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پویش پیوستگی ژنومی جهت شناسایی ژنهای کاندیدا و مسیرهای زیستی مرتبط با مقاومت به بیماری سل گاوی بر پایه آنالیز غنیسازی مجموعههای ژنی | ||
| علوم دامی ایران | ||
| دوره 56، شماره 3، مهر 1404، صفحه 707-722 اصل مقاله (1.51 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijas.2025.386966.654049 | ||
| نویسندگان | ||
| محسن عبداللهی؛ محمدحسین مرادی* ؛ امیرحسین خلت آبادی فراهانی؛ حسین محمدی | ||
| گروه علوم دامی، دانشکده کشاورزی و محیط زیست، دانشگاه اراک، اراک، ایران | ||
| چکیده | ||
| شناسایی مسیرهای زیستی و ژنهای مرتبط با بیماریها یکی از مهمترین اهداف برنامههای اصلاحنژادی در گاو شیری است. پژوهش حاضر به منظور مطالعه پویش پیوستگی ژنومی جهت شناسایی ژنهای کاندیدا و مسیرهای زیستی مرتبط با مقاومت به بیماری سل گاوی بر پایه آنالیز غنیسازی مجموعههای ژنی انجام شد. به این منظور از اطلاعات مجموع، 1355 رأس گاو هلشتاین (685 حیوان بیمار و 670 حیوان شاهد) از 178 گله استفاده شد که پس از انجام مراحل مختلف کنترل کیفیت، اطلاعات ژنوتیپی 592 نمونه گاو بیمار و 559 نمونه گاو سالم در آنالیزهای نهایی استفاده شدند. تعیین ژنوتیپ این حیوانات با استفاده از تراشههای ژنومیIllumina BovineHD 700k BeadChip برای 727252 جایگاه نشانگری SNP انجام شد. پویش پیوستگی ژنومی (GWAS) با استفاده از مدلهای خطی مختلط لجستیک مورد بررسی قرار گرفت و مناطقی از ژنوم که در سطح 05/0 با بیماری سل گاوی در ارتباط بودند به منظور شناسایی ژنهای کاندیدا و مسیرهای زیستی مرتبط با مقاومت به بیماری سل گاوی بر پایه آنالیز غنیسازی مجموعههای ژنی استفاده شد. نتایج این تحقیق در نهایت منجر به شناسایی مسیرهایی، از قبیل مسیرهای تنظیم مثبت فرآیند آپوپتوز، سازماندهی اتصالات سلولی، تنظیم منفی تولید سیتوکین و تنظیم پاسخ دفاعی شد که به طور معنیداری با بیماری سل گاوی در ارتباط بودند. بررسی این مسیرها نشان داد که اغلب آنها با سیستم ایمنی، کنترل استرس و مقاومت در برابر بیماریها در ارتباط هستند. همچنین ارزیابی عملکرد ژنهای موجود در این مسیرها نشان داد که ارتباط مستقیم برخی از این ژنها مانند SLC11A1، SLC2A2 و CD80 و غیر مستقیم ژنهایی مانند BCL6، HSPD1، KCNMA1، SPP1، CD24، MYO1B، CCL20، و LCP2 با مقاومت به بیماریها و سل گاوی در تحقیقات گذشته نیز تأیید شده است. در مجموع، با توجه به اهمیت شناسایی جایگاههای ژنی تأثیرگذار بر سل گاوی از دیدگاه علمی و اقتصادی، نتایج این تحقیق نشان میدهد مقاومت به بیماری سل گاوی با مسیرهای زیستی و ژنهای کاندیدای مرتبط با سیستم عصبی، ایمنی ذاتی، پاسخ به التهابات، تنظیم پاسخ ایمنی، اتصالات سلولی و هموستازی گلوکز ارتباط نزدیکی دارد. | ||
| کلیدواژهها | ||
| پویش کل ژنومی؛ غنیسازی مجموعه ژنی؛ سلگاوی؛ گاو شیری هلشتاین | ||
| عنوان مقاله [English] | ||
| Genome-wide association study to identify genes and biological pathways related to resistance to bovine tuberculosis based on gene set enrichment analysis | ||
| نویسندگان [English] | ||
| Mohsen Abdollahi؛ Mohammad Hossein Moradi؛ Amir Hossein Khaltabadi Farahani؛ Hossein Mohammadi | ||
| Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran. | ||
| چکیده [English] | ||
| Identifying biological pathways and genes associated with diseases is one of the most important goals of breeding programs in dairy cattle. The aim of the present study was to conduct a genome-wide association study (GWAS) to identify the genes and biological pathways related to resistance to bovine tuberculosis based on gene set enrichment analysis. In total, the information of 1355 cows (consisting 685 cases and 670 controls) from 178 herds were used, of which 592 cases and 559 controls (healthy cows) were passed different quality control steps. These animals were genotyped by using Illumina BovineHD 700k BeadChip for 727252 SNP markers. Genome-wide association study (GWAS) was investigated using mixed linear logistic models, and regions of the genome that were associated with bovine tuberculosis at the 0.05 level were used for identifying the candida genes and biological pathways associated with bovine tuberculosis based on gene set enrichment analysis. The results of this study finally led to the identification of the pathways such as positive regulation of apoptotic process, cell junction organization, negative regulation of cytokine production and regulation of defense response that were significantly associated with bovine tuberculosis. Further investigation of these pathways showed that most of them are related to the immune system, stress and disease resistance. Also, study of function of the genes that were located in these pathways revealed that the direct association of some of these genes, such as SLC11A1, SLC2A2, and CD80, and the indirect association of genes such as BCL6, HSPD1, KCNMA1, SPP1, CD24, MYO1B, CCL20, and LCP2 with disease resistance and bovine tuberculosis has also been confirmed in previous studies. In general, due to the importance of identifying the genes affecting bovine tuberculosis from a scientific and economic point of view, the results of this study show that resistance to bovine tuberculosis is closely related to biological pathways and candidate genes associated with nervous system, innate immunity, inflammatory response, immune response regulation, cell junctions, and glucose homeostasis. | ||
| کلیدواژهها [English] | ||
| Genome-wide association study (GWAS), Gene set enrichment, Bovine Tuberculosis, Holstein dairy cattle | ||
| مراجع | ||
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