AMPEROMETRIC MICROBIOSENSOR FOR THE DETERMINATION OF CHOLINE | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 3، دوره 9، شماره 1، 1998 اصل مقاله (385.71 K) | ||
| چکیده | ||
| The construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. The procedure of choline determination involved the electrochemical detection of enzymatically generated hydrogen peroxide. This microsensor has a linear response between 0.038-0.1 mM choline chloride. The response time is 1-2 minutes. The optimal working conditions for the microsensor were 0.1 M TRIS-HCl, pH = 8 at 30°C | ||
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