پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 127 |
| تعداد شمارهها | 7,174 |
| تعداد مقالات | 77,100 |
| تعداد مشاهده مقاله | 156,321,185 |
| تعداد دریافت فایل اصل مقاله | 117,889,476 |
Evidence for the Essential Arginine and Histidine Residues in Catalytic Activity of Glucose 6-Phosphate Dehydrogenase from Streptomyces aureofaciens | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 3، دوره 16، شماره 1، خرداد 2005 اصل مقاله (124.4 K) | ||
| چکیده | ||
| Glucose 6-phosphate dehydrogenase (G6PD) was purified from Streptomyces aureofaciens and inactivated with butanedione and diethylpyrocarbonate. Incubation of the enzyme with butanedione resulted in a rapid activity loss (80%) within 5 min, followed by a slow phase using a molar ratio to enzyme concentration of 100. Fluorescence studies showed a conformational change in the butanedione-modified enzyme. NAD+, NADP+ and glucose 6-phosphate protected the enzyme against inactivation. Diethylpyrocarbonate (2 mM) completely inactivated the enzyme after 2 min. Stoichiometry of the inactivation showed 2 moles of histidine residues per mole of enzyme with complete activity loss. Maximum emission spectrum of the enzyme decreased (23%) upon modification and the presence of NAD+ or NADP+ further decreased the fluorescence by 27% and 10.5%, respectively. The data suggest that essential arginine and histidine residues may be involved in the catalytic activity of Streptomyces aureofaciens G6PD | ||
| عنوان مقاله [English] | ||
| - | ||
| چکیده [English] | ||
| - | ||
|
آمار تعداد مشاهده مقاله: 1,129 تعداد دریافت فایل اصل مقاله: 1,330 |
||
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب