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A Thermodynamic Study of the Interaction between Urease and Copper Ions | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 2، دوره 21، شماره 1، خرداد 2010 اصل مقاله (253.5 K) | ||
| نویسندگان | ||
| A.A. Saboury؛ Elaheh Poorakbar-Esfahani؛ G. Rezaei-Behbahani | ||
| چکیده | ||
| A thermodynamic study of copper ions by jack bean urease (JBU) was carried out at two temperatures of 27 and 37?C in Tris buffer (30 mM; pH=7.0) using an isothermal titration calorimetry. There is a set of twelve identical and non-interacting binding sites for copper ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 285 µM and ?15.2 kJ/mol at 27?C and 346 µM and ?14.6 kJ/mol at 37?C, respectively. The molar entropy of binding is ?17.2 J K-1 mol-1at 27?C and +19.1 J K-1 mol-1 at 37?C. Hence, the binding process of copper ion to JBU is not only enthalpy driven but also it is entropy driven, which the role of entropy driven should be more effective by increasing the temperature. | ||
| کلیدواژه‌ها | ||
| Copper ion؛ Enthalpy of binding؛ Entropy of Binding؛ Isothermal titration calorimetry؛ Urease | ||
| عنوان مقاله [English] | ||
| A Thermodynamic Study of the Interaction between Urease and Copper Ions | ||
| نویسندگان [English] | ||
| A.A. Saboury؛ Elaheh Poorakbar-Esfahani؛ G. Rezaei-Behbahani | ||
| چکیده [English] | ||
| A thermodynamic study of copper ions by jack bean urease (JBU) was carried out at two temperatures of 27 and 37?C in Tris buffer (30 mM; pH=7.0) using an isothermal titration calorimetry. There is a set of twelve identical and non-interacting binding sites for copper ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 285 µM and ?15.2 kJ/mol at 27?C and 346 µM and ?14.6 kJ/mol at 37?C, respectively. The molar entropy of binding is ?17.2 J K-1 mol-1at 27?C and +19.1 J K-1 mol-1 at 37?C. Hence, the binding process of copper ion to JBU is not only enthalpy driven but also it is entropy driven, which the role of entropy driven should be more effective by increasing the temperature. | ||
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