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کاهش زیستی کروم شش ظرفیتی در خاک آلوده با استفاده از باکتری غیرمتحرک شده روی بیوچار جلبک | ||
تحقیقات آب و خاک ایران | ||
دوره 53، شماره 7، مهر 1401، صفحه 1467-1479 اصل مقاله (1.38 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijswr.2022.342878.669265 | ||
نویسندگان | ||
مریم خلیلی راد* ؛ نازنین اسماعیلی؛ محمدباقر فرهنگی؛ نسرین قربان زاده | ||
ﮔﺮوه ﻋﻠﻮم و ﻣﻬﻨﺪﺳﯽ ﺧﺎک، داﻧﺸﮑﺪه علوم ﮐﺸﺎورزی، داﻧﺸﮕﺎه ﮔﯿﻼن، رﺷﺖ، اﯾﺮان | ||
چکیده | ||
هدف این پژوهش حذف کروم شش ظرفیتی (VI) از یک خاک آلوده از طریق کاهش آن به کروم سه ظرفیتی (III) توسط باکتری Shewanella sp.. غیرمتحرک شده بر روی بیوچار جلبک و بررسی انباشتگی آن در گیاه جو بود. خاک لوم شنی با mg kg-1 50 کروم (VI) آلوده و به مدت دو هفته انکوباسیون شد. سپس آزمایش گلدانی کشت جو در پاییز 1400 در گلخانه تحقیقاتی دانشگاه گیلان در قالب طرح کاملاً تصادفی در سه تکرار در خاک آلوده انجام شد. تیمارها شامل باکتری Shewanella sp. (S)، بیوچار جلبک (B)، بیوچار جلبک + باکتری Shewanella sp. (BS) و باکتری Shewanella sp.. غیرمتحرک شده بر روی بیوچار جلبک (IB) بودند. خاک آلوده به کروم (VI) و غیرآلوده نیز به ترتیب کنترل مثبت (C+) و منفی (C-) بودند. گیاه پس از 30 روز برداشت و وزن خشک ریشه و اندام هوایی آن اندازهگیری شد. مقدار کروم کل و کروم (VI) در ریشه، اندام هوایی گیاه و خاک اندازهگیری شد و مقدار کروم (III) از تفاضل مقدار کروم کل و کروم (VI) به دست آمد. نسبت رشد، فاکتور انباشت کروم (VI) در ریشه و اندام هوایی و فاکتور انتقال آن در گیاه محاسبه شد. بیشترین و کمترین مقدار کروم (III) در ریشه، اندام هوایی و خاک به ترتیب در تیمارهای IB و C+ به دست آمد. در تیمارهای آلوده به کروم (VI) بیشترین و کمترین مقدار وزن خشک ریشه و اندام هوایی به ترتیب در تیمارهای IB و C+ مشاهده شد. در تیمار BS کاهش کروم (VI) در خاک نسبت به دو تیمار S و B بیشتر بود. کمترین و بیشترین نسبت رشد گیاه به ترتیب در تیمار C+ (8/23 درصد) و IB (60 درصد) مشاهده شد. تیمارهای BS و IB سبب کاهش بیشتر فاکتور انتقال شد. بنابراین غیرمتحرک کردن باکتری بر روی بیوچار میتواند در پالایش خاکهای آلوده به کروم موثر باشد. | ||
کلیدواژهها | ||
زیستپالایی؛ Shewanella sp؛ فاکتور انتقال؛ فاکتور انباشت؛ کروم (III) | ||
عنوان مقاله [English] | ||
Chromium (VI) Bioreduction in Contaminated Soil using Bacterium Immobilized on Algae Biochar | ||
نویسندگان [English] | ||
Maryam Khalilirad؛ Nazanin Esmaeali؛ Mohammad Bagher Farhangi؛ Nasrin Ghorbanzadeh | ||
Department of Soil Science, Faculty of Agriculture, University of Guilan, Rasht, Iran | ||
چکیده [English] | ||
The objective of this study was to eliminate chromium(VI) from a contaminated soil through reducing it to chromium(III) by immobilized Shewanella sp. bacteria on algae biochar and to investigate its accumulation in barley. A sandy loam soil was contaminated with 50 mg kg-1 Cr(VI) and incubated for two weeks. Then, the barley pot experiment in the contaminated soil was performed in the autumn of 1400 in the research greenhouse of University of Guilan in a completely randomized design with three replications. Treatments included Shewanella sp. (S), algae biochar (B), algae biochar + Shewanella sp. (BS), and immobilized Shewanella sp. bacteria on algae biochar (IB). Soil contaminated with Cr(VI) and non-contaminated soils were also included as positive (C+) and negative (C-) controls, respectively. After 30 days, the plant was harvested and dry weight of root and shoot were measured. Total chromium and Cr(VI) contents in the