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ارزیابی نقش باکتریهای محرک رشد گیاه در کنترل زیستی پاتوژن Salmonella typhimurium | ||
| تحقیقات آب و خاک ایران | ||
| دوره 56، شماره 1، فروردین 1404، صفحه 265-280 اصل مقاله (1.6 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2024.379505.669759 | ||
| نویسندگان | ||
| مطهره عابدین زاده1؛ نعیمه عنایتی ضمیر* 1؛ احسان شکری2؛ شهلا کیان امیری2 | ||
| 1گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 2بخش فناوری نانو، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| پاتوژن‌های انسانی هم‌چون سالمونلا از منابع متعددی وارد خاک‌های کشاورزی می‌شوند. سالمونلا از رایج‌ترین میکروارگانیسم‌های منتقله از غذا می-باشد که عامل ایجاد عفونت‌های مشترک بین انسان و حیوان است. در مطالعه حاضر از برخی باکتریهای محرک رشد گیاه به منظور کنترل پاتوژن Salmonella typhimurium استفاده شده است. نتایج نشان داد که به ترتیب 1) Bacillus rugosus CS5 ، 2)Bacillus vallismortis AS4 ، 3) Priestia aryabhattai CL1 و 4) Bacillus sp. SS4 بیشترین اثر بازدارندگی رشد سالمونلا را در محیط مایع داشتند؛ اما در محیط جامد باکتری-های 1) B. vallismortis AS4 ، 2) B. rugosus CS5 ، 3) P. aryabhattai CL1 و 4) Bacillus sp. SS4 بیشترین بازدارندگی رشد این پاتوژن در محیط جامد را نشان دادند. توانایی تولید سیدروفور، پروتئاز، لیپاز و سیانید هیدروژن توسط این 4 باکتری بررسی شد. هر چهار باکتری دارای توانایی تولید سیدروفور بودند. بیشترین شاخص تولید پروتئاز با مقادیر 9/2 و 5/2 متعلق به B. rugosus CS5 و P. aryabhattai CL1 بود. B. vallismortis AS4 دارای توانایی تولید لیپاز بود. P. aryabhattai strain CL1 و B. rugosus strain CS5 دارای توانایی تولید HCN با مقادیر 48/0 و 2/0 میلی‌گرم در لیتر بودند. تاثیر چهار باکتری به صورت جداگانه و کنسرسیوم میکروبی بر جمعیت سالمونلا در خاک بررسی شد. نتایج نشان داد جمعیت سالمونلا 15 روز پس از تلقیح به خاک تحت تاثیر تیمارها کاهش یافت. بیشترین کاهش جمعیت این پاتوژن مربوط به کنسرسیوم میکروبی و تیمار Bacillus sp. strain SS4 به ترتیب با 92 و 98 درصد کاهش جمعیت نسبت به شاهد بود. نتایج حاکی از تأثیر مثبت باکتریهای محرک رشدی در خاک به منظور کنترل پاتوژن S. typhimurium بود که می‌تواند علاوه بر کاهش اثرات منفی حضور پاتوژن در خاک، سلامت جامعه بشری و بهبود امنیت غذایی را در پی داشته باشد. | ||
| کلیدواژه‌ها | ||
| پروتئاز؛ خاک؛ سالمونلا؛ سیانید هیدروژن؛ سیدروفور | ||
| عنوان مقاله [English] | ||
| Evaluating the role of plant growth-promoting bacteria in biological control of Salmonella typhimurium pathogen | ||
| نویسندگان [English] | ||
| Motahare Abedinzadeh1؛ naeimeh enayatizamir1؛ Ehsan Shokri2؛ shahla kianamiri2 | ||
| 1Department of Soil science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran. | ||
| 2Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| Human pathogens such as Salmonella enter agricultural soils from various sources. Salmonella is one of the most common food-borne microorganisms and is a causative agent of infections shared between humans and animals. In the current study, some plant growth-promoting bacteria were used to control the pathogen Salmonella typhimurium. The results showed that, in order, 1) Bacillus rugosus CS5, 2) Bacillus vallismortis AS4, 3) Priestia aryabhattai CL1 and 4) Bacillus sp. SS4 had the highest inhibitory effect on the grrowth of Salmonella in liquid medium. However, in solid medium, the bacteria 1) B. vallismortis AS4, 2) B. rugosus CS5, 3) P. aryabhattai CL1 and 4) Bacillus sp. SS4 exhibited the greatest inhibition of this pathogen's growth. The ability to produce siderophores, proteases, lipases, and hydrogen cyanide was investigated in these four bacteria. All four bacteria were capable of producing siderophore. The highest protease production index, with values of 2.5 and 2.9, belonged to P. aryabhattai CL1 and B. rugosus CS5, respectively. B. vallismortis AS4 was capable of producing lipase. Both P. aryabhattai strain CL1 and B. rugosus strain CS5 had the ability to produce HCN, with values of 0.48 mg/mL and 0.2 mg/mL, respectively. The simple impact of four separate bacteria and a microbial consortium on the Salmonella population in soil was investigated. The results showed that the Salmonella population decreased 15 days after inoculation due to the treatments. The greatest reduction in the the population of this pathogen was related to the microbial consortium and the Bacillus sp. strain SS4 treatment, with a 92 % and 98 % reduction in population compared to the control, respectively. The findings indicate a positive effect of plant growth-promoting bacteria in soil for controlling S. typhimurium, which could reduce the negative impacts of the pathogen's presence in soil, thereby enhancing human health and food security. | ||
| کلیدواژه‌ها [English] | ||
| hydrogen cyanide, protease, Salmonella, Siderophore, soil | ||
| مراجع | ||
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