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بررسی اثر غلظت‌های متفاوت کلرید سرب بر بیان ژن‌های مؤثر در فاکتور های ایمنی ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) | ||
| شیلات | ||
| دوره 76، شماره 4، دی 1402، صفحه 655-666 اصل مقاله (1.32 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2024.362092.1395 | ||
| نویسندگان | ||
| بهناز جعفری1؛ امیررضا عابد علم دوست* 2؛ حمید فرحمند3 | ||
| 1دانشجوی کارشناسی ارشد گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
| 2استادیار گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
| 3استاد گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
| چکیده | ||
| سرب یکی از فلزات سنگین با اثر سمی است که در آب‌های داخلی و مخازن پرورشی یافت می‌شود و می‌تواند باعث ایجاد عفونت و بیماری‌هایی در آبزیان و انسان گردد. هدف از انجام این مطالعه بررسی اثر فلز سرب در بچه ماهی نورس قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) و اثر آن بر بیان ژن‌های مرتبط با ایمنی (MHC I، IL-6، TNFα) و ژن پروتئین شوک حرارتی (HSP70) است. پس از تکثیر و پرورش ماهیان در آب فاقد سرب، بچه ماهی های نورس نیم گرمی در سه تیمار مختلف سرب کلرید (PbCl2) با غلطت‌های (0/25 و 0/5 و ۱) میکرو مول بر لیتر و گروه شاهد در سه تکرار (35 قطعه بچه ماهی در هر تکرار) به‌مدت 96 ساعت در معرض قرار گرفتند. سپس نمونه‌های تصادفی در ازت مایع منجمد شد و برای آزمایش بیان ژن استفاده شدند. آزمایش بیان ژن با روش Real-time PCR و با آغازگر اختصاصی انجام شد. نتایج تحلیل داده‌ها و منحنی‌های تکثیر و ذوب نشان داد که ژن پروتئین شوک حرارتی HSP70 در دو غلظت 0/25 و یک میکرومول بر لیتر، تغییرات معنی‌داری دارد (0.05>P) و منحنی U شکل برعکس نشان داد که احتمالاً این امر به‌دلیل اثر هورموزیس سرب است. بیان ژن‌های اینترلوکین شش و MHC I در تیمار با غلظت 1 افزایش معنی‌دار داشت (0.05>P)، همچنین ژن TNFα نیز بر خلاف HSP70 افزایش داشت که نشان‌دهنده منحنی U شکل و احتمالاً اثر هورموزیس عادی است. نتایج حاصل از این مطالعه نشان داد که سرب حتی در غلظت‌های در حدود آب آشامیدنی می‌توانند باعث تغییر بیان ژن‌های ناشی از تنش و شاخصهای مرتبط با ایمنی گردد و اثرات آن صرفاً به مسمومیت ختم نخواهد شد. | ||
| کلیدواژه‌ها | ||
| قزل‌آلای رنگین‌کمان؛ سرب؛ بیان ژن؛ اثر هورموزیس؛ شاخصهای ایمنی | ||
| عنوان مقاله [English] | ||
| Investigating the effect of different concentrations of lead chloride on the expression of genes effective in the immune factors of rainbow trout (Oncorhynchus mykiss) | ||
| نویسندگان [English] | ||
| Behnaz Jafari1؛ Amirreza Abed-Elmdoust2؛ Hamid Farahmand3 | ||
| 1MSc. student, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Assistant professor,, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Professor,, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| چکیده [English] | ||
| Lead is one of the heavy metals with a toxic effect found in inland waters and breeding tanks and can cause infections and diseases in aquatic animals and humans. The purpose of this study is to investigate the effect of lead metal in juvenile rainbow trout (Oncorhynchus mykiss) and its effect on the expression of immune-related genes (MHC I, IL-6, TNFα) and heat shock protein gene (HSP70). After reproduction and rearing of fishes in lead-free water, the fry (0.5 gr) was allocated to three different treatments of lead chloride (PbCl2) with concentrations (0.25, 0.5, and 1) micromole/liter and a control group in three replicates (35 fry per replicate) for 96 hours. Then random samples were frozen in liquid nitrogen and used for gene expression testing by Real-time PCR. The results revealed that the HSP70 heat shock protein gene had significant changes (P<0.05) in two concentrations of 0.25 and 1 micromole/liter and the reverse U-shaped curve showed that this is probably due to the effect of lead hormesis. The expression of interleukin 6 and MHC I genes had a significant increase (P<0.05) in concentration, and the TNF-α gene also increased, unlike HSP70, which indicated a U-shaped curve and probably the effect of normal hormesis. The results of this study showed that lead, even in concentrations close to drinking water, can change the expression of genes caused by stress and factors related to immunity, and its effects do not end only with poisoning. | ||