root and shoot of plant and also in the soil were measured. The Cr(III) content was calculated from the difference between the total chromium and the Cr(VI) contents. Growth ratio, Cr(VI) accumulation factor in roots and shoots and the transfer factor in plants were calculated. The highest and lowest amounts of Cr(III) in roots, shoots and soil were obtained in IB and C+ treatments, respectively. In Cr(VI) contaminated treatments, the highest and the lowest values of root and shoot dry weight were obtained in IB and C+ treatments, respectively. Cr(VI) reduction in soil in BS treatment was more than S and B treatments. The lowest and the highest plant growth ratios were obtained in C+ (23.8%) and IB (60%) treatments, respectively. The BS and IB treatments further reduced the transfer factor. Therefore, immobilized bacteria on biochar can be effective in remediation of chromium contaminated soils. | ||
کلیدواژهها [English] | ||
Accumulation factor, Bioremediation, Chromium (III), Shewanella sp, Transfer factor | ||
مراجع | ||
Alloway, B. J. (2013). Heavy Metals in Soils: Trace Metals and Metalloids in Soils and their Bioavailability. 3 ed. Springer, Netherlands. Ancona, V., Campanale, C., Tumolo, M., De Paola, D., Ardito, C., Volpe, A. and Uricchio, V. F. (2020). Enhancement of chromium (VI) reduction in microcosms amended with lactate or yeast extract: A laboratory-scale study. International Journal of Environmental Research and Public Health, 17, 704. Aparicio, J. D., Lacalle, R. G., Artetxe, U., Urionabarrenetxea, E., Becerril, J. M. and Polti, M. A. (2021). Successful remediation of soils with mixed contamination of chromium and lindane: Integration of biological and physicochemical strategies. Environmental Research, 194, 110666. Ayele, A. and Godeto, Y. G. (2021). Bioremediation of chromium by microorganisms and its mechanisms related to functional groups. Journal of Chemistry, 7694157. Banerjee, A., Nayak, D., Chakrabortty, D. and Lahiri, S. (2008). Uptake studies of environmentally hazardous Cr in Mung beans. Environmental Pollution, 151(2), 423-7. Bashir, M. S., Wang, X., Naveed, M., Mustafa, A., Ashraf, S., Samreen, T., Nadeem, S.M. and Jamil, M. (2021). Biochar mediated-alleviation of chromium stress and growth improvement of different maize cultivars in tannery polluted soils. International Journal of Environmental Research and Public Health, 18, 4461. Bremner, J. M. and Mulvaney, C. S. (1982). Nitrogen-total. In: A. L. Page (Ed.), Methods of Soil Analysis (Part 2). (pp. 595-624). American Society of Agronomy, Madison: WI. Buendia-Gonzalez, L., Orozco-Villafuerte, J., Cruz-Sosa, F., Barrera-Diaz, C. E. and Vernon-Carter, E. J. (2010). Prosopis laevigata a potential chromium(VI) and cadmium(II) hyper accumulator desert plant. Bioresource Technology, 101, 5862-5867. Caldelas, C., Bort, J. and Febrero, A. (2011). Ultrastructure and subcellular distribution of Cr in Iris pseudacorus L. using TEM and X-ray microanalysis. Cell Biology and Toxicology, 28(1), 57–68. Chatterjee, J. and Chatterjee, C. (2000). Phytotoxicity of cobalt, chromium and copper in cauliflower. Environmental Pollution, 109, 69–74. Chen, W.H., Lin, B.J., Huang, M.Y. and Chang, J.S., (2015). Thermochemical conversion of Chen, Y., Wu, H., Sun, P., Liu, J., Qiao, S., Zhang, D. and Zhang, Z. (2021). Remediation of