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بررسی کارایی گیاهبهسازی خاک آلوده به نفت خام با مایهزنی خاک با براکی‌باکتریوم موریس و سودوموناس پوتیدا | ||
| تحقیقات آب و خاک ایران | ||
| دوره 53، شماره 1، فروردین 1401، صفحه 171-187 اصل مقاله (1.61 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2022.335562.669163 | ||
| نویسندگان | ||
| هادی کوهکن* 1؛ محمد صدیق مرتضوی1؛ احمد گلچین2؛ غلامعلی اکبرزاده چماچایی1؛ فرشته سراجی1؛ محسن گذری1 | ||
| 1پژوهشکده اکولوژی خلیج فارس و دریای عمان، موسسه تحقیقات علوم شیلاتی کشور ،سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرعباس، ایران. | ||
| 2گروه علوم خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران. | ||
| چکیده | ||
| فرآوردههای نفتی از پرکاربردترین و گرانبهاترین مواد شیمیایی در دنیای مدرن به شمار میآیند ولی آلودگیهای وابسته به استخراج و حمل نفت خام به یک دشواری جهانی برای محیطزیست تبدیل شده است. در این آزمایش کارایی گیاهبهسازی، زیست پالایی و گیاهبهسازی زیستافزونی شده در حذف نفت خام از خاک بررسی شد. برای این کار، یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. فاکتورها شامل سه سطح آلودگی خاک به نفت (صفر، 4 و 8 درصد وزنی)، چهار تیمار گیاهی (بدون گیاه، برموداگراس (Cynodon dactylon)، سورگوم (bicolor Sorghum) و جو (Hordeum vulgare)) و سه تیمار باکتری (بدون باکتری، باکتری بومی (Brachybacterium muris) و باکتری غیربومی (سودوموناس پوتیدا)) بودند. نتایج نشان داد که درصد حذف نفت خام با کشت گیاه، مایهزنی خاک با باکتری و کاربرد هم‌زمان گیاه و باکتری به گونه چشمگیری در برابر شاهد افزایش یافت. کشت گیاه در برابر مایهزنی باکتری در کاهش غلظت مواد نفتی کاراتر بود و کارایی باکتریها با کشت گیاه به گونه چشمگیر افزایش یافت. در هر سطح از آلودگی، بیشترین درصد حذف نفت با کاربرد هم‌زمان سورگوم و باکتری Brachybacterium muris دیده شد. در همه تیمار گیاهی، بالاترین درصد حذف نفت در سطح 4 درصد آلودگی نفتی و مایهزنی Brachybacterium muris اندازهگیری شد. آلودگی نفتی وزن خشک و غلظت کلروفیل برگ را به گونه چشمگیری کاهش داد ولی کاربرد باکتریها (به ویژه باکتری بومی) اثرات منفی آلودگی نفتی بر گیاهان را به گونه چشمگیری در برابر تیمارهای بدون مایهزنی کاهش داد. آلودگی نفتی غلظت پرولین در برگ گیاهان را افزایش داد و با کاربرد باکتری بومی غلظت پرولین کاهش یافت. استقرار گیاه به همراه ریز جانداران میتواند به‌عنوان جز کلیدی استراتژی حذف هیدروکربنهای نفتی در نظر گرفته شود. پس این گونه باکتریها و گیاهان را میتوان برای زیست پالایی خاکهای آلوده به نفت خام بکار برد. | ||
| کلیدواژه‌ها | ||
| آلودگی نفتی؛ براکی باکتریوم موریس؛ برموداگراس؛ سورگوم؛ گیاهبهسازی زیستافزونی شده | ||
| عنوان مقاله [English] | ||
| The Study of the Phytoremediation Efficiency of Crude Oil Contaminated Soil by Inoculating the Soil with Brachybacterium muris and Pseudomonas putida | ||
| نویسندگان [English] | ||
| Hadi Koohkan1؛ Mohammad Sedigh Mortazavi1؛ Ahmad Golchin2؛ Gholamali Akbarzadeh- Chomachaei1؛ Fereshteh Saraji1؛ Mohsen Gozari1 | ||
| 1Persian Gulf and Oman Sea Ecological Research Institute, Iranian fisheries science Research Institute, Agricultural Education and Extension Research Organization, Bandar Abbas, Hormozgan, Iran | ||
| 2Soil Science Department, Faculty of Agriculture, Zanjan University of Zanjan, Zanjan, Iran. | ||
| چکیده [English] | ||
| Petroleum products are considered to be the most widely used and expensive chemicals in the modern world, but pollution from the extraction and transportation of crude oil has become a global environmental problem. In this experiment, the efficiency of phytoremediation, bioremediation and bio-enhanced phytoremediation in removing crude oil from soil was investigated. For this purpose, a factorial experiment was performed in a completely randomized design with three replications. Factors include three levels of soil oil pollution (zero, 4 and 8% by weight), four plant treatments (without plants, bermudagras (Cynodon dactylon), sorghum (Bicolor Sorghum) and barley (Hordeum vulgare)) and three bacterial treatments (without bacteria). Were native bacteria (Brachybacterium muris) and non-native bacteria (Pseudomonas putida). The results showed that the percentage of crude oil removal by plant cultivation, soil inoculation with bacteria and combined application of plant and bacteria increased significantly compared to the control. Plant culture was more effective than bacterial inoculation in reducing the concentration of petroleum products and the efficiency of bacteria increased significantly with plant culture. At each level of contamination, the highest percentage of oil removal was observed with the combined application of sorghum and Brachybacterium muris. In all plant treatments, the highest oil removal percentage was measured at 4% oil pollution and Brachybacterium muris inoculation. Oil pollution significantly reduced leaf dry weight and chlorophyll concentration, but the use of bacteria (especially native bacteria) significantly reduced the negative effects of oil pollution on plants compared to non-inoculation treatments. Oil pollution increased the proline concentration in the leaves of plants and decreased the proline concentration with the use of native bacteria. Establishment of plant with microorganisms can be considered as a key component of the strategy to remove hydrocarbons. Consequently, these bacterial and plant species can be used for the biodegradation of soils contaminated with crude oil. | ||
| کلیدواژه‌ها [English] | ||
| petroleum pollution, Brachybacterium muris, bermudagras, sorghum, bioaugmented phytoremediation | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 335 تعداد دریافت فایل اصل مقاله: 285 |
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