Canine distemper virus genotypes among vaccinated and unvaccinated dogs in Iran: a case-control study | ||
| Iranian Journal of Veterinary Medicine | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از 25 مرداد 1405 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2026.403394.1005922 | ||
| نویسندگان | ||
| Abdolraouf Saberi1؛ Shahram Jamshidi1؛ GOLSHID Javdani Shahedin* 2؛ Seyed Nezam Hoseini3؛ Davood Mosavinasab4؛ Iraj Ashrafi5 | ||
| 1Department of Internal medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran | ||
| 3Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran | ||
| 4WHO Collaborating Center for Reference and Research On Rabies, Department of Rabies, Pasteur Institute of Iran, Tehran, Iran | ||
| 5Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| background: Canine distemper virus (CDV) causes a highly infectious multisystem disease known as canine distemper (CD), which influence diverse species within the order of Carnivora. The virus shows genetic diversity, particularly within the hemagglutinin (H) gene region. The H protein is an important target in molecular studies due to its high divergence. Since vaccination program is conducted in Iran, CD still remains as one of the major causes of death in dogs in this country. Objective: To expand our understanding about the differences among commonly found strains and those ones included in vaccines to reconsider the current vaccination program and predict probable vaccination outcomes. methods: Ocular and Nasal swabs were collected from 16 dogs suspected of CDV infection. PCR was conducted to confirm the detection and sequencing of samples and vaccines using forward and reverse primers. Nucleotide sequences were aligned using Bioedit and CLC Genomics Workbench. Phylogenetic analysis was carried out using MEGA 11 software programme Results: Based on the phylogenetic analysis all the isolated strains were included in the Arctic lineages. Evaluation of the deduced amino acid sequences indicated substitutions in three amino acid residues compared to the Onderstepoort vaccine strain and Biocan one. Alignment of these wild 16 H gene nucleotide sequences revealed identity level ranges from 99.8% to 99.9% compared to Onderstepoort vaccine strain (Table2). Similarity (99.84%-99.85%) was detected between Iranian H sequences and Biocan vaccine strain Conclusion: The strains were isolated in the Arctic lineages. Due to the variation in the H gene sequences between the vaccine strains and the wild strains, the CDV lineages currently circulating in Iran differ from the identified lineages; America-1. We conducted this study to deepen our understanding in regard to differences among circulating and vaccine strains and predicting whether the reconsideration of vaccine program is warranted. | ||
| کلیدواژهها | ||
| Canine distemper virus؛ Iran؛ Lineages؛ Phylogenetic؛ Vaccine | ||
|
آمار تعداد مشاهده مقاله: 84 |
||