chromium-contaminated soil based on Bacillus cereus WHX-1 immobilized on biochar: Cr(VI) transformation and functional microbial enrichment. Frontiers in Microbiology, 12, 641913. Choppala, G. K., Bolan, N. S., Megharaj, M., Chen, Z. and Naidu, R. (2012). The influence of biochar and black carbon on reduction and bioavailability of chromate in soils. Journal of Environmental Quality, 41, 1175–1184. Choudhary, B., Paul, D., Singh, A., and Gupta, T. (2017). Removal of hexavalent chromium upon interaction with biochar under acidic conditions: mechanistic insights and application. Environmental Science and Pollution Research, 24(20), 16786-16797. Clesceri, L. S., Greenberg, A. E. and Trussell, R.R. (1996). Standard methods for the examination of water and wastewater. Washington DC: APHA, AWWA and WPCF. Focardi, S., Pepi, M. and Focardi, E. S. (2013). Microbial reduction of hexavalent chromium as a mechanism of detoxification and possible bioremediation applications. Biodegradation -Life of Science, 321-347. Gee, G. W. and Bauder, J. W. (1986). Particle size analysis. In: A. Klute (Ed.), Method of Soil Analysis (Part 1). (pp. 383-411). American Society of Agronomy, Madison: WI. Hale, L., Luth, M., Kenney, R. and Crowley, D. (2014). Evaluation of pinewood biochar as a carrier of bacterial strain Enterobacter cloacae UW5 for soil inoculation. Applied Soil Ecology, 84, 192–199. Huang, J. H, Voegelin, A, Pombo, S. A., Lazzaro, A, Zeyer, J. and Kretzschmar, R. (2011). Influence of arsenate adsorption to ferrihydrite, goethite, and boehmite on the kinetics of arsenate reduction by Shewanella putrefaciens strain CN-32. Environmental Science & Technology, 45, 7701–7709. Imran, M., Khan, Z. U. H., Iqbal, M. M., Iqbal, J., Shah, N. S., Munawar, S., Ali, S., Murtaza, B., Naeem, M. A. and Rizwan, M. (2020). Effect of biochar modified with magnetite nanoparticles and HNO3 for efficient removal of Cr(VI) from contaminated water: A batch and column scale study. Environmental Pollution, 261, 114231. Jiang, Y., Yang, F., Dai, M., Ali, I., Shen, X., Hou, X., Alhewairini, S. S., Peng, C. and Naz, I. (2022). Application of microbial immobilization technology for remediation of Cr(VI) contamination: A review. Chemosphere, 286, 131721. Karthik, C., Elangovan., N., Kumara, T. S., Govindharaju, S., Barathi, S., Oves, M. and Arulselvi, P. I. (2017). Characterization of multifarious plant growth promoting traits of rhizobacterial strain AR6 under Chromium (VI) stress. Microbiological Research, 204, 65-71. Khalid, S., Shahid, M., Niazi, N. K., Murtaza, B., Bibi, I. and Dumat, C. (2017). A comparison of technologies for remediation of heavy metal contaminated soils. Journal of Geochemical Exploration, 182, 247–268. Knudsen, D., Peterson, G. A. and Pratt, P. F. (1982). Lithium, Sodium and potassium. In: A. L. Page et al. (Ed.) Methods of Soil Analysis (Part 2). (pp. 225-246). American Society of Agronomy, Madison: WI. Li, H., Dong, X., da Silva, E. B., de Oliveira, L. M., Chen, Y. and Ma, L.Q. (2017). Mechanisms of metal sorption by biochars: Biochar characteristics and modifications. Chemosphere, 178, 466-478. Liu, J., Pan, D., Wu, X., Chen, H., Cao, H., Li, Q. X. and Hua, R. (2018). Enhanced degradation of prometryn and other s-triazine herbicides in pure cultures and wastewater by polyvinyl alcohol-sodium alginate immobilized