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The Relative Frequency of Histomonas meleagridis Infection in Turkey Flocks in Some Provinces of Iran | ||
Iranian Journal of Veterinary Medicine | ||
مقاله 6، دوره 18، شماره 3، مهر 2024، صفحه 387-396 اصل مقاله (1.94 M) | ||
نوع مقاله: Original Articles | ||
شناسه دیجیتال (DOI): 10.32598/ijvm.18.3.1005384 | ||
نویسندگان | ||
Ali Salavati1؛ Seyed Mostafa Peighambari1؛ Azam Yazdani1؛ Hesameddin Akbarein2؛ Jamshid Razmyar* 1 | ||
1Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. | ||
2Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. | ||
چکیده | ||
Background: Histomoniasis is caused by the protozoan Histomonas meleagridis with an intermediate host of Heterakis gallinarum, which results in ulceration of the ceca walls, enlargement of the ceca by large casts, mesenteric inflammation, and liver necrosis. This disease is very important in Iran’s growing turkey breeding industry. Objectives: The present study aims to evaluate the relative frequency of H. meleagridis infection in different turkey flocks to draw a cross-sectional picture of H. meleagridis infection in Golestan, Mazandaran, Guilan, and Tehran provinces of Iran. Methods: This study is a cross-sectional survey of H. meleagridis infection during spring. Dropping samples were taken from backyard and commercial turkey flocks. After taking the fecal samples, they were investigated by Giemsa staining under a light microscope. A PCR test was performed to confirm the diagnosis of infection. Results: Out of 240 samples (from 19 flocks), 20 infected samples were detected by direct microscopic observation of H. meleagridis, and PCR confirmed 15 samples. Conclusion: The results of this study showed that the relative frequency of H. meleagridis infection was lower than in similar studies in other parts of the world. This finding may be due to Iran’s less widespread use of turkey production. Considering the growth of the turkey production industry in Iran over the last decade and its further production over the next few years, it is necessary to evaluate histomoniasis. | ||
کلیدواژهها | ||
Giemsa staining؛ Histomonas meleagridis؛ Histomoniasis؛ PCR؛ Turkey | ||
عنوان مقاله [English] | ||
فراوانی نسبی آلودگی به هیستوموناس مله اگریدیس در گلههای بوقلمون در چند استان ایران | ||
نویسندگان [English] | ||
علی صلواتی1؛ سید مصطفی پیغمبری1؛ اعظم یزدانی1؛ حسامالدین اکبرین2؛ جمشید رزم یار1 | ||
1گروه بیماریهای طیور، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران. | ||
2گروه بهداشت و کنترل کیفی مواد غذایی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران. | ||
چکیده [English] | ||
زمینه مطالعه: هیستومونیازیس توسط تک یاخته هیستوموناس ملهاگریدیس با میزبان واسط هتراکیس گالیناروم ایجاد میشود که به زخم شدن دیوارههای سکوم، بزرگ شدن آن توسط کستهای بزرگ، التهاب مزانتریک و نکروز کبد منجر میشود. این بیماری در صنعت روبهرشد پرورش بوقلمون در ایران بسیار حائز اهمیت است. هدف: مطالعه حاضر باهدف بررسی فراوانی نسبی عفونت هیستوموناس مله اگریدیس در گلههای مختلف بوقلمون به منظور ترسیم تصویر مقطعی از این عفونت هیستوموناس در استانهای گلستان، مازندران، گیلان و تهران در ایران انجام شد. روش کار: این مطالعه یک بررسی مقطعی از عفونت هیستوموناس مله اگریدیس است. نمونهبرداری از گلههای بوقلمون بومی و صنعتی صورت گرفت. پس از گرفتن نمونه مدفوع، مشاهده انگل در رنگآمیزی گیمسا در زیر میکروسکوپ نوری مورد بررسی قرار گرفت. برای تأیید تشخیص عفونت، آزمایش PCR انجام شد. نتایج: از 240 نمونه، 20 نمونه با مشاهده میکروسکوپی مستقیم هیستوموناس مله اگریدیس و 15 نمونه با روش PCR تأیید شد. نتیجهگیری نهایی: نتایج این مطالعه نشان داد فراوانی عفونت هیستوموناس مله اگریدیس نسبت به مطالعات مشابه در سایر نقاط جهان کمتر است. این مسئله ممکن است بهدلیل حجم پرورش کمتر بوقلمون در ایران باشد. باتوجهبه اینکه صنعت تولید بوقلمون در ایران در دهه اخیر رشد چشمگیری داشته است و با پیشبینی روند روبهرشد این صنعت در سالهای آینده، اهمیت مطالعه هیستومونیازیس بیشازپیش احساس میشود. | ||
کلیدواژهها [English] | ||
رنگآمیزی گیمسا, هیستوموناس مله اگریدیس, هیستومونیازیس, PCR, بوقلمون | ||
اصل مقاله | ||
Introduction
Of the 19 flocks surveyed, 9 were commercial, and 10 were backyard flocks. A total of 5 flocks (1 commercial and 4 backyards) were positive for H. meleagridis infection (Figure 1).
From 240 samples, 181 samples from commercial flocks and 59 samples from backyard flocks were collected. One sample (0.55% with 95% CI, -10.68%, 79.79%) and 14 samples (23.73% with 95% CI) collected from commercial and backyard flocks, respectively, were positive for H. meleagridis infection (Table 3). Using the Fisher exact test, a statistically significant difference was observed between positive cases (based on Giemsa staining and PCR test) and production type (P<0.001).
According to Figure 2, row indicates 100-2000 kbp ladder, row PC indicates positive control, row NC indicates negative control, and rows 1 to 6 are samples. Samples 1 and 4 are positive, and the rest are negative.
In terms of flock size, commercial flocks were divided into two groups: Low numbers (below 2000 birds per commercial unit) and high numbers (above 2000 birds per commercial unit) (Table 5). Using the Fisher exact test, a statistically significant difference was observed between the positive cases (based on Giemsa staining) and the number of birds kept in industrial units (P=0.039), but using the same test, no statistically significant difference between the positive cases (based on PCR test) and the number of birds kept in industrial units was observed (P=0.199).
Discussion
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