CHANGES IN GLUTATHIONE S-TRANSFERASE ACTIVITY AND ZEARALENONE CONTENT IN SUSCEPTIBLE AND TOLERANT WHEAT HEADS INOCULATED WITH FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF FUSARIUM HEAD SCAB | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 2، دوره 11، شماره 3، 2000 اصل مقاله (198.05 K) | ||
| چکیده | ||
| Glutathione S-transferase (GST) activity pattern was determined in tolerant (cv. Sumai#3) and susceptible (cv. Falat) wheat heads inoculated with Fusarium graminearum, the causal agent of head scab disease (FHB), during various developmental stages. GST specific activity exhibited a transient pattern in Sumai#3 reaching a maximum level at the milk stage and declining thereafter. GST level in Sumai#3 was three times greater than that in the susceptible cultivar. Thin layer chromatography and densitometry were used to measure zearalenone (ZEN) content in FHB-inoculated wheat heads. Zearalenone concentration reached a maximum level in Sumai#3 at the milk stage and thereafter it declined. ZEN content in the susceptible wheat heads continued to increase and reached a maximum level at the ripening stage. There seems to be a correlation between the increased GST activity and significantly lower ZEN production in the tolerant wheat heads. | ||
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