| کلیدواژه‌ها [English] | ||
| Rainbow trout, Lead, Gene expression, Hormosis effect, Immune factors | ||
| مراجع | ||
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Abedi, Z., Khalesi, M.K., Kohestan, E.S., Rahmani, H., 2012. Comparison of lethal concentrations (LC50-96 h) of CdCl2, CrCl3, and Pb (NO3) 2 in common carp (Cyprinus carpio) and Sutchi Catfish (Pangasius hypophthalmus). Iranian Journal of Toxicology 672-680. Abós, B., Wang, T., Castro, R., Granja, A. G., Leal, E., Havixbeck, J., Tafalla, C., 2016. Distinct differentiation programs triggered by IL-6 and LPS in teleost IgM+ B cells in the absence of germinal centers. Scientific Reports 6(1), 30004. DOI: 10.1038/srep30004 Braunbeck, T., Burkhardt-Holm, P., Görge, G., Nagel, R., Negele, R.D., Storch, V.,1992. Rainbow trout and zebrafish, two models for continuous toxicity tests: relative sensitivity, species and organ specificity in cytopathologic reaction of liver and intestines to atrazine. Schriftenreihe des Vereins fur Wasser-, Boden-und Lufthygiene 89, 109-145. Das, S., Mandal, S., Dhara, H., & Chatterjee, P., 2022. Dietary amelioration of lead toxicity in fish-A review. Indian Journal of Animal Health 61(2), 173-189. DOI: 10.36062/ijah.2022.spl.03422 Fischer, U., Dijkstra, J. M., Köllner, B., Kiryu, I., Koppang, E. O., Hordvik, I., Ototake, M., 2005. The ontogeny of MHC class I expression in rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology 18(1), 49-60. DOI: 10.1016/j.fsi.2004.05.006 Giri, S.S., Kim, M.J., Kim, S.G., Kim, S.W., Kang, J.W., Kwon, J., Park, S.C., 2021. Role of dietary curcumin against waterborne lead toxicity in common carp Cyprinus carpio. Ecotoxicology and Environmental Safety 219, 112318. DOI: 10.1016/j.ecoenv.2021.112318 Ghosh, S., Debnath, S., Rout, S.K., 2021. Effects of lead (Pb) toxicity on fish: a review. Journal of Experimental Zoology India 24. Holladay, S., Ehrich, M., Gogal Jr, R., 2005. Commentary on hormetic dose-response relationships in immunology: Occurrence, quantitative features of the dose response, mechanistic foundations, and clinical implications. Critical Reviews in Toxicology 35(2/3), 299. DOI: 10.1080/10408440590917080 Huseen, H.M., Mohammed, A.J., 2019. Heavy metals causing toxicity in fishes. Paper presented at the Journal of Physics: Conference Series. Jiang, Y., Möller, G., 1995. In vitro effects of HgCl2 on murine lymphocytes. I. Preferable activation of CD4+ T cells in a responder strain. Journal of Immunology (Baltimore, Md.: 1950) 154(7), 3138-3146. DOI: 10.4049/jimmunol.154.7.3138 Jing, H., Zhang, Q., Li, S., Gao, X.-j., 2020. Pb exposure triggers MAPK-dependent inflammation by activating oxidative stress and miRNA-155 expression in carp head kidney. Fish & Shellfish Immunology 106, 219-227. DOI: 10.1016/j.fsi.2020.08.015 Karatsoreos, I.N., McEwen, B.S., 2013. Annual research review: The neurobiology and physiology of resilience and adaptation across the life course. Journal of Child Psychology and Psychiatry 54(4), 337-347. DOI: 10.1111/jcpp.12054 Krocova, Z., Macela, A., Kroca, M., Hernychova, L., 2000. The immunomodulatory effect (s) of lead and cadmium on the cells of immune system in vitro. Toxicology in Vitro 14(1), 33-40. DOI: 10.1016/S0887-2333(99)00089-2 Kubysheva, N., Boldina, M., Eliseeva, T., Soodaeva, S., Klimanov, I., Khaletskaya, A., Ramírez-Salinas, M.A., 2020. Relationship of serum levels of IL-17, IL-18, TNF-α, and lung function parameters in patients with COPD, asthma-COPD overlap, and bronchial asthma. Mediators of Inflammation. 