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Masoudipour, Elham, Mousavi, Seyed Latif, Basiri, Mohsen. (1390). Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay. , 1(1), 11-25. doi: 10.22059/pbs.2011.22454
Elham Masoudipour; Seyed Latif Mousavi; Mohsen Basiri. "Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay". , 1, 1, 1390, 11-25. doi: 10.22059/pbs.2011.22454
Masoudipour, Elham, Mousavi, Seyed Latif, Basiri, Mohsen. (1390). 'Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay', , 1(1), pp. 11-25. doi: 10.22059/pbs.2011.22454
Masoudipour, Elham, Mousavi, Seyed Latif, Basiri, Mohsen. Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay. , 1390; 1(1): 11-25. doi: 10.22059/pbs.2011.22454

Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay

Progress in Biological Sciences
مقاله 2، دوره 1، شماره 1، شهریور 2011، صفحه 11-25    اصل مقاله (66.8 K)
شناسه دیجیتال (DOI): 10.22059/pbs.2011.22454
نویسندگان
Elham Masoudipour1؛ Seyed Latif Mousavi2؛ Mohsen Basiri3
1Khatam University-P.H.D
2Shahed University-P.H.D
3Tarbiat Modares University
چکیده
Development of potent new anti-Shigella agents for rapid and specific detection and treatment is
of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range
of non-nucleic acid targets, may be of value for this purpose. In the present study, we used a Systematic
Evolution of Ligands by Exponential enrichment (SELEX) process to select DNA aptamers
that bind to whole S. sonnei cells. The resulting aptamers exhibited specificity in binding only
to S. sonnei cells. Five unique DNA sequences were isolated from the aptamer cocktail by cloning,
among which ASA4 showed the highest affinity. © 2011 Progress in Biological Sciences, Vol. 1,
No.1, 11-15.
کلیدواژه‌ها
DNA aptamer؛ ELASA؛ Exponential؛ SELEX؛ Shigella sonnei
عنوان مقاله [English]
Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay
نویسندگان [English]
Elham Masoudipour1؛ Seyed Latif Mousavi2؛ Mohsen Basiri3
چکیده [English]
Development of potent new anti-Shigella agents for rapid and specific detection and treatment is
of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range
of non-nucleic acid targets, may be of value for this purpose. In the present study, we used a Systematic
Evolution of Ligands by Exponential enrichment (SELEX) process to select DNA aptamers
that bind to whole S. sonnei cells. The resulting aptamers exhibited specificity in binding only
to S. sonnei cells. Five unique DNA sequences were isolated from the aptamer cocktail by cloning,
among which ASA4 showed the highest affinity. © 2011 Progress in Biological Sciences, Vol. 1,
No.1, 11-15.
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