas putida RUP1 were the best strains in decreasing disease indices and improving plant growth from Foc infected plants. Overall, the probiotics had the potential for promoting plant growth and biocontrol of these pathogens and after the necessary studies, can be recommended in integrated management of Fusarium disease of chickpeas. | ||
کلیدواژهها [English] | ||
Plant growth promoting bacteria, Soil-borne disease, Growth factors, Volatile compounds, Biological control | ||
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