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مطالعه روند ایجاد تغییرات سلولی در کشتهای سلولی آلوده به پستی ویروس ها به روش ایمونوفلورسانس مستقیم | ||
| مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
| مقاله 14، دوره 59، شماره 2 - شماره پیاپی 618، مرداد 1383 اصل مقاله (284.41 K) | ||
| نویسندگان | ||
| دکتر فرهید همت زاده؛ دکتر هادی کیوانفر؛ دکتر مریم بادوام؛ محمد طاهری* | ||
| کلیدواژه‌ها | ||
| ایمونوفلورسانس؛ پستی ویروس؛ سویه سیتوپاتوژن؛ سویه غیر سیتوپاتوژن؛ کشت سلول | ||
| عنوان مقاله [English] | ||
| - | ||
| چکیده [English] | ||
| Objective: This study was carried out to find the progressive cellular changes developed in cell culture with cytopathic (cp) and noncytopathic (ncp) pestiviruses isolated from cow and sheep. Design: Observational study. Procedure: Following inoculating the R-BK cell cultures in Lighton tube with NADL strain of BVD, a cp strain of Border disease virus and a nocytopathic BVD, the cells were stained with FA and observed with IF microscope. In inoculated cultures with cp and ncp pestivirus obvious changes were present at the first day and multiple focal expression of viral antigens were seen. Results: In the second day in cp inoculated cultures, the morphological changes began and antigenic spread were more intensive. In the 3rd day the CPE foci were seen in different parts of the cultured cells with high concentration of antigen around the CPE sites. The cells started to detach and became rounds and finally in the 4th and 5th day, almost all cells were antigen positive and developed CPE. However, those cultures inoculated with noncytopathic strain of BVD, in daily observation the antigenic spread was too slow and up to 6 days post inoculation no CPE were observed. | ||
| کلیدواژه‌ها [English] | ||
| cell culture, Cytopathic strain, Immunoflurescence, Noncytopathic strain, Pestivirus | ||
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