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تشخیص آنتی ژن های توموری در عقده لمفاوی گاو مبتلا به لنفوسارکوم ویروسی با روش رسوب ایمنی | ||
| مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
| مقاله 8، دوره 63، شماره 2 - شماره پیاپی 725593، تیر 1387، صفحه 141-146 اصل مقاله (261.16 K) | ||
| نویسندگان | ||
| غلامرضا نیکبخت بروجنی؛ سید مهدی امام؛ ندا برجسته | ||
| گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران | ||
| چکیده | ||
| In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and 26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis. | ||
| کلیدواژه‌ها | ||
| آنتی ژن های توموری؛ رسوب ایمنی؛ لکوزگاوی؛ لمفوسارکوم | ||
| عنوان مقاله [English] | ||
| DETECTION OF TUMOR ANTIGENS IN BOVINE VIRAL LYMPHOSARCOMA BY IMMUNOPRECIPITATION METHOD | ||
| نویسندگان [English] | ||
| gholamraza nikbakhat؛ Seyyed Mahdi Emam؛ Neda Barjesteh | ||
| چکیده [English] | ||
| In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and 26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis. | ||
| کلیدواژه‌ها [English] | ||
| bovine leucosis, immunoprecipitation, Lymphosarcoma, tumor antigens | ||
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