Leucobacter sp. JW-1. Science of Total Environment, 615, 78–86. Lu, H., Zhang, Y. Y., Huang, X., Wang, S. and Qiu, R. (2012). Relative distribution of Pb2+ sorption mechanisms by sludge-derived biochar. Water Resources, 46, 854–862. Malekotti M. J. and Ghayibi M. N. (2000). Determining the critical level of effective nutrients in soil, plants and fruits in order to increase the quality and quantity performance of strategic products of the country. second edition. Agricultural Education Publication. (in Persian). Mandal, S., Sarkar, B., Bolan, N., Ok, Y. S. and Naidu, R. (2016). Enhancement of chromate reduction in soils by surface modified biochar. Journal of Environmental Management, 186, 277-284. Olsen, S. R., Cole, C. V., Watanabe, F. S. and Dean, L. A. (1954). Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. USDA Circular, (939): 1-19. Department of Agriculture: Washington D. C. Page, A. L., Miller, R. H. and Keeney, D. R. (1982). Methods of Soil Analysis (Part 2). American Society of Agronomy, Madison: WI Patra, D. K., Pradhan, C. and Patra, H. K. (2019). Chromium bioaccumulation, oxidative stress metabolism and oil content in lemon grass Cymbopogon flexuosus (Nees ex Steud.) W. Watson grown in chromium rich over burden soil of Sukinda chromite mine, India. Chemosphere, 218, 1082-1088. Polti, M. A., García, R. O., Amoroso, M. J. and Abate, C. M. (2009). Bioremediation of chromium (VI) contaminated soil by Streptomyces sp. MC1. Journal of Basic Microbiology, 49, 285–292. Qayyum, S., Khan, I., Meng, K., Zhao, Y. and Peng, C. (2020). A review on remediation technologies for heavy metals contaminated soil. Central Asian Journal of Environmental Science and Technology Innovation, 1(1), 21–29. Rajapaksha, A. U., Alam, Md. S., Chen, N. Alessi, D. S., Igalavithana, A. D., Tsang, D. C.W. and Oka, Y. S. (2018). Removal of hexavalent chromium in aqueous solutions using biochar: Chemical and spectroscopic investigations. Science of the Total Environment, 625, 1567–1573. Rajkovich, S., Enders, A., Hanley, K., Hyland, C., Zimmerman, A. R. and Lehmann, J. (2012). Corn growth and nitrogen nutrition after additions of biochars with varying properties to a temperate soil. Biology and Fertility of Soils, 48, 271–284. Schikora, A. and Schmidt, W. (2001). Iron stress-induced changes in root epidermal cell fate are regulated independently from physiological responses to low iron availability. Plant Physiology, 125, 1679–1687. Shanker, A. K., Cervantes, C., Loza-Tavera, H. and Avudainayagam, S. (2005). Chromium toxicity in plants. Environment International, 31, 739–753. Sharma, S., Tiwari, S., Hasan, A., Saxena, V. and Pandey, L. M. (2018). Recent advances in conventional and contemporary methods for remediation of heavy metal-contaminated soils. Biotechnology, 8(4), 216. Shi, Y., Shan, R., Lu, L., Yuan, H., Jiang, H., Zhang, Y. and Chen, Y. (2020). High-efficiency removal of Cr(VI) by modified biochar derived from glue residue. Journal of Cleaner Production, 254, 119935. Sidhu, G. P. S., Singh, H. P., Batish, D. R. and Kohli, R. K. (2017). Tolerance and hyperaccumulation of cadmium by a wild, unpalatable herb Coronopus didymus (L.) Sm. (Brassicaceae). Ecotoxicology and Environmental Safety, 135, 209–215. Singh, P., Itankar, N. and Patil, Y. (2020). Biomanagement of hexavalent chromium: current trends