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ایجاد ماده زایی در ماهی قزل آلای رنگین کمان توسط پرتو فرابنفش | ||
| مجله منابع طبیعی ایران (منتشر نمی شود) | ||
| مقاله 5، دوره 54، شماره 4 - شماره پیاپی 940، تیر 1380 اصل مقاله (747.96 K) | ||
| نویسندگان | ||
| قباد آذری تاکامی؛ حمید فرحمند؛ برزان بهرامی کمانگر* | ||
| کلیدواژهها | ||
| اسپرم؛ پرتو فرابنفش و ژنوم؛ قزل آلای رنگین کمان؛ ماده زایی | ||
| عنوان مقاله [English] | ||
| - | ||
| چکیده [English] | ||
| In order to induce meiosis gynogenesis in rainbow trout (Oncorhynchus mykiss), spermatozoa genome was destroyed by Uv irradiation (2887 J.J w/cm2) and after fertilization with intact ovum, diploidy was returned by applying a thermal shock (27 + O.5°c) for 10 minutes. Thermal shock was applied at 30 and 40 minutes after fertilization. Uv-irradiation was supplied from a germicidal lamp (30w) with maximum intensity at 245nm. Before irradiation, sperm was diluted with two kinds of solutions at a ratio of 1:4 and then subjected to 1,3,5,8 and 15 minutes of irradiation. Statistical design for comprising effects on 3 different treatment groups were observed as split split plot on gynogenesis and split plot on haploid and randomized complete block on control. Golden color in rainbow trout was used as a marker for distinguishing gynogenetic offspring. Furthemore, for confirmed diploidy chromosome slides were prepared. The best performance was achieved in treatment of 8 minute irradiation and applied thermal shock of 30 minutes after fertilization, but there was no significant difference between the two solutions (P>0.O5). The mean percentage gynogenesis was 60.61 % in the treatments of three replications. Neverthless, 100% gynogenesis was achieved from 8 minute irradiation in the second replication. | ||
| کلیدواژهها [English] | ||
| Genome, gynogenesis, Rainbow trout, Spermatozoa, Uv-irradiation | ||
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