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Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36 | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 1، دوره 23، شماره 1، خرداد 2012، صفحه 7-13 اصل مقاله (627.95 K) | ||
| نویسنده | ||
| E. Omidinia | ||
| Pasteur Institute of Iran | ||
| چکیده | ||
| The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) protocol involving 22% (w/w) polyethylene glycol (PEG)-10,000, and 18% (w/w) citrate with a yield of 39.7%, specific activity of 2600 U/mg and purification factor of 4.8. It was shown to have a molecular weight of 40 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purified thermophile enzyme was stable in alkaline pH range (9.0-11.0) with the optimum pH of 9.0. It was highly stable at 60 °C and retained 100% activity even after 90 minutes, suggesting that it belong to the family of thermophilous. Collectively, our obtained data revealed that the thermophilic protease produced by B. subtilis BP-36 has the potential application in industrial processes under high temperature. | ||
| کلیدواژهها | ||
| Alkaline protease؛ Thermophilic؛ Bacillus subtilis؛ isolation؛ purification | ||
| عنوان مقاله [English] | ||
| Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36 | ||
| نویسندگان [English] | ||
| E. Omidinia | ||
| چکیده [English] | ||
| The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) protocol involving 22% (w/w) polyethylene glycol (PEG)-10,000, and 18% (w/w) citrate with a yield of 39.7%, specific activity of 2600 U/mg and purification factor of 4.8. It was shown to have a molecular weight of 40 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purified thermophile enzyme was stable in alkaline pH range (9.0-11.0) with the optimum pH of 9.0. It was highly stable at 60 °C and retained 100% activity even after 90 minutes, suggesting that it belong to the family of thermophilous. Collectively, our obtained data revealed that the thermophilic protease produced by B. subtilis BP-36 has the potential application in industrial processes under high temperature. | ||
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آمار تعداد مشاهده مقاله: 7,623 تعداد دریافت فایل اصل مقاله: 4,599 |
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