2020, 4652898. DOI: 10.1155/2020/4652898 Lee, J.-W., Choi, H., Hwang, U.-K., Kang, J.-C., Kang, Y. J., Kim, K.I., Kim, J.-H., 2019. Toxic effects of lead exposure on bioaccumulation, oxidative stress, neurotoxicity, and immune responses in fish: A review. Environmental Toxicology and Pharmacology 68, 101-108. DOI: 10.1016/j.etap.2019.03.010 Mansouri, B., Azadi, N., Habibi, Y., 2015. Study the concentration of heavy metals and some chemical parameters in water distribution network of Sanandaj city in 2014. Jorjani Biomedicine Journal 3(2), 80-88. Mattson, M.P., 2008. Hormesis defined. Ageing research reviews 7(1), 1-7. DOI: 10.1016/j.arr.2007.08.007 Moniruzzaman, M., Das, D., Dhara, A., & Chakraborty, S. B., 2020. Enzymatic, non-enzymatatic antioxidant levels and heat shock protein expression as indicators of metal induced toxicity and reproductive modulation in female indian major carp Cirrhinus cirrhosus. Bulletin of Environmental Contamination and Toxicology 104, 235-244. DOI: 10.1007/s00128-019-02766-z Nguyen, T.Q., Sesin, V., Kisiala, A., Emery, R.N., 2021. Phytohormonal roles in plant responses to heavy metal stress: Implications for using macrophytes in phytoremediation of aquatic ecosystems. Environmental Toxicology and Chemistry 40(1), 7-22. DOI: 10.1002/etc.4909 Paul, N., Chakraborty, S., Sengupta, M., 2014. Lead toxicity on non-specific immune mechanisms of freshwater fish Channa punctatus. Aquatic Toxicology 152, 105-112. DOI: 10.1016/j.aquatox.2014.03.017 Qian, B., Xue, L., Qi, X., Bai, Y., Wu, Y., 2020. Gene networks and toxicity/detoxification pathways in juvenile largemouth bass (Micropterus salmoides) liver induced by acute lead stress. Genomics 112(1), 20-31. DOI: 10.1016/j.ygeno.2019.06.02 Rajeshkumar, S., Mini, J., Munuswamy, N., 2013. Effects of heavy metals on antioxidants and expression of HSP70 in different tissues of Milk fish (Chanos chanos) of Kaattuppalli Island, Chennai, India. Ecotoxicology and Environmental Safety 98, 8-18. DOI: 10.1016/j.ecoenv.2013.07.029 Wang, C.R., Tian, Y., Wang, X. R., Yu, H. X., Lu, X. W., Wang, C., Wang, H., 2010. Hormesis effects and implicative application in assessment of lead-contaminated soils in roots of Vicia faba seedlings. Chemosphere 80(9), 965-971. DOI: 10.1016/j.chemosphere.2010.05.049 Wang, C., Tian, Y., Wang, X., Geng, J., Jiang, J., Yu, H., Wang, C., 2010. Lead-contaminated soil induced oxidative stress, defense response and its indicative biomarkers in roots of Vicia faba seedlings. Ecotoxicology 19, 1130-1139. DOI: 10.1007/s10646-010-0496-x Xu, C., Xue, M., Jiang, N., Li, Y., Meng, Y., Liu, W., Zhou, Y., 2023. Characteristics and expression profiles of MHC class Ⅰ molecules in Carassius auratus. Fish & Shellfish Immunology 137, 108794. DOI: 10.1016/j.fsi.2023.108794 Yoshida, R., 2014. MHC class I recognition by monocyte - macrophage-specific receptors. Advances in Immunology 124, 207-247. DOI: https://doi.org/10.1016/B978-0-12-800147-9.00007-8 Zhao, L., Zheng, Y.G., Feng, Y.H., Li, M.Y., Wang, G.Q., Ma, Y.F., 2020. Toxic effects of waterborne lead (Pb) on bioaccumulation, serum biochemistry, oxidative stress and heat shock protein-related genes expression in Channa argus. Chemosphere 261, 127714. DOI: 10.1016/j.chemosphere.2020.127714 | ||
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