and promising perspectives. Journal of Environmental Management, 279 (1), 111547. Srivastava, D., Tiwari, M., Dutta, P., Singh, P, Chawda, K., Kumari, M. and Chakrabarty, D. (2021). Chromium stress in plants: Toxicity, Tolerance and Phytoremediation. Sustainability, 13, 4629. Sun, D. Q., Lan, Y., Xu, E. G., Meng, J. and Chen, W. F. (2016). Biochar as a novel niche for culturing microbial communities in composting. Waste Management, 54, 93–100. Sundaramoorthy, P., Chidambaram, A., Ganesh, K. S., Unnikannan, P. and Baskaran, L. (2010). Chromium stress in paddy: (i) nutrient status of paddy under chromium stress; (ii) phytoremediation of chromium by aquatic and terrestrial weeds. Comptes Rendus Biologies, 333(8), 597-607. Walkley, A. and Black, I. A. (1934). An examination of digestion method for determining soil organic matter and a proposed modification of the chromic acid titration. Soil Science, 37, 29–38. Wang, C. and Cui, Y. (2019). Recognition of a new Cr(VI)-reducing strain and study of the potential capacity for reduction of Cr(VI) of the strain. BioMed Research International, 5135017. Xu, X. Y., Huang, H., Zhang, Y., Xu, Z. B. and Cao, X. D. (2019). Biochar as both electron donor and electron shuttle for the reduction transformation of Cr(VI) during its sorption. Environmental Pollution, 244, 423–430. Xu, X., Cao, X., Zhao, L., Wang, H., Yu, H. and Gao, B. (2013). Removal of Cu, Zn, and Cd from aqueous solutions by the dairy manure-derived biochar. Environmental Science and Pollution Research, 20, 358–368. Xu, Y., Liu, J. Q., Cai, W. S., Feng, J. Y., Lu, Z. J. and Wang, H. Z. (2020). Dynamic processes in conjunction with microbial response to disclose the biochar effect on pentachlorophenol degradation under both aerobic and anaerobic conditions. Journal of Hazardous Materials, 384, 121503. Yang, X. D., Wan, Y. S., Zheng, Y. L., He, F., Yu, Z. B., Huang, J., Wang, H. L., Ok, Y. S., Jiang, Y. S. and Gao, B. (2019). Surface functional groups of carbon-based adsorbents and their roles in the removal of heavy metals from aqueous solutions: A critical review. Chemical Engineering Journal, 366, 608-621. Yu, K. L., Lau, B. F., Show, P. L., Ong, H. C., Ling, T.C., Chen, W. H., Ng, N. P. and Chang, J. S. (2017). Recent developments on algal biochar production and characterization. Bioresource Technology, 246, 2–11. Zayed, A., Lytle, C. M., Qian, J. H. and Terry, N. (1998). Chromium accumulation, translocation and chemical speciation in vegetable crops. Planta, 206, 293-299. Zeng, F., Ali, S., Qiu, B., Wu, F. and Zhang, G. (2010). Effects of chromium stress on the subcellular distribution and chemical form of Ca, Mg, Fe, and Zn in two rice genotypes. Journal of Plant Nutrition and Soil Science, 173, 135–148. Zhang, X., Lv, L., Qin, Y., Xu, M., Jia, X. and Chen, Z. (2018). Removal of aqueous Cr(VI) by a magnetic biochar derived from Melia azedarach wood. Bioresource Technology, 256, 1−10. Zhao, Y. and Han, G. (1994). Rapid spectrophotometric determination of chromium(III). Talanta, 41(8), 1247-1250. Zheng, C., Yang, Z., Si, M., Zhu, F., Yang, W., Zhao, F. and Shi, Y. (2020). Application of biochars in the remediation of chromium contamination: Fabrication, mechanisms, and interfering species. Journal of Hazardous Materials, 407, 124376. | ||
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