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PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS) | ||
Journal of Sciences, Islamic Republic of Iran | ||
مقاله 1، دوره 12، شماره 2، شهریور 2001 اصل مقاله (208.91 K) | ||
چکیده | ||
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolation. STS also decreased the internode length of potato shoots but increased dry weight and chlorophyll. Regenerated plants were obtained from cultured protoplasts in a series of media based on the MS medium. Protoplasts of Delaware showed a better response in cell division and colony formation in agarose-solidified culture medium. Regenerated plants showed some degrees of aneuploid but basically were similar to the original potato plants. | ||
عنوان مقاله [English] | ||
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چکیده [English] | ||
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