A survey of embryonic development of swissmice knee joint in vitro | ||
| مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
| Article 11, Volume 53, 1,2 - Serial Number 1000149, August 1998 PDF (1.88 M) | ||
| Abstract | ||
| In order to study the development of knee joint in absence of movement; mouse limb buds were separated from 15 days old embryos and cultured in Ham's F-IO medium conditioned with 37°C and 5% CO2 for 1 to 6 days. The medium was renewed every 24h during incubation. After 6 days, specimens were fixed by Buin's fluid, embedded in paraffin wax, serially sectioned and stained with H&E. The results indicated that, the mesenchymal cellsof joint interzone were differentiated to fibroblast like cells and chondrocytes rather than lossing to form joint space. The intra-articular structures and joint capsules were also failed to develop in culture condition. In conclusion it can be postulated that, the absence of movement and in vitro conditions could affect the joint development and its intra-articular structures. | ||
|
Statistics Article View: 2,048 PDF Download: 894 |
||