Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing | ||
| Journal of Ultrafine Grained and Nanostructured Materials | ||
| دوره 58، شماره 1، شهریور 2025، صفحه 1-14 اصل مقاله (1.15 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22059/jufgnsm.2025.01.01 | ||
| نویسندگان | ||
| parisa akhlaghi1؛ Rouhollah Mehdinavaz Aghdam* 1؛ Sina Ashouri Sharafshadeh1؛ Yalda Pirhadi Tavandashti2 | ||
| 1Biomaterials Lab, School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran. | ||
| 2Biomaterials Lab, School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran | ||
| چکیده | ||
| Bacterial wound infection delays wound healing and may lead to life-threatening complications. Hence wound dressing materials are not restricted to stop blood loss, but also to protect the wound from bacterial infection and to accelerate the wound healing process. In this study, biopolymeric nanofibers of Silk Fibroin (SF) and decellularized human Amniotic membrane (dehAM) were fabricated by electrospinning technique to be used in wound healing. Thymol, a natural antibacterial substance, solutions containing 5% wt/wt, 10%wt/wt and 15% wt/wt thymol was added to the SF nanofibers to increase the sustained release. Scanning Electron Microscopy and Attenuated Total Reflectance Fourier-Transform Infrared (FTIR-ATR) were used to examine the nanofibers' morphology and chemical characteristics. Water contact angle and water vapor transmission rate of dressings were measured. When the thymol concentration of the dressing was increased, the wettability of the dressing decreased by an average of 54%. The dressings' antimicrobial activity was determined using the colony count method, which dressings effectively inhibited the development of E. coli and S. aureus. Furthermore, cell attachment, cell viability, and in vitro cytotoxicity experiments have demonstrated the biocompatibility and cytocompatibility of the dressings, as well as their suitability for MSCs attachment. Altogether, the produced dressing was shown to have immense potential as a wound dressing. | ||
| کلیدواژهها | ||
| Wound dressing؛ Electrospinning؛ Thymol؛ Human Amniotic Membrane, Antibacterial agent | ||
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